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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtagttaattttttggagagccaaatcgtatgttggaatgatgtttgttttagccaaatcatatgcctcatatctgatcagacctttttttttctttccatccaataaactggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG

Basic information

species Zea mays
transcript GRMZM2G014376_T01
intron # 4
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78091464|gb|DV519838.1|DV519838
EST:     TTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAG                         TTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGA
genomic: TTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGA
EST: gi|6626287|gb|AW257819.1|AW257819
EST:     TTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAG                         TTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGA
genomic: TTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGA
EST: gi|32929488|gb|CF034300.1|CF034300
EST:     TTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAG                         TTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGA
genomic: TTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 110

TCAGATCTGTGGGTTTCAACGCTGTGTATGTGCCATTTTTGGTGGATGACTTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 94

TCAGATCTGTGGGTTTCAACGCTGTGTATGTGCCATTTTTGGTGGATGACTTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 105

TCAGATCTGTGGGTTTCAACGCTGTGTATGTGCCATTTTTGGTGGATGACTTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 136

TCAGATCTGTGGGTTTCAACGCTGTGTATGTGCCATTTTTGGTGGATGACTTGGCTAAATTTCTTGATACATACTCTTCACCAGACTTTGCTGGCTTCAGgtagttaatt ... tggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cctcatatctgatcagacctttttttttctttccatccaataaactggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG
      atctgat  putative branch site (score: 136)
                   tttttttttcttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtagttaattttttggagagccaaatcgtatgttggaatgatgtttgttttagccaaatcatatgcctcatatctgatcagacctttttttttctttccatccaataaactggcccacagTTGCACAATTCCACACAAAGAAGCTGCTGTTAGGTGCTGTGACGAGGTCGATCCCGTTGCCAGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT