ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatattatatgatcaacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT

Basic information

species	Zea mays
transcript	GRMZM2G469523_T01
intron #	3
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os08g28730.1 (Oryza sativa), 3'ss of exon 8

gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatattatatgatcaacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT
||||||  || ||| | | |||||       |||||    |    ||| |||  |   |  | |      | ||||| | | ||||| ||||  || || || || |||||  | || |||||||||||||| ||||||||||||||||| || || |||| |||||| | ||||||||| || 
gtaagttttacatcaacggataatca-----catga----acctttttcttggtttccacctaactggtattgtctttccccagAGCGAAATTTGGCATCGATGAAATGTGTAGGGATCAATGGAACTGGGCTAGCAAGAACCCTTATGGATACGGGTCACTTGACTCCACCAAGCAGAACGGA

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA08G02690.2 (Glycine max), 3'ss of exon 7

---------------gtaagtcatatatcgaggtataatatgttatc--atgacaaaatggctttattgtatattatatgatcaacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGA---CTCTATCAAGCAGAATGGT
               |  |   ||| ||  || |     | ||| ||  ||    | ||    |   | | | || | | | ||| ||  |  || |  ||  | |||||  | || || || |||||||| || |||||||| ||||| || || |||||||||||||||||   | ||||    ||   |||      |   
atattttcatcttttgataaaaataaataaagttgggcgaggttctctaattgttatatcctctctctttttcttttctcaccaa-tttattgtcgtgcagGACTAAATATTGCATCGATGATATGTGCCGAGATCAATGGAATTGGGCAAGGAAAAACCCTTATGGCTATGGTGGATCTGAAGATTCCTCCAATTGATGT---

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA11G01940.2 (Glycine max), 3'ss of exon 5

-----gtaagt-catatatcgaggtataatatgttatcatgacaaaatggcttt--attgtatattatatgatc------aacttcgtcttcctc--tagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGA--TCACCTGACTCTATCAAGCAGAATGGT
     |||  | |||  ||     | ||| |  || |  |    |  |   ||   ||| | | || | |  |       || ||| |||| |    ||| |||||||| || ||||| |||||||||||||| |||||||| ||||| ||||| ||||||||||||||||||  |||   |  |    |      | |   
ttgtagtactttcatgcattttactttaaaaaattcttttattcacttcttttccaattttgtttttttttctttctcaaaatttcatcttacgtggtagGGCAAAATATGGCATTGATGAGATGTGCCGCGATCAATGGAATTGGGCTAGCAAAAACCCTTATGGCTATGGAGATCAGGAGGATTCCACCGTTTAACTA--

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA05G36850.1 (Glycine max), 3'ss of exon 8

----------------gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatattatatgat-caacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTG---ACTCTATCAAGCAGAATGGT
                 ||| | | | |  | || |   | | |     ||  |     |||   || |   || | |    ||| ||  ||  | |  ||  | |||||  | || || || ||||||||||| |||||||| ||||| ||||| |||||||||||||||||  || |||   | ||   |||      |   
atgttttcgtcttctgataaataaaaaaatg-ggcaaggtttctctaattgttatttccgctccctttttctttttttctcacaaatttatcgccgtgcagGACTAAATATAGCATCGATGATATGTGCCGCGATCAATGGAATTGGGCTAGCAAAAACCCTTATGGCTATGGTGCATCTGCAGATTCCTCCAATTGATGT---

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA01G43540.2 (Glycine max), 3'ss of exon 7

-----------------------------------------------------------------------------------------------------------gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatattatatgatcaacttcgtcttcctc--tagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT
                                                                                                            |   |  ||  |  |   |   | | ||  | |           ||| || | | ||   |    || ||| |||| |     || |||||||| || ||||| ||||||||||| || |||||||| ||||| ||||| ||||||||||||||||||   |  | ||| | | |  |      |
gtaagtgttaattggctcctcctcctgaatatcctagatagataataaaagcttaatagtatgaaacaagtttttcttgataaattctggttttgtgtagtactttcatgcattttacttttaaaaaacttttattcacttcttttc-caattttgttttttttttcttctaaaaattcatcttacgtggcagGGCAAAATATGGCATTGATGAGATGTGCCGTGATCAATGGAATTGGGCTAGCAAAAACCCTTATGGCTATGGAGATCAGGGCTCCACCGATTAACCACTT

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: Vv02s0025g01580.t01 (Vitis vinifera), 3'ss of exon 8

-----------------gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatattatat-gatcaacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT-
                 | |  |    | |  |  | |    ||| | ||| |||  |    |   |  ||| | |  |  ||     |  | | |||  || || || || || ||    |||||||| || ||||||||||| ||||||||||||||||| |||||||| | |||  |   |   |  |  |  || | | 
aattagaacataagtgggaagatttggtttgaaaatcttggttgtaaccat-acatga-aaatgagtcttatgtcacttcaatttgtgtgatgtccctgcagGGCCAAGTATGGCATCAGCGAGATGTGTCGGGACCAATGGAATTGGGCCAGCAAGAACCCCTATGGCTACGAATCGTCCC-CAACACAAGACTAAAGGCTG

upper sequence: GRMZM2G469523_T01 (Zea mays), 3'ss of exon 3
lower sequence: Vv02s0025g01560.t01 (Vitis vinifera), 3'ss of exon 8

------------gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatatt-atatgatcaacttcgtc---ttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT
            | ||||| |   |  | || |||     ||  |||    ||     | | || | ||  | || ||    || ||    | |||  || ||||| ||||| ||    |||||||| || ||||| ||||||||||||||||||||||| |||||||| | |||| |     | |   |  | ||    
tgattttgtttggaaagtcttggttgtacgtgtaaccatgtaagatgtgggaaaaatatcaatgaacatggaaatcattctttttgtgatgtccctgcagGGCAAAGTACGGGATCAGTGAGATGTGTCGGGACCAGTGGAACTGGGCCAGCAAGAACCCATATGGCTACGAATCATCCCCAACAACAGACTAAAACATC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|60397877|gb|DN230693.1|DN230693

EST:     TTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: TTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|211051082|gb|FL157953.1|FL157953

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|32837892|gb|CD977570.1|CD977570

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGT
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGT

EST: gi|60352711|gb|DN219684.1|DN219684

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|24764028|gb|CA399197.1|CA399197

EST:     GTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|71773386|gb|DR971290.1|DR971290

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|67025401|gb|CO454150.1|CO454150

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|24774129|gb|CA409383.1|CA409383

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|4646708|gb|AI491571.2|AI491571

EST:     GAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|67025022|gb|CO453771.1|CO453771

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|60358489|gb|DN225462.1|DN225462

EST:     AAGCAGAGAGGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: AAGCAGAGA-GGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|71425858|gb|DR806940.1|DR806940

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|148997319|gb|EE035814.2|EE035814

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|31361907|gb|CD446264.1|CD446264

EST:     TTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: TTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|32846391|gb|CD986072.1|CD986072

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTACCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|50326912|gb|CO522038.1|CO522038

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|31357216|gb|CD441573.1|CD441573

EST:     GAGATTTTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|24773701|gb|CA408955.1|CA408955

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|5739820|gb|AI947510.1|AI947510

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|16378594|gb|BI992508.1|BI992508

EST:     CTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: CTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|33468176|gb|CF245225.1|CF245225

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|60354871|gb|DN221844.1|DN221844

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|83278826|gb|DV942834.1|DV942834

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|60396877|gb|DN229696.1|DN229696

EST:     AGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: AGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

EST: gi|74245905|gb|DT653819.1|DT653819

EST:     GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAA                         AGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG
genomic: GAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 831

AAAATACCTCTGATTTTTGGTGAAAGACGCCCAGGTGATGCAGAGATTCTGTTTTCAGAGACTACTAAAGCAGAGAGGGAGCTTAACTGGAAgtaagtcata ... cttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 920

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 609

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1149

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

atcatgacaaaatggctttattgtatattatatgatcaacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT

                                       cttcgtcttcctct  CT-rich tract
                tttattgtatattata  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtcatatatcgaggtataatatgttatcatgacaaaatggctttattgtatattatatgatcaacttcgtcttcctctagAGCAAAATACGGTATTGAAGAGATGTGCCGCGACCAATGGAACTGGGCCAGCAAGAACCCTTATGGCTATGGATCACCTGACTCTATCAAGCAGAATGGT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAC