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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttggccatttacgagaaagtaaagttttgttatgtggttattatgttatgctttgtgaacttttgttcatgtggattgatctttcatgttgtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG

Basic information

species Zea mays
transcript GRMZM2G087144_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G087144_T02 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os09g35960.1 (Oryza sativa), 3'ss of exon 3
-ttggccatttacgagaaagtaaagttttgttatgtggttattatgttatgctttgtgaacttttgttcatgtggattgat-ctttcatgttgtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG
  |||   ||  | | |||||    |    || |   ||| |  | | |||  || ||   |       |    ||||||  || |||  |  ||| |||||||||||||| |||||| | ||||| ||||||||  | ||||| |||||||||||
tatggttgttgtc-ataaagtctgttcggattgttgtgttgt-gtttaatgtattctgtcttcaaaccaagagagattgacgctgtcaattcttatgtgcagCTATGATTTAAATAGCACAGAAGACGAAAGGAATACCATCAAAATCAAGAAAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|50332287|gb|CO527413.1|CO527413
EST:     GGCATTGAAGCAAGCATTACGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|50325323|gb|CO520449.1|CO520449
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|148959376|gb|EC905424.2|EC905424
EST:     GCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCC-TTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|211240371|gb|FL028807.1|FL028807
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|148964953|gb|EE015802.2|EE015802
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|211328212|gb|FL409834.1|FL409834
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTG
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTG
EST: gi|211240372|gb|FL028808.1|FL028808
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|149106092|gb|EE291756.2|EE291756
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
EST: gi|148981030|gb|EE027393.2|EE027393
EST:     GGCATTGAAGCCAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CCATGATTTGAATAGCTCTGAAGAGGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAA
EST: gi|148939166|gb|EC883612.2|EC883612
EST:     GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGT                         CTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA
genomic: GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 87

GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 59

GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 40

GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG


Block sizes: 48 (upstream exon), 45 (downstream exon)
Score: 31

GGCATTGAAGCAAGCATTAGGGCTGAAGTTTGGCCCTTCCTACTAGGAGTgtgagttact ... gtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttatgctttgtgaacttttgttcatgtggattgatctttcatgttgtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG
                            gattgat  putative branch site (score: 31)
            aactttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttggccatttacgagaaagtaaagttttgttatgtggttattatgttatgctttgtgaacttttgttcatgtggattgatctttcatgttgtatctgcagCTATGATTTGAATAGCTCTGAAGAGGAAAGGAACTCTATCAAGATCAAGAAAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGATCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAA
- - - - - - - -aaagtaaa
- - - - - - - - - - - - ttttgtt
- - - - - - - - - - - - - - - - - - - - - - - tatgctt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tcatgtt