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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtccgaactgaacctgtgttcttataattttcagtctattttttttaccttcagacctcaaagctacagtagggtaacatccttaagtatttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAGAG

Basic information

species Zea mays
transcript GRMZM2G314386_T02
intron # 3
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211311912|gb|FL092760.1|FL092760
EST:     CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGAT                         GAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAG
genomic: CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAG
EST: gi|148970703|gb|EE021421.2|EE021421
EST:     CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGAT                         GAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATC
genomic: CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATC
EST: gi|211311913|gb|FL092761.1|FL092761
EST:     CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGAT                         GAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAG
genomic: CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAG
EST: gi|211311911|gb|FL092759.1|FL092759
EST:     CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGAT                         GAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAG
genomic: CTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 3 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 30 (downstream exon)
Score: 2

TTCCATCTGGACAGCAAATCTTGGTGAAGAATTTTCTCTTGATTCCCTCCCTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAGAG


Block sizes: 18 (upstream exon), 49 (downstream exon)
Score: 4

TTCCATCTGGACAGCAAATCTTGGTGAAGAATTTTCTCTTGATTCCCTCCCTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAGAG


Block sizes: 49 (upstream exon), 15 (downstream exon)
Score: 9

TTCCATCTGGACAGCAAATCTTGGTGAAGAATTTTCTCTTGATTCCCTCCCTCATTTAGACCGGGTGGCTGCCCTCGTTATGGTCTTCGATATGAGTGATgtccgaactg ... atttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttaccttcagacctcaaagctacagtagggtaacatccttaagtatttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAGAG
                            gggtaac  putative branch site (score: 9)
                                       ttaagtatttat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtccgaactgaacctgtgttcttataattttcagtctattttttttaccttcagacctcaaagctacagtagggtaacatccttaagtatttatgcagGAATCCACCTTGCTTACTCTGCAAAGCTGGGCTGCCAGTGTTGATATCCAGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCTG