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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatataccgcatcagcagaaagtccttagctaaattcaacctgcttgtattttctaggtctaatgatcgtaaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA

Basic information

species Zea mays
transcript GRMZM2G099547_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G099547_T03 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os07g19470.1 (Oryza sativa), 3'ss of exon 3
--------------gtatataccgcatcagcagaaagtccttagctaaattcaacctgct--tgtattttctaggtcta-atgatcgtaaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA
                ||   ||   | | |   |  || ||||     || |  ||  |  |  || ||||| |   | |  |   | |||  ||||||||| |||||||| ||||||||||||||| |||||||||| |||||||||||||||||||| || ||||||||||||||||| |||||||||||||
gtattaccaatttaccatgagccatgtgaactata--tcattagtcct-tttagtctattattccatgttctaagctaacaacactttgaaatggcagGATGCACCTGTACCACCTTGCATCACACAATTGGAAGGTGGCCTTGTCCAAGTAGCCATTGAGGTTGAAGACCGGGCACAACGGTCAGCAATAACAA

upper sequence: GRMZM2G099547_T03 (Zea mays), 3'ss of exon 3
lower sequence: AT5G63440.3 (Arabidopsis thaliana), 3'ss of exon 3
gtatataccgcatcagcagaaagtccttagct--aaattcaacctgcttgtattttctaggtctaatgatcgtaaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA
||| | |  |  |    | ||  |  | |  |  ||  ||||      |  ||     |  | |  ||   | |      |||||||| || |||||||| |||||  ||||||| || ||||||||||| || || || || |||||||||||||| ||||||||||||||||| ||||
gtaca-attggtttcttataatcttttcaattccaagatcaaa--aactagatcactaatctgttgtggcggca----cacagGATGCACCAGTACCTCCCTGCATTTCACAACTCGACGGTGGACTTGTTCAGGTTGCAATAGAAGTGGAAGACCGTGCACAACGTTCAGCAATCACAA

upper sequence: GRMZM2G099547_T03 (Zea mays), 3'ss of exon 3
lower sequence: Vv17s0000g01530.t01 (Vitis vinifera), 3'ss of exon 3
----------------gtatata--ccgcatcagcaga----aagtccttagctaaattcaacctgcttgtattttctaggtctaatgatcgtaaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA
                |||  |   | |||||          || | ||    ||  |  || |||     ||||  |  |     ||||       ||  ||||||||||||| |||||||||||  ||||  | ||||| || ||||||||||| || || || ||||||||||| |||||||| || ||||| ||||
ttttatacttctatgagtagttgatctgcatctttgttttccaaattctcgaatagttataaactgtggaaatttgttttggactctgatttc---tctctagGATGCTCCTGTGCCTCCTTGCATATCACAGTTAGAAGGGGGGCTTGTCCAAGTGGCAATAGAGGTGGAAGACCGTGCACAACGCTCTGCAATTACAA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|16926773|gb|BM079841.1|BM079841
EST:     GAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: GAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|60394679|gb|DN227549.1|DN227549
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|15199102|gb|BI423657.1|BI423657
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGA
EST: gi|94477735|gb|EB706693.1|EB706693
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGGAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|101392937|gb|EB818399.1|EB818399
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|12045537|gb|BF727676.1|BF727676
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGA
EST: gi|149104413|gb|EE289858.2|EE289858
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|211360252|gb|FL253401.1|FL253401
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|60348216|gb|DN215189.1|DN215189
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|12045916|gb|BF728146.1|BF728146
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGTAATGTGGAGTTGTCCA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGG-AAGGTGGACTTGTCCA
EST: gi|148980593|gb|EE027217.2|EE027217
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|32846204|gb|CD985885.1|CD985885
EST:     ACGTTATTGACGGAGCGCTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|86471527|gb|DY237897.1|DY237897
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|71433975|gb|DR815025.1|DR815025
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|60337078|gb|DN204051.1|DN204051
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|60337543|gb|DN204516.1|DN204516
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|71433974|gb|DR815024.1|DR815024
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGTTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|71450211|gb|DR831261.1|DR831261
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|60351167|gb|DN218140.1|DN218140
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGAGTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|84351516|gb|DW245921.1|DW245921
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGATACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|16920282|gb|BM074585.1|BM074585
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|71757029|gb|DR954966.1|DR954966
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|16925502|gb|BM078570.1|BM078570
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|78180203|gb|DV550576.1|DV550576
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGAGTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|6827273|gb|AW330916.1|AW330916
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGAGTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|19822251|gb|BQ048275.1|BQ048275
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGAGTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|71435787|gb|DR816837.1|DR816837
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCAT
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCAT
EST: gi|78117103|gb|DV535490.1|DV535490
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|78075508|gb|DV503942.1|DV503942
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
EST: gi|50325142|gb|CO520268.1|CO520268
EST:     ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACAT                         GATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA
genomic: ACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 342

TTTTGTTTGTTACCGATCAGAAGAGGATCTGGAGGTTGCCCCTTTCATATACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 279

TTTTGTTTGTTACCGATCAGAAGAGGATCTGGAGGTTGCCCCTTTCATATACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 255

TTTTGTTTGTTACCGATCAGAAGAGGATCTGGAGGTTGCCCCTTTCATATACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 278

TTTTGTTTGTTACCGATCAGAAGAGGATCTGGAGGTTGCCCCTTTCATATACGTTATTGACGGAGCACTGAGTTCAGTTGCTGCTGAGACAAATCCACATgtatataccg ... aaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttagctaaattcaacctgcttgtattttctaggtctaatgatcgtaaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA
                                gtctaat  putative branch site (score: 278)
     taaattcaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatataccgcatcagcagaaagtccttagctaaattcaacctgcttgtattttctaggtctaatgatcgtaaaactgcagGATGCTCCTGTACCTCCTTGCATCACACAACTGGAAGGTGGACTTGTCCAAGTAGCCATTGAAGTGGAAGACCGGGCACAACGTTCAGCAATAACAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAAGA