ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

gtatatatacacactgacgattgtgaacaacgcaatggtctcaatttctactcacacggccggccgcggcctctcgttttcgtgtcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTCAAGGTGGAGGTCCTCGTGGTCATTGGCCATAGCTGCTGCGGTGGCATCA

Basic information

species	Zea mays
transcript	GRMZM2G121878_T04
intron #	3
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G121878_T04 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os01g45274.2 (Oryza sativa), 3'ss of exon 5

-----------------------gtatatatacacactgacgattg-tgaacaacgcaatggtctcaatttctactcacacggccggccgcggcctctc----gttttcgtgtcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTCAAGGTGGAGGTCCTCGTGGTCATTGGCCATAGCTGCTGCGGTGGCATCA
                        |   |   |   | | |||||| | | | |   | |    ||  |  |  |          |     | || | |    || |  | ||| |||||||| |||| || | ||| ||||||| |||||||||||||| || ||||| ||||||                                              
gtaaccaacttcatcgtacgcgttttcgtcgccggccggtcgattggtaagctagctattccggtcggtcgccgcggtttgcattgattttgacccccctcgtgtgtgtgcgtcaactgcagATCAAGCACGCTGGCGTCGGGTCGGCCATCGAGTACGCCGTCTGCGCCCTCAAG----------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|13558048|gb|BG549404.1|BG549404

EST:     AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|5525295|gb|AI861134.1|AI861134

EST:     AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|8318768|gb|BE025333.1|BE025333

EST:     AGGCCTTCACCGTTCGCAACATCGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCCCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|5525294|gb|AI861133.1|AI861133

EST:     AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|8318769|gb|BE025334.1|BE025334

EST:     AGGCCTTCACCGTTCGCAACATCGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCCCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|13515934|gb|BG518210.1|BG518210

EST:     AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGCTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCT
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAG-TACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCT

EST: gi|7031284|gb|AW461022.1|AW461022

EST:     AGGCCTTCACCGTTCGCAACATCGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCCCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|211520600|gb|FL477904.1|FL477904

EST:     CAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCCCTC
genomic: CAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|10697521|gb|BE997245.1|BE997245

EST:     AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|13558507|gb|BG549863.1|BG549863

EST:     AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

EST: gi|6827008|gb|AW330651.1|AW330651

EST:     AGGCCTTCACCGTTCGCAACATCGCCGCCATGGTCCCAGGCTACGACAAG                         ACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCCCTC
genomic: AGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 3 RNA-Seq libraries.

Block sizes: 40 (upstream exon), 48 (downstream exon)
Score: 2

CTCCGACTCCCGTGTGTGCCCATCGGTGACCCTGGGCCTGCAGCCCGGCGAGGCCTTCACCGTTCGCAACATAGCCGCCATGGTCCCAGGCTACGACAAGgtatatatac ... tcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTCAAGGTGGAGGTCCTCGTGGTCATTGGCCATAGCTGCTGCGGTGGCATCA

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 211

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 67

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ctcaatttctactcacacggccggccgcggcctctcgttttcgtgtcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTCAAGGTGGAGGTCCTCGTGGTCATTGGCCATAGCTGCTGCGGTGGCATCA

         tactcac  putative branch site (score: 67)

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatatatacacactgacgattgtgaacaacgcaatggtctcaatttctactcacacggccggccgcggcctctcgttttcgtgtcgactgcagACCAAGTACACCGGCATCGGGTCCGCCATCGAGTACGCTGTGTGCGCTCTCAAGGTGGAGGTCCTCGTGGTCATTGGCCATAGCTGCTGCGGTGGCATCA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC