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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatgtatctttgtaaattcagttttctattgttgtttggaaattatattgcagacgtgtgattgctccaactggatgctaccttaattatatttactttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG

Basic information

species Zea mays
transcript GRMZM2G051262_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G051262_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os01g11070.1 (Oryza sativa), 3'ss of exon 3
gtatgtatctttgtaaattcagttttctattgttgtttggaaattatattgcagacgtgtgattgctccaactgga-tgctacctta-attatatttactttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG
|||||| |||  |  | ||||||| | |||| |  | |||||  | ||||||||  |||| | |  || ||||| | || ||||||| ||| | ||| | || || |||||||||||||||||||||||||||||||||| ||||||||||| |||||||||||||| |||||||| ||||||| ||||||||| ||||||||||| ||
gtatgtttctcag--atttcagttctatattatcatatggaagctctattgcagctgtgtaactattcaaactgaagtggtaccttatatttttttttccttattgcagGTTTCTTGCCAAGTTCATTCTCCATGTATGCTGTAACACTTTCCTCTGCACTCTTCCTACTTGAGAACTATGCTGCTGCAGTCTCTGTTGCAGCTGCAGG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|28909967|gb|CB331189.1|CB331189
EST:     TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCA                         GTTTCTTGCCAA
genomic: TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAA
EST: gi|148995029|gb|EE034347.2|EE034347
EST:     TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCA                         GTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
genomic: TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
EST: gi|74233286|gb|DT641200.1|DT641200
EST:     TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCA                         GTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
genomic: TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
EST: gi|71772097|gb|DR970034.1|DR970034
EST:     TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCA                         GTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
genomic: TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
EST: gi|91054950|gb|EB165368.1|EB165368
EST:     TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCA                         GTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
genomic: TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
EST: gi|149100385|gb|EE186457.2|EE186457
EST:     TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCA                         GTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC
genomic: TGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 158

GTTCTGGTGGTTGCTCTTTCAAGAAGATATGGGAAGCGGCTTGCTTGTTATGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 79

GTTCTGGTGGTTGCTCTTTCAAGAAGATATGGGAAGCGGCTTGCTTGTTATGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 95

GTTCTGGTGGTTGCTCTTTCAAGAAGATATGGGAAGCGGCTTGCTTGTTATGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 181

GTTCTGGTGGTTGCTCTTTCAAGAAGATATGGGAAGCGGCTTGCTTGTTATGTTCTTGCAATGCTATGCTTATCCAGCGGTTGCTTCTTTGCTAGTACCAgtatgtatct ... tttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

agacgtgtgattgctccaactggatgctaccttaattatatttactttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG
                             ccttaat  putative branch site (score: 181)
                                            ctttgttcc  putative PPT
                               ttaattatatttactt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgtatctttgtaaattcagttttctattgttgtttggaaattatattgcagacgtgtgattgctccaactggatgctaccttaattatatttactttgttccagGTTTCTTGCCAAGTTCATTCTCCATGTATGCAGTAACACTTTCGTCTGCACTCTTCCTTCTTGAGAAATATGCTGGTGCAGTCTCAGTTGCAGCTGCTGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ATGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGCT