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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgtttgaaactaacatttagtttctgtctgtaagtggaatactgaatagtagtaccattgattgttttatgttgtttaagctttttctttattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG

Basic information

species Zea mays
transcript GRMZM2G167999_T02
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G167999_T02 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os12g06810.1 (Oryza sativa), 3'ss of exon 3
--ttgtttgaaactaacatttagtttctgtctgtaagtggaatactgaatagtagtaccattgattgttttatgttgtttaagctt-tttctttattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG
  |  |     |  |   || || ||   | |||||   | |  ||   |  |  |  | |  | |  ||| | |  ||||||||  | | |||||  |||||||||||||||||||||| |||||  | || ||||||||||| |||||||| || ||  |||||
actcatccttgaggagatttgagcttaattttgtaacatggacgctt--tcattatctc-tgaagtactttctatgatttaagctgatatatttatcatgcagGTTAATGATGCCATTATTGTTGGCCAAAATCTTGTTTCCTGTTCCTCAGATACCACTCTAAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211344544|gb|FL091812.1|FL091812
EST:     ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGG                         GTTAATGATGCCATTATAGT
genomic: ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGT
EST: gi|22489370|gb|BU049293.1|BU049293
EST:     ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGG                         GTTAATGATGCCATTATAGNTGGAGACAAGCTTGTTTCCTGCTCCTCAGAC
genomic: ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGAC
EST: gi|88758366|gb|DY542735.1|DY542735
EST:     ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGG                         GTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGAC
genomic: ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGAC
EST: gi|60397544|gb|DN230360.1|DN230360
EST:     ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGG                         GTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGAC
genomic: ATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 203

TCATCTTTTTAGCGGGAGCCGTGATGGTACCTTGAAAAGATGGGAATTAAATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 195

TCATCTTTTTAGCGGGAGCCGTGATGGTACCTTGAAAAGATGGGAATTAAATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 174

TCATCTTTTTAGCGGGAGCCGTGATGGTACCTTGAAAAGATGGGAATTAAATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 338

TCATCTTTTTAGCGGGAGCCGTGATGGTACCTTGAAAAGATGGGAATTAAATAATGGGGATGCAAGCTTCTCAGGAACTTTCGAGTCTCATGTTGATTGGgtatgctttt ... tattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aatagtagtaccattgattgttttatgttgtttaagctttttctttattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG
           cattgat  putative branch site (score: 338)
                                    ctttttctttattgt  putative PPT
                    ttttatgttgtttaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttgtttgaaactaacatttagtttctgtctgtaagtggaatactgaatagtagtaccattgattgttttatgttgtttaagctttttctttattgtgcagGTTAATGATGCCATTATAGTTGGAGACAAGCTTGTTTCCTGCTCCTCAGACACTACAATAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTCCTC
ttgtttg
- - - -aaactaa
- - - - - - - - - - - - ctgtctg
- - - - - - - - - - - - - - - - - - - - -actgaat
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - ttgattg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgttgtt