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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttagaaatgtgaatgatctttttaattctttaccaatttttatttgttatatatgctatgaaactaccgttcatgtgttctgaaattttattatggtttgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG

Basic information

species Zea mays
transcript GRMZM2G019999_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G019999_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os07g36470.1 (Oryza sativa), 3'ss of exon 2
gttagaaatgtgaatgatctttttaattctttaccaatttttatttgttatatatgctatgaaactaccgttcatgtgttctgaaattttattatggtttgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG
||   |  || |||||  ||  || |||||  |||   ||| || |  ||| |    ||  ||||||  ||||| | |||||||| ||| |  ||| | |||||||||||||||||||||||| ||||| | ||| ||| |||||||| |||| ||||| ||  |||||||||||||||| |  ||||||||  | ||||||||||| |
gtataacctgcgaatggcctaatttattct--acc--ctttcatatactatgt----tacaaaactagtgttcaagggttctgaactttgagaatgttgtgttttgcagCTGAGGAATCCTACCCACCCAAACTGTTCTGTTCCTACTGCTATGCATTGTCTTGCCACTTTGCTAAGAGAGCAGTATGTTAGAGCTTTATTTGTTCAGG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60401227|gb|DN234034.1|DN234034
EST:     CTTCAACACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAG                         CTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGC
genomic: CTTCAACACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGC
EST: gi|6031414|gb|AW076316.1|AW076316
EST:     GATTGGCTCTGTTCACAG                         CTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGC
genomic: GATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGC
EST: gi|6194754|gb|AW146858.1|AW146858
EST:     ACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAG                         CTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGC
genomic: ACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 200

GAATGGCACACTTCCAAATGGAGATGCTTCGAATTCGAAGAGCAAGCTTACTTCAACACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 269

GAATGGCACACTTCCAAATGGAGATGCTTCGAATTCGAAGAGCAAGCTTACTTCAACACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 203

GAATGGCACACTTCCAAATGGAGATGCTTCGAATTCGAAGAGCAAGCTTACTTCAACACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 279

GAATGGCACACTTCCAAATGGAGATGCTTCGAATTCGAAGAGCAAGCTTACTTCAACACACGATGTATTGAGAGGTTTGGTTGATTGGCTCTGTTCACAGgttagaaatg ... tgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgctatgaaactaccgttcatgtgttctgaaattttattatggtttgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG
                                           tttgtttt  CT-rich tract
                             aaattttattatggtt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttagaaatgtgaatgatctttttaattctttaccaatttttatttgttatatatgctatgaaactaccgttcatgtgttctgaaattttattatggtttgttttgcagCTGAGGAATCCTACACACCCGAGCTGCTCTATTCCTACTTCTATCCATTGCCTCTCCACTTTGCTAAGAGAACCATATGTTAGGACATTATTTGTTCAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGA