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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaatctaaacatcattgtggctttaacattcagggacggtgtggtctagctgttgaaacattttatcccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG

Basic information

species Zea mays
transcript GRMZM2G022777_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G022777_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g55260.1 (Oryza sativa), 3'ss of exon 4
gtaatctaaacatcattgtggctttaacattcagggacggtgtggtctagctgttgaaacattttatcccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG
|||||  ||   |||       ||  | |   ||| | |      | | ||  ||||   || |  ||    |  ||   |||||||||| ||||||||||| || ||||||||||||||||| ||||||||||||||||| ||||| |||||||||||||||||||| ||||||||||||||||||
gtaattcaagattcacaa-actttcgaaaaataggaaagcacaaatttggccattgac-tatatattcttgcc--ttcatctttcagGGACATTATTCGCGGCAGTCCAGATGTGAAATGGGAAAGCATCAAAGGACTGGAGAATGCCAAACGCCTTCTAAAAGAGGCAGTTGTCATGCCCATAAAG

upper sequence: GRMZM2G022777_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA08G09050.1 (Glycine max), 3'ss of exon 4
-----------------------------------------------------gtaatctaaacatcattgtggctttaacattcagggacgg-tgtggtctagctgttgaaacattttat---cccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG
                                                     |  |  | |      |  |||     |  |||| |   |  | |  |||    ||| ||   |  |||       |||||   | | | |||||||| ||||| || || ||||||||||| ||||| |||||||| ||  | || ||||| |||||  | || |||||||||||||| ||||| || |||
gtaaattttctctcaatctcttcaacacctctgatagaactgatgactacatggagaagttatttgtgtgttggtcaagattttcatgtgtgaatctattctgtaagtttaattttcatatatgtttgtcattgcacatcttagGGATATCATTCGTGGAAGTCCAGATGTGAAGTGGGAAAGCATCAAGGGGTTAGAGAATGCCAAACGTTTACTGAAAGAGGCTGTTGTGATGCCGATTAAG

upper sequence: GRMZM2G022777_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA05G26100.1 (Glycine max), 3'ss of exon 4
-----------------------------------------------------gtaatctaaacatcattgtggctttaacattcagggacggtgtggtctagctgttgaaacattttat---cccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG
                                                     |  |  | |    |||  |||     |  |||| |     | |  |||    ||| ||   | ||||       |||||   | | | |||||||| ||||| || || ||||||||||| ||||| |||||||| ||  | || ||||| |||||  |  |||||||||||||||| ||||| || |||
gtaaattttctctcaatctcttcaataccttggatagaaccaatgactacatggagaaattatttgcatgttggtcaagattttcatgtg-attctattctgtaagttcaattttcttatatgtttgtcattgcacatcttagGGATATCATTCGTGGAAGTCCAGATGTGAAGTGGGAAAGCATCAAGGGGTTAGAGAATGCCAAACGTTTATTAAAAGAGGCTGTTGTGATGCCGATTAAG

upper sequence: GRMZM2G022777_T01 (Zea mays), 3'ss of exon 4
lower sequence: Vv10s0003g03500.t01 (Vitis vinifera), 3'ss of exon 4
-----------------------------------------------------gtaatctaaacatcattgtggctttaacattcagggacggtgtggtctagctgt-tgaaacattttatcccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG
                                                     ||| | |  |  |      |  ||| | ||||    |     |     |  ||| |   |   ||  || | | ||| | |  | ||||||||| ||||| ||||| ||||||||||| ||||| |||||||| ||  | || ||||| ||||| || || ||||| || ||||||||||| ||||||
gtaattgaacttcttactttggacctgtaatatatctgttatttttgcaatctgtattgtgcatgtggccaggattttcatattctaacagaagatctgaaaattgtataccatgcttcctcgcgttatt-gcctgtccagGGATATCATTCGTGGGAGTCCAGATGTGAAGTGGGACAGCATCAAGGGTTTAGAGAATGCCAAACGTCTGCTTAAAGAAGCAGTTGTCATGCCAATAAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148995361|gb|EE034575.2|EE034575
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|91876817|gb|EB406774.1|EB406774
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|148933119|gb|EC876754.2|EC876754
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|31355560|gb|CD439917.1|CD439917
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|76933170|gb|DV173349.1|DV173349
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|211165539|gb|FL456882.1|FL456882
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|78109695|gb|DV528111.1|DV528111
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|149080059|gb|EE169077.2|EE169077
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|110487038|gb|EE012240.1|EE012240
EST:     CTTCATTTGAGTCAGCTGAAATGCGTAATCTCCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CTTCATTTGAGTCAGCTGAAATGCGTAATCTC-GCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|76280583|gb|DV020151.1|DV020151
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|211131749|gb|FL468282.1|FL468282
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|30705707|gb|CD058888.1|CD058888
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|211305004|gb|FL154239.1|FL154239
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAA
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAA
EST: gi|149106054|gb|EE291707.2|EE291707
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|211307018|gb|FL154240.1|FL154240
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAA
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAA
EST: gi|71297616|gb|DR784759.1|DR784759
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|211305000|gb|FL154235.1|FL154235
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAG
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAG
EST: gi|148963213|gb|EE014191.2|EE014191
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|78121929|gb|DV540313.1|DV540313
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|78106806|gb|DV525224.1|DV525224
EST:     CCTTCATTTGAGTCAGCCGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|76281419|gb|DV020987.1|DV020987
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|67021064|gb|CO449813.1|CO449813
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|211307022|gb|FL154244.1|FL154244
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCC-AGATGTGAAATGGGAGAGCATC
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATC
EST: gi|149035101|gb|EE155087.2|EE155087
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|8367772|gb|BE050717.1|BE050717
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|148963079|gb|EE014036.2|EE014036
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|30307202|gb|CD001875.1|CD001875
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|22472534|gb|BU037014.1|BU037014
EST:     CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
EST: gi|31355569|gb|CD439926.1|CD439926
EST:     CAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAG                         GGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT
genomic: CAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 298

CCAGAAGCCCCTTCTACCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 201

CCAGAAGCCCCTTCTACCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 246

CCAGAAGCCCCTTCTACCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 223

CCAGAAGCCCCTTCTACCTTCATTTGAGTCAGCTGAAATGCGTAATCTCGCAGAGACATTGTTGAGgtaatctaaa ... ggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agggacggtgtggtctagctgttgaaacattttatcccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG
                                               cctttc  CT-rich tract
                     ttgaaacattttat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaatctaaacatcattgtggctttaacattcagggacggtgtggtctagctgttgaaacattttatcccatcatttggcctttcagGGATATTATTCGCGGGAGCCCAGATGTGAAATGGGAGAGCATCAAAGGACTGGAAAATGCAAAACGCCTTCTAAAAGAGGCTGTTGTCATGCCCATAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGTT