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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gctgtttgttctcatgtccttgcttagaaaccctgagtcaagtcttagttggatatgttcactaacatcagcttttacaatatgttctctcatattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC

Basic information

species Zea mays
transcript GRMZM2G126656_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G126656_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os12g07190.1 (Oryza sativa), 3'ss of exon 3
--gctgtttgtt-ctcat-gtccttgcttagaaaccctgagtcaagtcttagttggatatgttcactaacatcagcttttacaatatgttctctcatattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC
   |  |||||| | ||| | ||||| || |    |  ||    ||||    ||  | |     ||| ||   || || |  || ||||||   ||||||||||| |||||||||||||| || ||||| ||||||||||||||||||||||||||||||||||| ||||||||||| || |||||| ||||||||||||||||
tttcactttgttacacatagaccttggttgg----ctagaaattagtccctttttcacaaacatactgactgtagtttctttaaaatgttcatacatattttagGTAAATTTAGACACCTCCCAGTTGTGGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCTAAATGCCTTTACGATGCAATATCAAGACTGGAAAAGGC

upper sequence: GRMZM2G126656_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA06G16570.2 (Glycine max), 3'ss of exon 3
--gctgtttgttctcatgtccttgcttagaaaccctgagtcaagtcttagttggatat--gttcactaacatcagcttttacaatatgttctctcatattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC
    | | | || | ||     |  || || |    || |||     | | |||| |    |||| |||        | ||   ||  || | | |  |  |||| | |||||||||||| || ||||| ||||||||||| || ||||| |||||||| ||  | ||||| |||||||| || ||| | ||| |||| |  ||
ggattctctatttttatacatcttgtttgata----gaatcatagtctggctggaaagtaattcattaagtgagagatataatttaactttttttaactgatagGTAGATTTAGACACCTCCCTGTTGTGGAAAATGGTGAAGTCATTGCCATGCTGGATATCACCAAATGTCTTTATGATGCCATATCTAGAATGGAGAGCGC

upper sequence: GRMZM2G126656_T01 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA04G38480.1 (Glycine max), 3'ss of exon 3
gctgtttgttctcatgtccttgcttagaaaccctg---agtcaagtcttagttggatatgttcactaa--catcagcttt----tacaatatgttctctcatattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC
        |  |    ||  || ||| | | | ||   | |  |||| |||| |||  ||   |||||  ||| |  |      || ||| ||   | | |  |  |||| ||||| |||||||| || || || ||||||||||| || ||||| |||||||| ||| | |||||||||||||| || ||| | ||  |||| |  ||
--------tattagattctctgtttatacatcttgtttaatagagtcatagtcggac-tggaaactaattcattaagtgagagatataatttgactttttaactgatagGTAAATTCAGACACCTCCCTGTCGTGGAAAATGGTGAAGTCATTGCCATGCTGGATATTACCAAATGCCTTTATGATGCCATATCTAGGATGGAGAGCGC

upper sequence: GRMZM2G126656_T01 (Zea mays), 3'ss of exon 3
lower sequence: Vv07s0031g02750.t01 (Vitis vinifera), 3'ss of exon 3
--gctgtttgttctcatgtccttgcttagaaaccctgagtcaagtcttagttggatatgttcactaacatcagcttttacaatatgttctctcatattt-----tagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC
    | |||  | |||| |     |||        ||| | || ||||  ||   ||| |   |||| ||  | |||||    || ||  ||  | ||       ||| || ||||| ||||| || ||||||||||||||||| |||||||| ||  |||| ||| |||| ||||| ||||| || ||| |  || |||| |||||
tatttatttcatttcatatatgcacttca----tttgaatgaattctttattacttat-tagcctaatatttg-ttttatggcatcttg-cttgtgttggtcaacagGCAAGTTTAGGCACCTCCCTGTTGTAGAAAATGGTGAAGTTATTGCCATATTGGATATTACAAAGTGCCTCTATGATGCTATATCTCGAATGGAGAAGGC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71764856|gb|DR962793.1|DR962793
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|71304764|gb|DR788496.1|DR788496
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|211362912|gb|FL253415.1|FL253415
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGNTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|78078047|gb|DV506462.1|DV506462
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|78086694|gb|DV515087.1|DV515087
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|30704520|gb|CD058548.1|CD058548
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|71766059|gb|DR963996.1|DR963996
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
EST: gi|148949419|gb|EC894878.2|EC894878
EST:     TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAG                         GGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC
genomic: TGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 49

AGGGTGGAGCAAACCATCATGTCCAAGATCATGACACGAAATCCTGTTTATGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 50

AGGGTGGAGCAAACCATCATGTCCAAGATCATGACACGAAATCCTGTTTATGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 39

AGGGTGGAGCAAACCATCATGTCCAAGATCATGACACGAAATCCTGTTTATGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 29

AGGGTGGAGCAAACCATCATGTCCAAGATCATGACACGAAATCCTGTTTATGTGATGTCTGACACGCTCGCCATTGAGGCTTTGCAGAAGATGGTTCAAGgtataactag ... catattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tagttggatatgttcactaacatcagcttttacaatatgttctctcatattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC
              cactaac  putative branch site (score: 29)
                                       ttctctcatatttt  putative PPT
                           ttttacaatatgtt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gctgtttgttctcatgtccttgcttagaaaccctgagtcaagtcttagttggatatgttcactaacatcagcttttacaatatgttctctcatattttagGGAAATTTAGACACCTTCCCGTTGTAGAAAATGGTGAGGTTATTGCTATGCTGGACATTGCAAAATGCCTTTATGAAGCAATAGCAAGACTGGAAAAGGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAGA
- tgtttgt
- - - - - - - - tccttgc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -catcagc