Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   		6  
 5'  3'   		7  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT

Basic information

species Zea mays
transcript GRMZM2G017386_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g44110.1 (Oryza sativa), 3'ss of exon 5
gtaagtttcaa-----tcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
||||||||||      ||| | ||| |||| |||  |  | |||||   |||    |  ||| | |   |   | ||       |||| |||||     ||| || |||||||||||||||||||| || ||||||||||| || |||||||||||||||||||| || ||||||||||| |||||||||||||| || |||
gtaagtttcatcagcttctcagtctataagatac--atgttgtttctgttgtcggaatttgtttgatgata--ttttggcgggttaatcatgat---cacagACTAGCTTACCTTCATGAAGCTATAGAACCAAAAGTTGTGCACCGAGATATAAAATCAAGCAATATCTTGATTGATGAGGAATTTAATGGCAAGGTCTCT

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA14G03290.1 (Glycine max), 3'ss of exon 3
---gtaagtttcaat--cttaatctgtaagttacctacatagtttc--ctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
   |||  ||  | |  |||| || ||  |||     || || |||     ||    | | | |    | |     |||| | | ||  | ||  |||| |||||||||||||  | |||||||| || || ||||||||| | || || |||||||| || |||||||||||||||||||||||||| ||||  || || |||
ccagtagcttagagtagcttagtc-gtctgttcgtgtcaaagattcaatgatggaccttgttctcctgattaaccccttagtgtactg-tgat--tggtggcagGCTTGCTTATTTACATGAAGCAATAGAACCAAAAGTTATTCACCGGGATATAAAGTCTAGCAACATATTGATTGATGATGAATTCAATGCAAAGGTTTCT

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA02G45540.1 (Glycine max), 3'ss of exon 5
gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttctt-------atttttctaat----tatga-tggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
          || ||| |   | ||||||  |  || |||  ||     ||    | ||  || | |||||        |     |||||    | ||| |||| |||||||||||||  | |||||||| || || ||||||||| | || || |||||||| || ||||||||||||||||| |||||||| ||||  || || |||
--------atattttacttaattagttacaaatttatttttatggaagattcaacg-atggaacttgttctgctaattaacccccctaatgtactgtgactggtggcagGCTTGCTTATTTACATGAAGCAATAGAACCAAAAGTTATTCACCGGGATATAAAGTCTAGCAACATATTGATTGACGATGAATTCAATGCAAAGGTTTCT

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA08G42170.2 (Glycine max), 3'ss of exon 3
---gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttattttt-ctaattatga-tggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
   |   | ||||   |  ||   ||| ||||    || |     |     ||  ||   ||   | || ||    |  ||  | | ||||| |||| ||||||| |||||| | |||||||| || || || ||||| || || || |||||||| |||||||||||||||||||||  ||| || ||||  || || |||
tgtgcttgcttca--ttggatacctaatttactgttatggcagtttcaacattaggcctaattccactgattaaactccttatttactatgactggtggcagGCTAGCTTACTTACATGAAGCAATAGAACCGAAAGTCGTTCACCGGGATATAAAGTCAAGCAACATATTGATTGATACTGATTTCAATGCAAAGGTTTCT

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA18G12830.1 (Glycine max), 3'ss of exon 3
--gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttattttt-ctaattatga-tggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT-
  |   | ||||  ||  ||   ||| ||||    || |     |     ||  ||   ||   | || ||    |  ||  | | ||||| |||| ||||||| |||||| | |||||||| || || || |||||||| || || |||||||| || ||||||||||||||||||  ||| || ||||  || || ||| 
gtgcttgcttca--ctggatacctaatttactgttatggcagtttcaacattaggcctaattccactgattaaactccttatttactatgactggtggcaggCTAGCTTACTTACATGAAGCAATAGAACCGAAAGTTGTTCACCGGGATATAAAGTCTAGCAACATATTGATTGATACTGAGTTCAATGCAAAGGTTTCTG

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: AT2G42960.1 (Arabidopsis thaliana), 3'ss of exon 3
gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
||||||     | | || | ||        |      | |  |   |  ||   | | |||||  |  |   |||| |  || |       || ||||||||||||| | |||||||| || || |||||||| || ||  |||| |||||| |||| || ||||||||||||||||| |||||||  ||| | |||
gtaagt---ggtttgaaacagtttagcttttgttggatatgttcaatgcttgattat-taaaagggtctgcaatttgtacaact-------ttacagGCTTGCTTACTTGCATGAAGCAATAGAACCAAAAGTGGTCCACAGAGACATAAAAGCAAGTAATATATTGATTGATGATGAGTTTAATGCAAAACTCTCT

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: Vv12s0028g00310.t01 (Vitis vinifera), 3'ss of exon 5
--------gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
          |    | ||   || ||   || | |  |    |  |    || |||    || |  |||| |       || ||||  |||     |||||||||||||| ||  | ||||| || || || || |||||||| |||||||| ||||||||||||||||| |||||||||||||| || ||||  || || |||
tattccattcagaactgaaatctactccagaactgaaat-tgaacatcttggtctttcacactttgaaaaagtcaaaaaaatgtttcatattg----ggttgcagGCTTGCCTATTTGCATGAGGCAATAGAACCGAAAGTTGTTCATCGAGACATAAAATCAAGCAACATCTTGATTGATGATGAGTTCAATGCAAAGGTTTCT

upper sequence: GRMZM2G017386_T03 (Zea mays), 3'ss of exon 4
lower sequence: EFJ16857 (Selaginella moellendorffii), 3'ss of exon 4
gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
             | | ||| |   |||  |  ||    ||          || |    | |  ||  | | |||    |  || |    ||  |||| | || || || || |||||| | ||||| |||||||||||| | || |||||||||||||||||| | ||||||      |  || | ||   |||| 
------------gtaagtctttgccttatgtttat----tcgaccaccccgagttactcacagctg--tttgattcgattgcttctctgcgtgccagGTTAGCATATCTGCACGAAGCTTTGGAGCCCAAAGTTGTACATAGGGACATAAAATCAAGCAACATACTCATTGATTCAACTTACAACGCCAGGATATCA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|5901151|gb|AW042251.1|AW042251
EST:     AGGCACTGCAAAAGC                         GCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATAT
genomic: AGGCACTGCAAAAGCgtaagtttca ... atggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATAT




















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 192

ACAATGGTAATTTAGAGCAGTGGCTTCATGGAGCTATGCATCAGCGTGGTGTTTTTAGCTGGGAAAATCGCATGAAGGTTGTAACAGGCACTGCAAAAGCgtaagtttca ... atggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 102

ACAATGGTAATTTAGAGCAGTGGCTTCATGGAGCTATGCATCAGCGTGGTGTTTTTAGCTGGGAAAATCGCATGAAGGTTGTAACAGGCACTGCAAAAGCgtaagtttca ... atggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 54

ACAATGGTAATTTAGAGCAGTGGCTTCATGGAGCTATGCATCAGCGTGGTGTTTTTAGCTGGGAAAATCGCATGAAGGTTGTAACAGGCACTGCAAAAGCgtaagtttca ... atggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 116

ACAATGGTAATTTAGAGCAGTGGCTTCATGGAGCTATGCATCAGCGTGGTGTTTTTAGCTGGGAAAATCGCATGAAGGTTGTAACAGGCACTGCAAAAGCgtaagtttca ... atggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT
                                  ttctaat  putative branch site (score: 116)
              tataaaattggttctt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtttcaatcttaatctgtaagttacctacatagtttcctgtgtttttagctgtataaaattggttcttatttttctaattatgatggttgcagGCTTGCTTACCTTCATGAAGCTATTGAGCCAAAAGTTGTACATCGAGATATAAAATCAAGCAACATATTGATTGATGATGAATTTAATGGCAAAGTATCT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG