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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaggttacatgcatcccttgtccatactaaatccacgcttattcaaatagatgttgtttcaaacgtgaatactgaagtgaatgttctgtattatttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT

Basic information

species Zea mays
transcript GRMZM2G421791_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G421791_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os05g45490.1 (Oryza sativa), 3'ss of exon 3
gtaggttacatgcatcc-cttgtccatactaaatccacg-cttattcaaatagatgttgtttcaaacgtgaatactgaagtgaatgttctgtattatttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT
||||||||| ||||||  |||  ||||| | ||||||   ||| | | || |  |  ||  | ||| ||| || |||||   |||| | |||  | ||| |||||||||||||||||||||||| |||||||||||| ||||||| |||||||| |||||||||||| ||||||||||| ||||||||||||| || |||||||||
gtaggttacttgcatcttcttacccatatttaatccagaacttctgccaaaacttaatggctgaaatgtgtatgctgaatgaaatgatttgtgctgttt-tatcagCTTTGTTAGAGGAAAGAAACCTGGCCAAGGTTCTGGATTTGATATCAATGATGATGATCTGTTAGAGATGGAGAAATGCCGTGAGCTGGTTGTAGCTTCT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|22521602|gb|BU080413.1|BU080413
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|71439406|gb|DR820456.1|DR820456
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|21760101|gb|BQ635642.1|BQ635642
EST:     ATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: ATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|26559312|gb|CA831547.1|CA831547
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|149105943|gb|EE291571.2|EE291571
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|148987277|gb|EE031113.2|EE031113
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|87156073|gb|DY400862.1|DY400862
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|149097873|gb|EE183807.2|EE183807
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
EST: gi|149087531|gb|EE176929.2|EE176929
EST:     CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGG                         CTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA
genomic: CAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 39 (upstream exon), 45 (downstream exon)
Score: 26

CAAATTTATCAAATCTAGGATCTGGATTTGGAGGGCATCCATCTCTGGAGCAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT


Block sizes: 46 (upstream exon), 43 (downstream exon)
Score: 41

CAAATTTATCAAATCTAGGATCTGGATTTGGAGGGCATCCATCTCTGGAGCAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 100

CAAATTTATCAAATCTAGGATCTGGATTTGGAGGGCATCCATCTCTGGAGCAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 149

CAAATTTATCAAATCTAGGATCTGGATTTGGAGGGCATCCATCTCTGGAGCAGAGAAACAAATCTTTCAACATTAATGAGTCAATGACTGTCCACTGTGGgtaggttaca ... tttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agatgttgtttcaaacgtgaatactgaagtgaatgttctgtattatttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT
                                          ttatttct  CT-rich tract
                                      tgtattatttctat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaggttacatgcatcccttgtccatactaaatccacgcttattcaaatagatgttgtttcaaacgtgaatactgaagtgaatgttctgtattatttctatcagCTTTGTTAGAGGAAAGAAGCCTGGCCAAGGTACTGGATTCGATATCAAGGATGATGATCTGCTAGAGATGGAGCAATGCCGTGAGCTAGTCGTAGCTTCT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GATGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGCA