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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...aaatcgactaagtttatttagtcatttgtatgattgtttgctcgcattgacctttcttgttttccgtattcattcactagcaacaatattaattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG

Basic information

species Zea mays
transcript GRMZM2G180335_T03
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G180335_T03 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g69130.1 (Oryza sativa), 3'ss of exon 12
aaatcgactaagtttatttagtcatttgtatgattgtttgctc-gcattgac---ctttcttgttttccgtattcattcactagcaacaatattaattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG
  || |   | |||| |||  ||   ||  | | | ||  ||  |||| ||    || |  | |||| | |  ||||  ||  |||||  ||||  | || ||||  ||||||||||||||||| || |||| ||| || || |||||||||||||||||| ||||||||||||||||||||||| | | |  | | || ||||
tgatagctgatgttttttt--tcccctgatttactcttgactttgcatagatatgctataatctttttcattgtcata-acctgcaactctattt-tggttcagGCGGATTACATAAACACATCTCATCCAAATTTCATCGGCGGCAGCAAGGCTGTTGAGCAAGCACAGCAGCAAGTTAGGTCTTCTAGACTGGCAGCTGTGG

upper sequence: GRMZM2G180335_T03 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA15G06380.1 (Glycine max), 3'ss of exon 12
aaatcgactaagtttattta-gtcatttgt-atgattgtttgctcgcattgacctttcttgttttccgtattcat--tcactagcaacaatatt-aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG
      | | || ||||| | |||| |||  ||| |   |  ||  ||  |    |  |   ||||| | | |||  |  ||      | || | |||| |||||   || ||||||||||| || ||||||| |||||||||||| ||||||||  | ||   |||    |||||    | |||  | |      ||  |   |
-----cagtcagcttattcatgtcaattgcgatggtcagtctctatcagagtgaatcatcaatttccatgtccatgatttctttttgaactactcaattctgcagATGGACTACATAAACACTTCCCACCCAAATTTTATTGGTGGAAGCAAGGCATTAGAAATAGCTGCACAGCAGACCAAGTCGTCTAGGGTTGCTATACCAG

upper sequence: GRMZM2G180335_T03 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA13G32940.1 (Glycine max), 3'ss of exon 12
aaatcgactaagtttattta-gtcatttgt-atgatt-gtttgctcgcat---tgacctttcttgttttccgtattcattcactagcaacaatattaattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG
      | | || ||||| |  ||| |||| ||| |  || |  | |  |   | | |  | |   | ||| |  |   | | | | ||| |  | |||| |||||   || ||||||||||| || ||||||| |||||||||||| ||||||||  | ||   |||   ||| ||    | |||  | |      ||  |   |
----tcagtcagcttattcatatcaattgtgatggtcagtctattagagtgaataatcaatttcaatgtccatgatttcttatttg-aactac-tcaattatgcagATGGACTACATAAACACTTCCCACCCAAATTTTATTGGTGGAAGCAAGGCATTAGAAATAGCTGCGCAACAGACCAAGTCCTCTAGGGTTGCTATACCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|74238214|gb|DT646128.1|DT646128
EST:     ATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAG                         GAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT
genomic: ATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT
EST: gi|32845999|gb|CD985680.1|CD985680
EST:     ATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAG                         GAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT
genomic: ATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT
EST: gi|211302452|gb|FL244058.1|FL244058
EST:     TATCACACATTATTGAGATGGAG                         GAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTTGCAGCAAGGCT
genomic: TATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT
EST: gi|78087100|gb|DV515493.1|DV515493
EST:     ATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAG                         GAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT
genomic: ATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 117

TCCTATACTCCGCAGAAGCATGGATGAAGTCATTGGGAAGTTTTTGCGGGATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 121

TCCTATACTCCGCAGAAGCATGGATGAAGTCATTGGGAAGTTTTTGCGGGATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 123

TCCTATACTCCGCAGAAGCATGGATGAAGTCATTGGGAAGTTTTTGCGGGATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 172

TCCTATACTCCGCAGAAGCATGGATGAAGTCATTGGGAAGTTTTTGCGGGATGGACTTAAGCCAGCAGAAAGTATGATATCACACATTATTGAGATGGAGgtgcacaccc ... aattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

attgacctttcttgttttccgtattcattcactagcaacaatattaattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG
                                         tattaat  putative branch site (score: 172)
                                    aacaatattaattgt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
aaatcgactaagtttatttagtcatttgtatgattgtttgctcgcattgacctttcttgttttccgtattcattcactagcaacaatattaattgtgcagGAAGATTACATAAACACATCGCACCCAAGTTTTATTGGTGGCAGCAAGGCTGTTGAGGAAGCACAGCAGCAAGTTAGGTCTGCCAAAACGTCTGCAGTGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGCAG