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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ctccatgtaatgtttagtactatgatgttcatgcctacctcttagatctttctaatttccaatgacagtgttactatattctttttatgtattattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG

Basic information

species Zea mays
transcript AC212112.4_FGT002
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: AC212112.4_FGT002 (Zea mays), 3'ss of exon 3
lower sequence: LOC_Os02g50880.1 (Oryza sativa), 3'ss of exon 3
ctccatgtaatgtttagtactatgatgttcatgcctacct-cttagatctttctaatttccaatgacagtgttactatattctttttatgtatt---attttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG
   ||    ||| |||  |||   || ||  ||      | | |    |       |||   | |     |||| | || | | || |||||||   |||| ||| || |||||||||||| | ||||||||||||||||||||||| |||||||||||||| |||||||||||||| ||||||||||||||||||||| ||||
---cagtctatggttaccacttctatctttctggtattttacatttgccacgtggttttttgaaggttccgttatt-taatatcttcatgtattataatttcagGATTCCCTATACTTGGAATAGAATGGCAGAAAATGGAGAATCCTGATCTCCGCAAAGCGATGGGAATGAAACCTGATCAAAAGGGTGTTCGTGTTCGAAG

upper sequence: AC212112.4_FGT002 (Zea mays), 3'ss of exon 3
lower sequence: GLYMA05G01930.1 (Glycine max), 3'ss of exon 3
ctccatgtaatgtttagta-ctatgatgttcatgcc------------tacctcttagatctttctaatttccaatgaca-gtgttactatatt-ctttttatgtattattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG
    || |  |   ||| | ||| |||||  ||  |            | |  | |     ||| ||   |  | |   | |   |   || || |||| | | | |  | ||||| || ||||| ||||| ||||| |||||||||||||| ||||| ||||| || |  || ||||| |||||||| ||||| || ||||| ||  |||||||
-gtaatatggtagctaggaactaagatgtagatttcacttttacaagatgctacctgagcatttttagaattaagttttatgcactcagatctttctttctctatctgctgttagGATTCCCTATTCTTGGGGTTGAGTGGCAGAAAATGGAAAATCCTGATCTGCGAATGGCCATGGGCATGAAACCTGATCAGAAAGGTGTGCGCATTAGAAG

upper sequence: AC212112.4_FGT002 (Zea mays), 3'ss of exon 3
lower sequence: Vv17s0000g07900.t01 (Vitis vinifera), 3'ss of exon 3
-ctccatgtaatgtttagtactatgatgttcatgcctacctcttagatc-tttctaatttccaatgacagt-gttactatattctttttatgtattattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG
  |   |    || ||  |  | | ||| |||  |||   | ||       |||||||| |  |||| | | ||||  ||  | || || |  | | ||  ||| || || || ||  |  |||| |||||||| ||||| ||||| ||| | || |  ||||||||||||||||| ||||| ||||| ||||||||||||||
aatttttcccttgctttatgatgtaatgctcaaaccttttttttcccctgcttctaattcc--atgatatttgttatcatgattttgtttta-aatgttccagGATTCCCAATCCTATGCCTTGAGTGGCAGAAGATGGAAAATCCTGATTTGCGAATGGCAATGGGAATGAAACCTGATCAGAAGGGCGTTCGTGTTAGAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|61117930|gb|DN558891.1|DN558891
EST:     CTGGAGAATACACAG                         GCTTCCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCGGATCTCC
genomic: CTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCC
EST: gi|19350981|gb|BM895513.1|BM895513
EST:     AGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAG                         GCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAACCCGGATCTCC
genomic: AGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCC
EST: gi|211497236|gb|FL425179.1|FL425179
EST:     AGAATACACAG                         GCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAACCCGGATCTCC
genomic: AGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCC
EST: gi|22491533|gb|BU051456.1|BU051456
EST:     AGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAG                         GCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAACCCGGATCTCC
genomic: AGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCC
EST: gi|89762755|gb|DY690219.1|DY690219
EST:     GTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAG                         GCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCC
genomic: GTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 122

TCTCTTAAACATGAAGATGCGGAGAACATAGGTTATGTGATACCGACTCCAGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 65

TCTCTTAAACATGAAGATGCGGAGAACATAGGTTATGTGATACCGACTCCAGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 89

TCTCTTAAACATGAAGATGCGGAGAACATAGGTTATGTGATACCGACTCCAGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 109

TCTCTTAAACATGAAGATGCGGAGAACATAGGTTATGTGATACCGACTCCAGTTATTACACATTTCATTGAGGACTACAAAAAATCTGGAGAATACACAGgtaatatgct ... attattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atctttctaatttccaatgacagtgttactatattctttttatgtattattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG
    ttctaat  putative branch site (score: 109)
                                              ttatttt  putative PPT
                       tgttactatattcttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ctccatgtaatgtttagtactatgatgttcatgcctacctcttagatctttctaatttccaatgacagtgttactatattctttttatgtattattttagGCTTTCCTATACTTGGAGTTGAATGGCAGAAAATGGAGAATCCAGATCTCCGCAAAGCAATGGGAATGAAACCAGATCAAAAGGGTGTTCGTGTTAGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGATCA
ctccatg
- - - - - - - - - - tatgatg
- - - - - - - - - - - - - -ttcatgc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgttact