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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaaatcgtctctgtgatgttggcacgcccagcaattttccttgcccacatacatcatcataacgcatgaaccagtgcatttaatagtggcataagactcaagaatatgtcatgtcttttcttttcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA

Basic information

species Zea mays
transcript GRMZM2G358830_T02
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G358830_T02 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g54700.1 (Oryza sativa), 3'ss of exon 5
gtaaat-cgtctctgtgatgttggcacgcccagcaattttccttgcccacatacatcatcataacgcatgaaccagtgcatttaatagtggcataagactcaagaatatgtcatgtcttttcttttcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA
|||||  | || |  | |      |||  | | |||||   ||||| | | ||| |||  | |   | |    |||||||   |  |   | |  | |||        |||  ||||      ||   ||| |||||| || || |||||||| |||||||| |||||||| |||||||| |||||||||||||| |||||||||||||| ||||| |||||||| || | ||| 
gtaaacacatcaccatcacaac--cacaacaatcaattacacttgcactcgtacgtca--aaagttcct----cagtgcacgcaccacgcggactaaactt-------tgtgctgtcaacaacttgtatctccagATATTTAGCTCCAGAGTATGCTGCCACGGGCAAAGTGAACGACCGTTCCGACGTCTTCTCATTCGGCGTGATGCTCCTGGAGCTGATCACCGGGAAGAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149104119|gb|EE289551.2|EE289551
EST:     CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
EST: gi|71769046|gb|DR966983.1|DR966983
EST:     CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
EST: gi|149084848|gb|EE174353.2|EE174353
EST:     CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
EST: gi|76926939|gb|DV171065.1|DV171065
EST:     CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
EST: gi|148952361|gb|EC898175.2|EC898175
EST:     CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
EST: gi|148929996|gb|EE285849.2|EE285849
EST:     CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA
EST: gi|76017809|gb|DT944979.1|DT944979
EST:     CAAGCAGCTGAAGTAACCTCTGTTTATACGCGTGTAATGGGGACTTTCGG                         ATACTTGGCGCCAGAGTACGCTTCCACAGGCAAAGTTAACGACCGGTCCGA
genomic: CAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 29 (upstream exon), 38 (downstream exon)
Score: 9

GTTGCAGATTTTGGGTTGGCCAAATACCAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA


Block sizes: 49 (upstream exon), 43 (downstream exon)
Score: 78

GTTGCAGATTTTGGGTTGGCCAAATACCAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 198

GTTGCAGATTTTGGGTTGGCCAAATACCAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA


Block sizes: 49 (upstream exon), 38 (downstream exon)
Score: 41

GTTGCAGATTTTGGGTTGGCCAAATACCAAGCAGCTGAAGTAACCTCTGTTTCTACGCGTGTAATGGGGACTTTCGGgtaaatcgtc ... tcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttaatagtggcataagactcaagaatatgtcatgtcttttcttttcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA
 atttaat  putative branch site (score: 41)
                              tcatgtcttttctttt  putative PPT
                                     ttttctttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaaatcgtctctgtgatgttggcacgcccagcaattttccttgcccacatacatcatcataacgcatgaaccagtgcatttaatagtggcataagactcaagaatatgtcatgtcttttcttttcgtcttcagATACTTGGCGCCAGAGTACGCTGCCACAGGCAAAGTTAACGACCGGTCCGACGTCTTCTCCTTCGGCGTGATGCTTCTGGAACTGATCACTGGAAGGAAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA