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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG

Basic information

species Zea mays
transcript AC218972.3_FGT001
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: AC218972.3_FGT001 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os01g60440.1 (Oryza sativa), 3'ss of exon 4
--------taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
        ||  |    | | |   ||  | |||||  |  | || |||    | |  ||    |||| |  | ||||       ||| ||||| |  ||   |  ||||||| || ||||||||| || | || || |||||||| ||||| || ||||||||||||||||||||||||||||| || |||||||||||||| || |
catgttcttatttgtgcaaagtcatgtaatgacacacgaaaacaatgtat---gttggaagttctttggtcataaaatt-tgaaattgtcatca-tcaat---ttcagGTTCACGGGAATGTATGCGTGACGAGCGTAGTTGTTACTCAAACTCTAGATTGGAAGCTTCATGCTTTTGATGTTCTTTCTGAATTTGATGCTAACAACG

upper sequence: AC218972.3_FGT001 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA20G36180.1 (Glycine max), 3'ss of exon 4
taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataa--acgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
             || |   | |  |  ||  || |||      |  |||    || | ||| || |    |    | | |  ||    |||     |  |||||||||||||||| |||||||| ||||||||||| ||||||||  |||| ||||| || ||||||||||||||||| ||||| ||||| |  | |||||
------------gtgagtgttcatgagctagatataaatatggtctttatggtcactagttgcttttttggt--tttttctaacaaactttgcgtgttgcagGTTCATGGTAATGTTTGCTTGGCTAGTGTTGTTGTCACACAAACTTTGGACTGGAAACTCCATGCTTTTGATGTTCTATCAGAGTTTGAAGGGAGTAATG

upper sequence: AC218972.3_FGT001 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA18G47590.1 (Glycine max), 3'ss of exon 4
taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
             || |   |||  |  |  ||      |   |   | ||      ||  || | ||||       ||| ||    ||      |  |||||||||| || || ||| |||| ||||| ||||| |||| |||  |||| |||||||||||||||||||||||||| ||||| |||||||  |  | ||
------------gtgagtatttatgagcttttttattgtttttattttgtattattattattttttaaatatatc--ttgacaagttttatctgttgcagGTTCATGGCAACGTTTGCATGGCTAGTGTCGTTGTGACACCAACTTTGGACTGGAAGCTTCATGCTTTTGATGTTCTATCAGAGTTTGATGGGAGCAGTG

upper sequence: AC218972.3_FGT001 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA09G38740.1 (Glycine max), 3'ss of exon 4
taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
             || |   |||  |  |  ||      |   |   | |       ||  || | ||||       ||| |||   ||      |  |||||||||| ||  | ||| |||| |||  |||||| |||| |||  |||| ||||||||||||||||| |||||||| || || |||||||  |  | ||
------------gtgagtatttatgagcttttttattgtttttattttgttttattgttattttttaaatatatc--ttgacatgttttatctgttgcagGTTCATGGCAACATTTGCATGGCTAGTACTGTTGTCACACCAACTTTGGACTGGAAGCTTCATGCTTTGGATGTTCTATCGGAGTTTGATGGGAGCAGTG

upper sequence: AC218972.3_FGT001 (Zea mays), 3'ss of exon 4
lower sequence: AT2G40730.1 (Arabidopsis thaliana), 3'ss of exon 4
-taaat-caaaacaatggggtcttaacaca-taattagaaagtacaac-gcta--taaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
 ||| |        ||       |||  ||   ||||||   || ||| | |   ||  ||||||  | || |||   | |   | | |     | |     | |||| ||||||||||| ||  |||| ||||||||||||||||  ||  |||| ||||| || |||||| |||||||||| ||||| |||||||  |  ||||
gtaagtatctgtttataacactataattcagctattagagtatataaccggtggctagtcgattttttagttaaaactcttaagttctactgtctta-----acagGTGCATGGTAATGTCTGTCTGGCAAGTGTTGTTGTTACACCTACTTTGGACTGGAAACTCCATGCTCTTGATGTTCTATCAGAGTTTGATGGGAGCAATG

upper sequence: AC218972.3_FGT001 (Zea mays), 3'ss of exon 4
lower sequence: Vv13s0067g03110.t01 (Vitis vinifera), 3'ss of exon 4
taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataaacgatttgattgtcaaatgac-ctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
|    |       |        |  | | || |   || |  |  | |      ||| | || ||   |||   || | || |     |||     || |||||||||||||||| |||||||||||||||||||| || ||||| ||||| |||||||| ||||||||||| ||||| || || |||||||   |||  |
tgctcctctggtgtcacacaccacaaaacaagttagaaatctattggtgcctctgatatcat-gttggatgttactttctgacgagcattggcttctacagGTTCATGGTAATGTTTGCTTGGCCAGTGTTGTTGTCACTCAAACTCTGGACTGGAAGCTGCATGCTTTTGACGTTCTATCTGAGTTTGATGGCCATAGCG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32906847|gb|CF011660.1|CF011660
EST:     TTCATCAGGTATCCAAAGCTGTGAGCTTTCTCAATAATGACTGCAAACTT                         GTTCATGGTAATGTATGCTTGACCAGTGTTGTTGTTACACAAACCCTGGAT
genomic: TTCATCAGATATCCAAAGCTGTGAGCTTTCTCAATAATGACTGCAAACTTgtaagttcct ... atcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGAT




















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 251

AGATGAGTACTTTGCATGGGGGCTTCATCAGATATCCAAAGCTGTGAGCTTTCTCAATAATGACTGCAAACTTgtaagttcct ... atcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 176

AGATGAGTACTTTGCATGGGGGCTTCATCAGATATCCAAAGCTGTGAGCTTTCTCAATAATGACTGCAAACTTgtaagttcct ... atcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 140

AGATGAGTACTTTGCATGGGGGCTTCATCAGATATCCAAAGCTGTGAGCTTTCTCAATAATGACTGCAAACTTgtaagttcct ... atcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 202

AGATGAGTACTTTGCATGGGGGCTTCATCAGATATCCAAAGCTGTGAGCTTTCTCAATAATGACTGCAAACTTgtaagttcct ... atcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gctataaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG
                                        acttgat  putative branch site (score: 202)
                                             atcaatata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
taaatcaaaacaatggggtcttaacacataattagaaagtacaacgctataaacgatttgattgtcaaatgacctgtttgccatcacttgatcaatatagGTTCATGGTAATGTATGCTTGGCCAGTGTTGTTGTTACACAAACCCTGGATTGGAAGCTTCATGCTTTTGATGTTCTGTCAGAATTTGATGCTAATAATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAAG
- - - aaaacaa
- - - - - - - - - - - aacacat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttgattg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ccatcac