ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

gttcacctctctaccctcctacctttctctgcaataacattcttgataaaatttgcaagccttctgacgccctgtttttatttcctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCTGCATTGAGCCGAGATCTACGGGGTACTCATTTACGGGCTCTTCTAGGGG

Basic information

species	Zea mays
transcript	GRMZM2G014066_T01
intron #	3
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G014066_T01 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os01g48190.1 (Oryza sativa), 3'ss of exon 3

gttcacctctctaccctcctacctttctctgcaataacat-------tcttgataaa-----------atttgcaagccttctgacgccctgttt----ttatttcctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCTGCATTGAGCCGAGATCTACGGGGTACTCATTTACGGGCTCTTCTAGGGG
||| | |||  | |   ||||   |  |   |||  | ||         ||||||||           ||||   |   ||||||  || | ||     ||||||   |  ||||||  |||| ||||   ||||||| |  ||||||||||||||| ||| |||||  | ||||||||||||| |||| | |||| |||| ||| |||||||| |
gttaatctccttccatacctattctgttgcacaagcatatgctaattctttgataaattttcttaattatttaggaatgttctgattcctttttcaaaattatttgtgatgtacagAGTGGTCGCAGGATGCGCAGATTCGATATTCCCGAGAGCCAGGCACTTTCTTTACTGAGCCGAGATCTGCGGGATGCTCAGTTACAGGCACTTCTAGGAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|74239572|gb|DT647486.1|DT647486

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|71305275|gb|DR788771.1|DR788771

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|71422365|gb|DR805702.1|DR805702

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|78074874|gb|DV503308.1|DV503308

EST:     CATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|149086120|gb|EE175660.2|EE175660

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|94472198|gb|EB701156.1|EB701156

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|148958296|gb|EC904516.2|EC904516

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|148990777|gb|EE032191.2|EE032191

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|32792051|gb|CD944287.1|CD944287

EST:     TAACCATATGACCGTGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: TAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|18654784|gb|BM499589.1|BM499589

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|71427572|gb|DR808622.1|DR808622

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|20300095|gb|BQ163038.1|BQ163038

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|18963077|gb|BM660226.1|BM660226

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|87152622|gb|DY397411.1|DY397411

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|211484735|gb|FL156869.1|FL156869

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCT

EST: gi|89247987|gb|DY619773.1|DY619773

EST:     AAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTNCA-G                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAG
genomic: AAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTT-CAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAG

EST: gi|76936894|gb|DV174950.1|DV174950

EST:     CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAG                         AACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGNCA-TTTC
genomic: CAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATG-CAATTTC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 57

CCCTGCCCCATTTGCTCCCAAAGGGTTACAAGAGATATGCTTAACCATATGACCATGCAGCATGGTTACCTGTTCAAGgttcacctct ... ctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCTGCATTGAGCCGAGATCTACGGGGTACTCATTTACGGGCTCTTCTAGGGG

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 63

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 78

Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 41

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttcttgataaaatttgcaagccttctgacgccctgtttttatttcctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCTGCATTGAGCCGAGATCTACGGGGTACTCATTTACGGGCTCTTCTAGGGG

                      ttctgac  putative branch site (score: 41)
                              ccctgtttttatttcc  CT-rich tract
                                 tgtttttattt  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttcacctctctaccctcctacctttctctgcaataacattcttgataaaatttgcaagccttctgacgccctgtttttatttcctacttacagAACGGTCACAGGTCTCGCAGATACATTATTCCCGAGAGCCATGCAATTTCTGCATTGAGCCGAGATCTACGGGGTACTCATTTACGGGCTCTTCTAGGGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATC