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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttatgcttgtaaccacaaaaattctgagaagaatatctgcaagcatcatctaatcaatccttgtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG

Basic information

species Zea mays
transcript GRMZM2G550865_T01
intron # 3
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67042557|gb|CO468812.1|CO468812
EST:     AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAG                         CCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
genomic: AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
EST: gi|19058457|gb|BM737124.1|BM737124
EST:     AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAG                         CCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGG
genomic: AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
EST: gi|60337607|gb|DN204580.1|DN204580
EST:     AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAG                         CCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
genomic: AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
EST: gi|87152018|gb|DY396807.1|DY396807
EST:     AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAG                         CCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
genomic: AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
EST: gi|18963625|gb|BM660540.1|BM660540
EST:     AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAG                         CCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT
genomic: AAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 388

CATCAAGATCTTAGCAAATGCTGATGGACTTCGGTTGCTGCGCACGCTGGAAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 264

CATCAAGATCTTAGCAAATGCTGATGGACTTCGGTTGCTGCGCACGCTGGAAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 295

CATCAAGATCTTAGCAAATGCTGATGGACTTCGGTTGCTGCGCACGCTGGAAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 330

CATCAAGATCTTAGCAAATGCTGATGGACTTCGGTTGCTGCGCACGCTGGAAAATCGTTCTTTTGATGCTTCTCGTAATGCATCTGAGGCTGTAACAAAGgttatgcttg ... gtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aaaattctgagaagaatatctgcaagcatcatctaatcaatccttgtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG
                              atctaat  putative branch site (score: 330)
                                        tccttgttt  putative PPT
           aagaatat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttatgcttgtaaccacaaaaattctgagaagaatatctgcaagcatcatctaatcaatccttgtttatgcagCCTCTAATAAATCCCTTGACTGCTGCGGCAAATGCAGCTGCAGCAAGCAGTTCAGGAGCTGGTGCTCCGTCAGCTGTAACTGCAATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG