ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtagtttactattattgcaggcataggactataggaggtgttccatatgcacatgtacttctcatacaacaaaattgcgagaaatctgtagatcaccctgcttcaacagctaaatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA

Basic information

species	Zea mays
transcript	GRMZM2G323936_T01
intron #	3
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G323936_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os12g34840.1 (Oryza sativa), 3'ss of exon 4

gtagtttactattattgcag----gcataggactataggaggtgttccatatgcacatgtacttctcatacaacaaaattgcgagaaatctgtagatcaccctgcttcaacagctaaatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA
||| | | | |||  || |     |||  |||  |   ||   ||     || |    ||||||  | ||||| | ||    ||     |||  | |  | ||               |||   |||||  |||||||| ||||||||||||||  ||||||| || |||||||| ||||||||||||||||| ||||||||| ||||||||||||| ||||| ||||| || |||||||| |
gtaatct-ccatttctgtaattttgcaggggatcaggtgatacgtaggtgattcttcagtactttccctacaaaagaacc-tga-----ctgaggtttgctct---------------tttg--tttttgggttctatgctagATGTCAGATCCTTTGACAGCCCTAATGCATGCTGTCCAAGTCATGAACTTCCTCAAGACATTGATCTTGAGGACACTTCGAGAGCGTGACGATGCAGCTA

upper sequence: GRMZM2G323936_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA10G02720.1 (Glycine max), 3'ss of exon 3

gtagtttactattattgcaggcataggactataggaggtgttccatatgcacatgtacttctcatacaacaaaattgcgagaaatctgtagatcaccctgcttcaacagctaaatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA
                            | |||    |  | |  || ||  ||  |  |     |||  |||| |  |   | | || | ||         || |  ||||| |   | | | | | | ||     ||||||| ||||| || ||||| || |||||||| || ||||| |||||||| ||||||||||| ||| ||| ||| ||| | || | |||  |  |||| |
---------------------------gccatattttgcttacagtaggc-tatagatcttatgcacatgaaaactatgtatagt-tgaaaatgg-------tctattgctaatt---catctgtgtctcctggaacagATGTCTGATCCTCTAACAGCCCTGATGCATGCGGTTCAAGTGATGAACTTACTCAAGACACTCATCATGAAGACACTTAGAGAACACGAACAAACAGCAA

upper sequence: GRMZM2G323936_T01 (Zea mays), 3'ss of exon 3
lower sequence: PP1S60_256V6.1 (Physcomitrella patens), 3'ss of exon 4

--------------------------------gtagtttactattattgcaggcataggactataggaggt--gttccatatgca--catgtacttctcatacaacaaaattgcg---agaaatctgtagatcaccctgcttcaacagct------------aaatttact----tttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA
                                | || | |  |  ||| || |   ||     |||    |    | || ||||   | |  || | ||||      || |||     ||  ||     | | |   || |||||  | |            | ||||| |    ||  ||  || |   | ||||| | |||||||||||||| || |||||||| || |||||||||||||| ||||| || |||||  |  | || ||   ||| ||  | |   |     
gtatagtgttgtttctacgcttttcacccagagaagctgatgaccattacaagatcagactcgtagagatttcactacagatgctgtcttcgacgtttcatgtgctgaatttgaacgtaggtattccgtgtttatggtgtttcaattgttgtagaatgtgtaacatttattgacattgcttcattatccttcagATGGCTGATCCACTGACAGCTCTGATGCATGCTGTTCAAGTCATGAACTTGCTCAAAACTCTGATTCTTCGCACACTGAAGGACCGAAAGGCCACTTTGC

upper sequence: GRMZM2G323936_T01 (Zea mays), 3'ss of exon 3
lower sequence: EFJ22564 (Selaginella moellendorffii), 3'ss of exon 4

gtagtttactattattgcaggcataggactataggaggtgttccatatgcacatgtacttctcatacaacaaaattgcgagaaatctgtagatcaccctgcttcaacagctaaatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA
                                                     |    | ||| | ||       |||  |   | |||        |||            ||| || ||  |||||  || |   ||||| | |||||||| |||||||||||||| || || ||||| |||||| |||| ||||| |||||  ||||||| || |||||  |  | || || |   
----------------------------------------------------gtacgttcctctttcatttgg--tgcttggtttttgttttcttttctgt-----------aatgtatttccttttg--ctgtcccagATGGCGGATCCACTAACAGCACTCATGCACGCTGTACAAGTGATGAACATTCTAAAGACGCTGATTGTGAGGACACTACGGGACAGGCAAGAGGCTGTG-

upper sequence: GRMZM2G323936_T01 (Zea mays), 3'ss of exon 3
lower sequence: EFJ12967 (Selaginella moellendorffii), 3'ss of exon 4

gtagtttactattattgcaggcataggactataggaggtgttccatatgcacatgtacttctcatacaacaaaattgcgagaaatctgtagatcaccctgcttcaacagcta-aatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA
                                                                |||       || |      |   | | |     || ||      ||  ||| || ||  |||||  || |   ||||| | |||||||| |||||||||||||| || || ||||| |||||| |||| ||||| |||||  |||||||||| |||||  |  | || || |   
---------------------------------------------------------------gtacgttcctctttcatttggttcttggtt---tttgttttgttttctgtaatgtatttccttttg--ctgtcccagATGGCGGATCCACTAACAGCACTCATGCACGCTGTACAAGTGATGAACATTCTAAAGACGCTGATTGTGAGGACGCTACGGGACAGGCAAGAGGCTGTG-

upper sequence: GRMZM2G323936_T01 (Zea mays), 3'ss of exon 3
lower sequence: EFJ18297 (Selaginella moellendorffii), 3'ss of exon 4

gtagtttactattattgcaggcataggactataggaggtgttccatatgcacatgtacttctcatacaacaaaattgcgagaaatctgtagatcaccctgcttcaacagctaaatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA
                            || | ||  || ||||     ||  |  | ||  ||      | |||| |      |  |    ||||  ||  ||| |   | ||||   ||| || || ||  | | ||||| |||||||  ||||||| |||||||| ||||||||||| |||||  | ||||| ||  ||||| |  ||||| |||| ||  |  | ||        
---------------------------gcttttgggtgtcttcc-----caagtaga-tttgcaagtgctagaatttctgttccttcgcttctcac--tgtctcatctctttaatt---tttattctgctccgt-tcagATGGCAGATCCGTTGACAGCGCTCATGCACGCCGTCCAAGTGATGAATCTCCTCAAAACGTTGATCCTCCGGACGATTCGAGACAGGCAAGAG-------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|78091661|gb|DV520035.1|DV520035

EST:     GAACGTAGCCATGGTGTTTGCCCCCAACATGACACAG                         ATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT
genomic: GAACGTAGCCATGGTGTTTGCCCCCAACATGACACAGgtagtttact ... tctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT

EST: gi|50323991|gb|CO519117.1|CO519117

EST:     AGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAG                         ATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT
genomic: AGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAGgtagtttact ... tctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT

EST: gi|78092422|gb|DV520796.1|DV520796

EST:     AGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAG                         ATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT
genomic: AGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAGgtagtttact ... tctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT

EST: gi|78091775|gb|DV520149.1|DV520149

EST:     CCCCCAACATGACACAG                         ATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT
genomic: CCCCCAACATGACACAGgtagtttact ... tctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT

EST: gi|9732144|gb|BE510896.1|BE510896

EST:     AGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAG                         ATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT
genomic: AGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAGgtagtttact ... tctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 143

TTGGGCTGTCGAGCTGATGTCTGATGTTGTCGAAGAGGAGGAGCTCAACAAGATGAATGCTAGGAACGTAGCCATGGTGTTTGCCCCCAACATGACACAGgtagtttact ... tctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA

Block sizes: 28 (upstream exon), 47 (downstream exon)
Score: 5

Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 14

Block sizes: 47 (upstream exon), 46 (downstream exon)
Score: 10

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tctgtagatcaccctgcttcaacagctaaatttacttttttttgttctatgttagATGTCAGATCCACTGACAGCACTCATGCATGCCGTCCAAGTCATGAACTTTCTCAAGACACTGATCTTGAGGACGCTTCGGGAGCGCGATGATGCAGCCA