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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttccctggtgtctgatgtttgtctagttagtatgaaatgttgatagaattgtattctattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG

Basic information

species Zea mays
transcript GRMZM2G121200_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G121200_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os09g24320.1 (Oryza sativa), 3'ss of exon 4
gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttccctggtgtctgatgtttgtctagttagtatgaaatgttgatagaattgtattcta-ttttattaaacacaagaa-gattgcatggttat----------atgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG
                                                              | ||||| |||  ||            || | ||| |||||||||| |||| |||||||||||  |    ||| | |   |||||  | ||  ||   ||          |||||||||||||| |||||||||||||  || ||||||||| | |||||  | || |||||||||||||| |||||||  |||||||||
-------------------------------------------------------------gtaggttttttcattgac----------tgcccagtcagtatgaaattttgacagaattgtatttgagcaatatgagaactaagaacaactgtttgtcgatggctcgctcaatgcagGGGGCTATTATGGCTGTTGGAGGATCGAGACCTACTTTGGTGGCCAACAAAGATGGTTTCTTTAGCATCAAGAACGAAATGCTG

upper sequence: GRMZM2G121200_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA01G20720.1 (Glycine max), 3'ss of exon 4
gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttccctggtgtctgatgtttgtctagttagtatgaaatgttgatagaattgtattctattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG
                                         |||  || |||   ||| | |     ||      ||   | |||  |||      ||       | | ||| | | | | |   || |  | |||   |    ||| ||      | || |  |||||||||||||||| |||||||| |||| |||||||||  | || ||||||||||||||||||| | | || ||| ||||||||
----------------------------------------gtgagttggtgaccaaat-gaacaa--ttgaattttgacttttga--tttaatagattgaggctcagttttg-tggcaatttgacctttgattttatttatgttaagcttttccattcatgtttagGGGGCTATCATGGCGGTTGGAGCATCAAAACCTACTGTCCTAGCAGATAAGGATGGTTTCTTTACTGTAAAAAGTAAAATGCTG

upper sequence: GRMZM2G121200_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA20G24830.1 (Glycine max), 3'ss of exon 3
gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttccctggtgtctgatgtttgtctagttagtatgaaatgttg-atagaattgtattctattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG
                                               |||  |     | | ||||  | |     |  ||||  || ||    || |  | ||     |||  | | | | |   |  ||||||          ||  ||||    |||| | ||||| ||||||||||||||||| || ||||  ||||||||  |||||||||| ||||| ||||| ||  | |||| | ||||||||
-----------------------------------------------gttagtgatgatttctgggacttt-----gaagtct--tgatttgagagatgttgtggctcattgtgtggcaatttgaac-gttttatctgggggttcaactttgccct-ttatttccagGGTGCTATCATGGCTGTTGGCGCATCAAAGCCTACTGTTCTGGCTGATAAAGATGGATTCTTCAGCGTGAAGAATAAAATGCTG

upper sequence: GRMZM2G121200_T01 (Zea mays), 3'ss of exon 4
lower sequence: AT3G25860.1 (Arabidopsis thaliana), 3'ss of exon 4
gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttccctggtgtctgatgtttgtctagttagtatgaaatgttgatag-aattgtattctattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG
                                                                             || | |||        |   | |  |    | |  ||||  |||  |    ||||  ||||           ||  |     || |  |||| ||||| |||||||||||||||||||  || |||||||| |||||||||||||| ||||| ||| | || |    |||||||
-----------------------------------------------------------------------------gtatatgaaagaaactttcattgcctcttctatgaatagtgattacaaggaatttacttaattg--------ttatactcaaatctctagGGTGCTATTATGGCTGTTGGAGCGTCAAAGCCAACTGTAGTTGCTGATAAGGATGGATTCTTCAGTGTAAAAAACACAATGCTG

upper sequence: GRMZM2G121200_T01 (Zea mays), 3'ss of exon 4
lower sequence: Vv01s0011g03380.t01 (Vitis vinifera), 3'ss of exon 4
gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttc-cctggtgtctgatgtttgtctagttagtatgaaatgttgatagaattgtattc-tattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG
                                                              || ||| |    || ||  | | |  | | | |||||   || | ||  |  |    || |   | | | | |       |   ||  ||| |  | | |   ||||||||||||||||| |||||||| ||||  |||||||| ||  ||||||| ||||| ||||| ||| | || ||| ||||||||
-------------------------------------------------------------gtgagttgcaaagccaggactttcacctctat-tagtt--cataagattcttctgcccttttgggcatgtgtgagctttttctcaaaatttggaatgatgt---cagGGGGCTATCATGGCCGTTGGAGCTTCAAAGCCTACTGTTGTCACTGATAAAGATGGATTCTTCAGTGTTAAAAGTAAAATGCTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149083381|gb|EE172688.2|EE172688
EST:     TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAG                         GGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
genomic: TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
EST: gi|16926488|gb|BM079556.1|BM079556
EST:     TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAG                         GGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
genomic: TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
EST: gi|211537569|gb|FL441998.1|FL441998
EST:     TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAG                         GGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
genomic: TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
EST: gi|45846842|gb|CN070785.1|CN070785
EST:     TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAG                         GGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG
genomic: TGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 43 (downstream exon)
Score: 22

GGACGTTTACACTATCCAATTTGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 81

GGACGTTTACACTATCCAATTTGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 51

GGACGTTTACACTATCCAATTTGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG


Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 116

GGACGTTTACACTATCCAATTTGGGTATGTTTGGGGTGGATAAATTCGACGCAATCCTTCCTGCTGGTCAGgtgggtttct ... ttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gatagaattgtattctattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG
 tgttgat  putative branch site (score: 116)
     aattgtattctatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgggtttcttccctggtgtatgttgtctgtccagttagtatgaaatgttgatagaattgtatgggtttcttccctggtgtctgatgtttgtctagttagtatgaaatgttgatagaattgtattctattttattaaacacaagaagattgcatggttatatgcagGGGGCTATCATGGCTGTTGGAGCGTCAAGACCTACTGTAGTGGCTGATAAGGATGGTTTCTTTAGTATCAAGAGTGAAATGCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ATGCTG