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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gcatgtgatttccttatcatacttaaatgcataccttctctcattcactacattcatacatgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG

Basic information

species Zea mays
transcript GRMZM2G014805_T01
intron # 29
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: LOC_Os12g30540.1 (Oryza sativa), 3'ss of exon 30
----------------gcatg-tgatttccttatcatacttaaatgcataccttctctcattcactac-attcatac-atgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
                 |||| |      ||   |||    | |||  ||||| || |  || ||  |  ||||| | ||||||  |  ||| | ||| | |||||||||||||| ||||| |||||||||||||||||||||||||| || || || |||||| | ||||||||||| ||||||||||||
gcaagttataattttaacatgctaggaagctaggcatgtccatatgtgtacctccttttcttgaccgctgttcatgccatgatgtat--cttctgattctgcagAGGAGAGATGTTTATGGAGCTTGGGAACAATACCTTGGCTTGGAACATACTGACACAACACCAAAAAGGTCTTATACAGCCAATCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA20G11330.1 (Glycine max), 3'ss of exon 22
----gcatgtgatt-tccttatcatacttaaatgcataccttctctcattcac---tacattcat----acatgatgctttgctttttattatac---agAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
      |  | ||| |  || |  | ||||||   ||| |  || | |||||    |  |||  |    ||||  | |||   ||  |||  ||    |||| |||||||| |||||||||||||| ||||| ||||| |||||||| || || |   |  | ||||| || ||  | | ||| |||
gttgagacttcattgtaattttttttcttaaaaagatatccgctat-attcatgtttgaatttgtgattacattgtacttgttttggtatcctatgatagAGAAGAGATGTTTATGGTGCTTGGGAGCAATATCTTGGTTTGGAGCATACTGACAATGCTCCTAAAAGATCATATGCTGTCAACCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA02G43930.1 (Glycine max), 3'ss of exon 30
--------------------gcatgtgatttccttatcatacttaaatgcataccttctctcattcactacattcatacatgatgctttgctttttattat---acagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
                         |  |||  || | ||| || |||  |||  |    |||||   || ||   |  ||      |||| || |||  |   | ||||||||||||| |||||||||||||| ||||| ||||| |||||||| || ||||   |  | ||||| || ||  | |  || |||
gtacattcatttatacttggattccttttttttttgtgataattcaattgatatttagcatcattt-ttatatgtctgaatttatgcttgcattgtatcttgtgatagAGGAGAGATGTTTATGGTGCTTGGGAGCAATATCTTGGTTTGGAGCATACTGATAATGCTCCTAAAAGATCATATGCTGTTAACCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA13G16040.1 (Glycine max), 3'ss of exon 11
--------------gcatgtgatttccttatcatact--taaatgcataccttctctcattcactacattcatacatgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
              | |  |  ||| ||||  |   |    | || || | |  | | |||    |      | ||      || |  |  ||| ||  |||| |||||||| |||||||||||||| ||||| |||||||||||||| || || |   |  | ||||| || ||  | | ||| |||
gttgagacttcattgtaattttttttcttaaaaagttatccactgtattcatgtttttatttgtgattacattgcacttgttttggtatcctatgat--agAGAAGAGATGTTTATGGTGCTTGGGAGCAATATCTTGGCTTGGAGCATACTGACAATGCTCCTAAAAGATCATATGCTGTCAACCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA14G04890.1 (Glycine max), 3'ss of exon 30
-------------------------gcatgtgatttccttatcatacttaaatgcataccttctctcattcac-tacattcatacatgatgctt-tgctttttattat-acagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
                            | |  ||   || | ||| || |||  |||  |     ||||  | ||  |    |  | ||||||    | | | || | | ||||||||||||| |||||||||||||| || || |||||||||||||| || || |   |  | ||||| || ||  | |  || |||
gtacattcatttatacttggattccttttttttttggtttgtgataattcaattgatatttagcaccattttcatatgtctgaattt-atgcttgcattgtatcttgtgatagAGGAGAGATGTTTATGGTGCTTGGGAGCAGTATCTTGGCTTGGAGCATACTGACAATGCTCCTAAAAGATCATATGCTGTTAACCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: AT3G11910.1 (Arabidopsis thaliana), 3'ss of exon 30
----gcatgtgatttccttatcatacttaaatgcataccttctctcattcactacattcatacatgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
    |  ||| ||      ||| |||| |||   | |     |   ||| |   | ||  |   ||     |     |      ||||||| ||||||||||| ||||| ||||| || || ||||| ||||||||||  ||||   |  | |||||| ||||  | || ||||||
gtaaggctgttatccttagatc-tactcaaaaagaaa-----taggatttaactcttttgtctttggaatctaaaaatggc---tacagAGAAGAGATGTATACGGTGCGTGGGAGCAGTATCTTGGGTTGGAGCACATTGATAATGCTCCTAAAAGGGCTTATGCTGCAAATCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: Vv08s0032g00600.t01 (Vitis vinifera), 3'ss of exon 30
--------------------------------gcatgt-gatttcct-tatcatactta-aatgcatacct-tctctcattcactacattcatacat--gatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
                                | |||  |||  ||| |   | ||||   ||  || |   |      || | |  |||   |  |   ||| |||| ||| | || | ||||| |||||||| ||||||||||||||||| ||||||||  |||||||| | |||||  |  | |||||| ||||  |||| ||||||
gtgtcttaaatttaaaattatttttttcttgtgaatgccgatggcctgtgcaaaacttgtgatttatcctgatagggtttttattgaatttcgagtttaaatgttttggtttattttgttcagAGAAGAGATGTTTATGGTGCTTGGGAACAGTACCTTGGGCTGGAGCACTCTGATACTGCTCCTAAAAGGGCTTATGCAGCAAATCAG

upper sequence: GRMZM2G014805_T02 (Zea mays), 3'ss of exon 11
lower sequence: Vv13s0073g00610.t01 (Vitis vinifera), 3'ss of exon 30
gcatgtgatttccttatcatacttaaatgcatac-cttctctcattcactacattcatacatgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
|  | |  ||  ||     |   |   |  ||   |  ||    |  | | || |  |||   ||  |||  |||||  | | ||||| |||||||| |||||||||||||| || || ||||| ||||||||| |||| |   |  | |||||||| ||  |||| ||||||
gtctctctttctctctctctctctctctcaatgtgccacttgtttggattccacttttacccaat-ttttattttttgcttttcagAGAAGAGATGTCTATGGTGCTTGGGAGCAGTATCTTGGTTTGGAGCACTCCGACAATGCTCCCAAAAGGTCATATGCAGCTAATCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32944375|gb|CF049194.1|CF049194
EST:     GTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: GTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|22490260|gb|BU050183.1|BU050183
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|211029627|gb|FL157472.1|FL157472
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|18654871|gb|BM499676.1|BM499676
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|32833426|gb|CD973104.1|CD973104
EST:     ACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|18654872|gb|BM499677.1|BM499677
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGCGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|211029629|gb|FL157474.1|FL157474
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGATCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|87152609|gb|DY397398.1|DY397398
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|60400188|gb|DN232995.1|DN232995
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|78025615|gb|DV494002.1|DV494002
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|60400323|gb|DN233130.1|DN233130
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|211029628|gb|FL157473.1|FL157473
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|76019060|gb|DT946230.1|DT946230
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATAC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATAC
EST: gi|76019059|gb|DT946229.1|DT946229
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|211029631|gb|FL157476.1|FL157476
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|18963174|gb|BM660282.1|BM660282
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|211029630|gb|FL157475.1|FL157475
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|4827730|gb|AI668422.1|AI668422
EST:     ACCGGCCAGAGT-CCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|32934161|gb|CF038973.1|CF038973
EST:     CCGGGCAG-GTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: CCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|166670887|gb|EG297611.1|EG297611
EST:     ATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|32940670|gb|CF045489.1|CF045489
EST:     ACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|33094794|gb|CF054788.1|CF054788
EST:     GTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACCAATACCTTGGCTTGGAGCACAC
genomic: GTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAA-CAATACCTTGGCTTGGAGCACAC
EST: gi|18661897|gb|BM501640.1|BM501640
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAG                         AGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACC
EST: gi|94476779|gb|EB705737.1|EB705737
EST:     ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAA                         AGGAGAGATGTATATGGTGCTTGGGAACAATACC
genomic: ACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 277

TGGAAGCTTGCATTCATATCCATGAACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 288

TGGAAGCTTGCATTCATATCCATGAACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 387

TGGAAGCTTGCATTCATATCCATGAACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 215

TGGAAGCTTGCATTCATATCCATGAACCGGCCAGAGTACCTCCAGGACACTGATGTTGTATCAGCACGTTTTCAGgcatgtgatt ... tattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ataccttctctcattcactacattcatacatgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG
                                   ctttgctttttatt  CT-rich tract
                                    tttgctttttattata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gcatgtgatttccttatcatacttaaatgcataccttctctcattcactacattcatacatgatgctttgctttttattatacagAGGAGAGATGTATATGGTGCTTGGGAACAATACCTTGGCTTGGAGCACACCGATACAACATCGAAAAGGTCTTACACAGCCAATCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCA