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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttccttggtctgtcttaaaatctcaaggatacactatattccagtacggaaggagtagatccatgcacctgctatcactaataatcaagtcctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG

Basic information

species Zea mays
transcript GRMZM5G816609_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G816609_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os02g05340.1 (Oryza sativa), 3'ss of exon 17
-ttccttggtctgtcttaaaatctcaaggatacactatattccagtacggaaggagtagatccatgcacctgctatcactaataatcaagtcctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG
  | ||    ||| |||    ||| |  | |    |||       | | |      |||||  ||| ||||  ||| | |  |   ||| | | ||||||||| ||||| |||||||| ||||| || ||||||||||||||||| |||||| | |||||||||||||||||||||||||| || |||||||| || ||||
tatactatagctgcctttgtttctaactgttgt-ttatggctttatgcagcccttttagataaatgtaccttttatgattgtttggcaatttccatttcagGAGAGTGTAGAGGCTACGGCAGAAGTTTCTAAAACATTTGATGAGAAGATAAGAAAGTACTGTGATGTAACACTTATGTCACTAGCATATGCTGGAACAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32929745|gb|CF034557.1|CF034557
EST:     TTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAG                         GATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATA
genomic: TTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATA
EST: gi|32911849|gb|CF016661.1|CF016661
EST:     GTTGCGCTTGGCCTTCTATACCTTGGAAAACAG                         GATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATA
genomic: GTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATA
EST: gi|32935986|gb|CF040798.1|CF040798
EST:     TTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAG                         GATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAGGATA
genomic: TTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATA
EST: gi|67041940|gb|CO468195.1|CO468195
EST:     ATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAATTTTG                         GACACTGTGGAGGCTACCGCATAGGTCTCTAAAACATTTGATGAAAAGATA
genomic: ATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 224

TATTATATTTGCCTTGATGGAGCGTAGTGAGGCTGAGTTGGCAGAGCCCATTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 192

TATTATATTTGCCTTGATGGAGCGTAGTGAGGCTGAGTTGGCAGAGCCCATTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 176

TATTATATTTGCCTTGATGGAGCGTAGTGAGGCTGAGTTGGCAGAGCCCATTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 159

TATTATATTTGCCTTGATGGAGCGTAGTGAGGCTGAGTTGGCAGAGCCCATTATACGGTTGCTTCCTGTTGCGCTTGGCCTTCTATACCTTGGAAAACAGgtaattctaa ... cctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

acggaaggagtagatccatgcacctgctatcactaataatcaagtcctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG
                              cactaat  putative branch site (score: 159)
                                            tcctatttc  putative PPT
                                 taataatcaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttccttggtctgtcttaaaatctcaaggatacactatattccagtacggaaggagtagatccatgcacctgctatcactaataatcaagtcctatttcagGATAGTGTGGAGGCTACCGCAGAGGTCTCTAAAACATTTGATGAAAAGATAGGGAAGTACTGTGATGTAACACTTATGTCGCTGGCATATGCCGGGACAG

- - - - - - - - - - - - - -gatacac
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tccatgc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -cctgcta