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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tttgctttattcacttagaactaaagatttattatttagttatgctgtctgctagaattttctttcttcgctaagaactaaagattatgtttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA

Basic information

species Zea mays
transcript GRMZM2G162531_T02
intron # 26
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211336847|gb|FL296078.1|FL296078
EST:     ATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAG                         GCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTC
genomic: ATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAGgtttgtttac ... ttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTC
EST: gi|32833103|gb|CD972781.1|CD972781
EST:     ATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAG                         GCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTC
genomic: ATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAGgtttgtttac ... ttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 590

GGGTGAATTAGTTGGCGCTCACCTTATTGCACATGGTGGAAGTTTGCTAAATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAGgtttgtttac ... ttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 290

GGGTGAATTAGTTGGCGCTCACCTTATTGCACATGGTGGAAGTTTGCTAAATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAGgtttgtttac ... ttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 377

GGGTGAATTAGTTGGCGCTCACCTTATTGCACATGGTGGAAGTTTGCTAAATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAGgtttgtttac ... ttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 172

GGGTGAATTAGTTGGCGCTCACCTTATTGCACATGGTGGAAGTTTGCTAAATTTGGCCAAGCAGCCTGGTAGCACAATTCAGATACTTGGTGCAGAGAAGgtttgtttac ... ttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtctgctagaattttctttcttcgctaagaactaaagattatgtttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA
                              aactaaa  putative branch site (score: 172)
                                            tttctcacc  putative PPT
                                 taaagattatgttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tttgctttattcacttagaactaaagatttattatttagttatgctgtctgctagaattttctttcttcgctaagaactaaagattatgtttctcaccagGCTTTATTCAGAGCTCTAAAGACAAAGCATTCTACACCTAAGTATGGCCTCATCTACCATGCATCCTTAATTGGCAAGGCTTCTCAAAAGCACAAGGGAA

tttgctt
- - - - - - - - - aactaaa
- - - - - - - - - - - - - - - - - - - - - tgctgtc