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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...atggctttagaaacaatcacagtgtcgttatgtcctgtttctgtgtgatttctgtgaaagtcagatggccaaagctaattaaattatgctctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG

Basic information

species Zea mays
transcript GRMZM2G031435_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G031435_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os05g44910.1 (Oryza sativa), 3'ss of exon 3
-----atggctttagaaacaatcacagtgtcgttatgtcctgtttctgtgtgatttctgtgaaagtcagatggccaaagctaattaaattatgctctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG
      |    || || | |||   |       | ||  |     || || | || |  |  || |  |||      |  |  ||    | |  |  || |||||| |||| |   |||||||||||||| ||||| || |||||||||||||||||||||||||||||||||||||| || | ||||
aattcgtttgattggacagaattgtaaccatcatttgaacaacaactctgag-ttacactagaaattggatttatgcatatgttt----tttttttcttctccagGAAATGCATCACAAGGTGGTAGCACTTATGTACATTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTATGCTATTTG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71441815|gb|DR822865.1|DR822865
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|93284231|gb|EB676495.1|EB676495
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|71448351|gb|DR829401.1|DR829401
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|71439137|gb|DR820187.1|DR820187
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGGTA-GGCTGGAAGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTG-TAAGGCTGGAAGG
EST: gi|71766612|gb|DR964549.1|DR964549
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|19057494|gb|BM736161.1|BM736161
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|78103952|gb|DV522370.1|DV522370
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|71430075|gb|DR811125.1|DR811125
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|76288945|gb|DV028513.1|DV028513
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|76280780|gb|DV020348.1|DV020348
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|87152319|gb|DY397108.1|DY397108
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|74232090|gb|DT640004.1|DT640004
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|101383931|gb|EB814020.1|EB814020
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
EST: gi|22491044|gb|BU050967.1|BU050967
EST:     GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATT                         GTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG
genomic: GTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 47 (downstream exon)
Score: 25

GCTCATGGAATCAATCATCTCAAAATTCCTACAAGAGACTACCTGTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG


Block sizes: 45 (upstream exon), 47 (downstream exon)
Score: 33

GCTCATGGAATCAATCATCTCAAAATTCCTACAAGAGACTACCTGTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 42

GCTCATGGAATCAATCATCTCAAAATTCCTACAAGAGACTACCTGTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 71

GCTCATGGAATCAATCATCTCAAAATTCCTACAAGAGACTACCTGTTTGCCCCATCCCTGGAGCATATTTGTCGTGCAGTGGATTTTATCCATTgtaagtatgt ... ctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgatttctgtgaaagtcagatggccaaagctaattaaattatgctctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG
                           agctaat  putative branch site (score: 71)
                                      ttatgctctttttcc  putative PPT
                              taattaaattatgctc  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
atggctttagaaacaatcacagtgtcgttatgtcctgtttctgtgtgatttctgtgaaagtcagatggccaaagctaattaaattatgctctttttccagGTAATGAAGTGCAAGGTGGTAGCACCTATGTCCACTGTAAGGCTGGAAGGGGACGAAGCACCACTATAGTTTTGTGTTTTTTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAAG
- - - - - aaacaat
- - - - - - - - - - - - - - - - - -tgtttct
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttatgct