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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgtttatttcataattgatttttgtaatgatattgcagtctatggttcattctttttaatttttaggttatgtttagcattttgttcaaaatatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG

Basic information

species Zea mays
transcript GRMZM2G177970_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G177970_T02 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g54020.1 (Oryza sativa), 3'ss of exon 4
---tgtttatttcataattgatttttgtaatgatattgcagtc--tatggttcattctttttaatttttaggttatgtttagcattttgttcaaaatatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG
   || ||  ||  || || || || | |||  | ||   |||  || | ||||||  || |  |  || |  || | ||| ||  ||||| ||||| |||| ||||||| ||||||||||| || |||| | | || |||||||||||||||||||||||||||||||| || |||||| | ||||| || |||
gtctgatttgtttgtagttcatatt-gcaattttttttttgtctttacgattcatt--ttgtggtcctttg--taagcttatcacgttgtttaaaatgtctgcagATTACGTTACCATATGAAGCACCACAGTTGTCAAAAGCAACAAGAAGCACTGCTGCAATATATCTGGCTTGTGGCATTGACAGTTCAAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76294199|gb|DV033767.1|DV033767
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
EST: gi|211458161|gb|FL127573.1|FL127573
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
EST: gi|50331394|gb|CO526520.1|CO526520
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
EST: gi|60398533|gb|DN231349.1|DN231349
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCTCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
EST: gi|211458162|gb|FL127574.1|FL127574
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGC
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGC
EST: gi|78120428|gb|DV538812.1|DV538812
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
EST: gi|71449704|gb|DR830754.1|DR830754
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
EST: gi|211458163|gb|FL127575.1|FL127575
EST:     GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCA                         ATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT
genomic: GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 42 (upstream exon), 49 (downstream exon)
Score: 16

GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG


Block sizes: 42 (upstream exon), 48 (downstream exon)
Score: 48

GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG


Block sizes: 28 (upstream exon), 36 (downstream exon)
Score: 7

GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG


Block sizes: 42 (upstream exon), 46 (downstream exon)
Score: 13

GATTCATATGAGACATTCTTTTTCATCGTGGATCTTCATGCAgtaagttctt ... atatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttcattctttttaatttttaggttatgtttagcattttgttcaaaatatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG
        ttttaat  putative branch site (score: 13)
       tttttaatttttaggt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgtttatttcataattgatttttgtaatgatattgcagtctatggttcattctttttaatttttaggttatgtttagcattttgttcaaaatatctgtagATTACTTTACCATATGAGGCGCCACTGCTTTCTAAAGCAACAAGAAGCACTGCTGCAATATATCTTGCATGTGGCGTCGACAGCTCCAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTC
- - - - - - - - - - - - - - - - -tgcagtc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttatgtt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttttgtt