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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG

Basic information

species Zea mays
transcript GRMZM2G094309_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os05g38150.1 (Oryza sativa), 3'ss of exon 11
gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
||| |  |   ||  |||    ||  | |  |      ||   | |||  | |||||| | ||||||||| | |||||| ||||||| ||||||||||||||||||||||| || |||||||| || || ||||||||||| ||||||||||| ||||||||||| |||||||
gtgtgtatgcaattctat----attttcaaagcgttgatactaccaatttggctaaacccgtaatacactgtgcagCTTGATGATGTCATTGATCTTGTCATTCCAAGAGGAAGTAATAAGCTTGTCTCTCAAATCAAGGCGTCAACTAAGATTCCTGTTCTTGGGCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA01G24530.1 (Glycine max), 3'ss of exon 13
-gtgagcgtaatatattatgaaaatgatgattgta-ccattatgcc-taataagcctaaactcataatacactcta-cagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
  ||    | |  || ||  |||   ||  |  ||   ||| |     |||        ||| || |||| || |  |||||| |||||||||| ||||| ||  | || ||||| || |||||||| ||||| |||||||||| |||||| || || |||||||||||||||||||
gttggatatcac-tagtaaaaaataaatcctcataattattgttttacaatggtttgtcacttattatactctgtctcagCTTGATGATGTAATAGATCTAGTGGTCCCTAGAGGCAGTAATAAGCTTGTTTCTCAAATCAAGGAATCAACAAAAATTCCTGTTCTTGGTCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA18G03830.1 (Glycine max), 3'ss of exon 12
---------------gtgagcgta-atatattatgaaaatgatga--ttgtaccattatgccta----ataagcctaaactcataatacactctac----agCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
                |   | |  ||   ||||     || | |  ||| ||  |  | |||     | ||||   |  |   | |  ||| | |    || |  |||||||||||||||| || ||||||||||| ||||| || || ||  | || ||||||   || ||||| || ||||| || |||||||| |
gtgaatacaaagtagatattcatttatgatttatagtcctgctcactttgaacattggtacctttaacaaaagctagagtttgcattctacttttctttcagTTGGATGATGTAATTGATCTAGTGATTCCAAGAGGCAGCAACAAACTTGTCACTCAGATCAAGAGTTCCACTAAAATTCCTGTCCTGGGTCATGCAG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA02G41850.1 (Glycine max), 3'ss of exon 9
--------gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
         | || | |   || | | |   | || |     |  ||   | |||  |     | ||    ||  |   | |||||  |||||||||| ||||| || ||||||||||| ||||| || || ||||| || ||||||   || ||||| || |||||| | ||||||||||
gtgaactaatcagtggagcttaatttcagtttcataacc---ctgttcaccttaagcattggcacctttctatttaaatttcagCTGGATGATGTAATCGATCTGGTGATTCCAAGAGGCAGCAACAAACTTGTTTCTCAGATCAAGAGTTCCACTAAAATTCCTGTTTTAGGTCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA03G12240.1 (Glycine max), 3'ss of exon 12
-gtgagcgtaatatattatgaaaatgatgattgta-ccattatgcc-taataagcctaaactcataatacactcta-cagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
  ||    | |  || ||  |||   ||  |   |   ||| |     ||| |      ||| || |||| || |  |||||| |||||||||| ||||| ||  | || ||||| || |||||||| ||||| |||||||||| |||||| || || |||||||||||||||||||
gttggatatcac-tagtaaaaaataaatcctcaaaattattgttttacaatgatttgtcactaattatactctgtctcagCTTGATGATGTAATAGATCTAGTGGTCCCTAGAGGCAGTAATAAGCTTGTTTCTCAAATCAAGGAATCAACAAAAATTCCTGTTCTTGGTCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA18G40770.1 (Glycine max), 3'ss of exon 13
gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacact-ctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
                 | ||| |  | |   |    || |    ||| |   | |||| || ||||  | | ||||||| |||| || || ||||||||  | || ||||| || || || || ||||| ||||||||||  || || ||||| |||||||||||||||||||
----------------gtaaaattcctaaa-atttatttgtctgaaatgattttcaactaattatactttgccacagcttgatgacgtgatagATCTTGTGGTCCCCAGAGGCAGTAACAAACTTGTTTCTCAAATCAAGGATTCCACAAAGATTCCTGTTCTTGGTCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA14G07120.1 (Glycine max), 3'ss of exon 9
--------gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
         | || | |   || | | ||  | || |   |       |     ||  |||     | |  |   |   | |||||  |||| ||||||||||| || ||||||||||| ||||| || || ||||| |||||||||   || ||||| || |||||| | ||||| ||||
gtgaactaatcagtggagcttaatttcaatttcataagcctgttcactttaagcattggccacctttaaatacttaaatttcagCTGGATGACGTAATTGATCTGGTGATTCCAAGAGGCAGCAACAAACTTGTTTCTCAAATCAAGAGTTCCACTAAAATTCCTGTTTTAGGTCACGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA07G16510.1 (Glycine max), 3'ss of exon 9
gtgagcgtaatatattatgaaaatgatgat-tgtaccattatgcctaataagcctaaactcataatacact-ctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
                 | ||| |  | |  | ||  | | ||   ||| |   |  ||| || ||||  | | ||||||| |||| || || ||||||||  | || ||||| || |||||||| || || ||||||||||   |||| ||||| |||||||||||||||||||
----------------gtaaaattcctaaaatttattagtctga--aatgattttccactaatcatactttgccacagcttgatgacgtgatagATCTTGTGGTCCCCAGAGGCAGTAATAAGCTTGTCTCTCAAATCAAGGGTACAACAAAGATTCCTGTTCTTGGTCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: AT3G55610.2 (Arabidopsis thaliana), 3'ss of exon 12
gtgagcgtaatatat-tatgaaaatgatgattgtaccattatgcctaa-----------taagcctaaactcataat--acactct----acagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
|| |||   ||| ||  ||   ||||   | |   |     ||  ||            ||  |   ||| ||  ||  ||| | |    ||||||| |||| || ||||||||||| || |||||||| ||||| ||||| ||||| ||||| ||    || || || ||||| || || || |||||||
gtaagca-gatagatacatatgaatggcaagtccccagaagtgattagcttttttcacttatactggaacccaccatttacatttttggtacagCTTGATGACGTTATTGATCTTGTGATCCCAAGAGGCAGCAACAAGCTTGTTTCTCAAATAAAAAACTCGACGAAAATCCCAGTGCTAGGCCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: Vv08s0007g01060.t01 (Vitis vinifera), 3'ss of exon 12
gtgagcgt-aatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacact--ctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
||| |  |  | ||   ||     ||||| || |  ||| ||   |  |     | ||||  |  ||      |  |||||| ||||||| || |||||||| || || ||||| ||||| || || ||||| ||||||||||  |||||||| ||||||||||||||||||||||
gtggggattgagattggatatgtttgatggtt-tttcataataggt--tggttattaacttgttttagttctgccccagCTTGATGATGTCATAGATCTTGTGATCCCTAGAGGCAGCAACAAACTCGTTTCTCAAATCAAGGATTCAACTAAAATCCCTGTTCTTGGTCATGCTG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: EFJ17662 (Selaginella moellendorffii), 3'ss of exon 12
gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
                          | | | |||  |  |||    | ||| |    || ||  |     | |||||| | ||||| ||||| || || || |||||||| |||||||| || ||||||||||||||||  || || || || ||||| || || ||||| |
-------------------------gtaactatactttcgtgct--gttagc-tcgtttcttaccatgggtttcagCTTGACGATGTTATTGACCTCGTTATACCAAGAGGAAGCAATAAACTTGTTTCACAAATCAAGGAGTCTACGAAAATTCCTGTGCTGGGCCATGCCG

upper sequence: GRMZM2G094309_T01 (Zea mays), 3'ss of exon 4
lower sequence: EFJ29127 (Selaginella moellendorffii), 3'ss of exon 12
gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
                          | | | |||  || |||    | ||| |    || ||  |     | |||||| | ||||| ||||| || || || |||||||| |||||||| || ||||||||||||||||  || || || || ||||| || || ||||| |
-------------------------gtaactatacttttgtgct--gttagc-tcgtttcttaccatgggtttcagCTTGACGATGTTATTGACCTCGTTATACCAAGAGGAAGCAATAAACTTGTTTCACAAATCAAGGAGTCTACGAAAATTCCTGTGCTGGGCCATGCCG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 65

GTTATAACTGGTGCCATTCCTAGCAACGTGGGTGAAAAACTTATTGGCCTTGTTACAAGTAGAGACGAAATTGCTGATTTACTAAAGgtgagcgtaa ... cactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG


Block sizes: 46 (upstream exon), 46 (downstream exon)
Score: 15

GTTATAACTGGTGCCATTCCTAGCAACGTGGGTGAAAAACTTATTGGCCTTGTTACAAGTAGAGACGAAATTGCTGATTTACTAAAGgtgagcgtaa ... cactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG


Block sizes: 40 (upstream exon), 36 (downstream exon)
Score: 9

GTTATAACTGGTGCCATTCCTAGCAACGTGGGTGAAAAACTTATTGGCCTTGTTACAAGTAGAGACGAAATTGCTGATTTACTAAAGgtgagcgtaa ... cactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 157

GTTATAACTGGTGCCATTCCTAGCAACGTGGGTGAAAAACTTATTGGCCTTGTTACAAGTAGAGACGAAATTGCTGATTTACTAAAGgtgagcgtaa ... cactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG
                             gcctaaa  putative branch site (score: 157)
                                     tcataata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagcgtaatatattatgaaaatgatgattgtaccattatgcctaataagcctaaactcataatacactctacagCTTCATGATGTAATTGATCTTGTCATTCCAAGAGGTAGCAATAAGCTGGTTTCACAAATCAAGGCATCAACTAAGATCCCTGTTCTTGGTCATGCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  ATGCTG