ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttcctttcctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT

Basic information

species	Zea mays
transcript	GRMZM5G803949_T03
intron #	2
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G803949_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os02g02840.1 (Oryza sativa), 3'ss of exon 2

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttcctttcctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT
                                      | ||    ||    | || ||   | |   ||     |||||||    ||    | |  || || ||   || ||   | |   | |||||| ||||||||||||||||||||||||||||| |||||||||||||||||||| |||||||| ||||| || ||||| || |||||||||||||||||
-------------------------------------gtaatctggttgcgctgctatctaattctgcatgcttgttttcttctacca----agttattaagtttt--tccttcggaataccacagGTCAGGAGGATTACAACAGACTGAGACCACTGAGTTATCGTGGAGCTGATGTTTTCCTTCTGGCCTTCTCGCTAATCAGCAAGGCCAGCTATGAGAATGT

upper sequence: GRMZM5G803949_T02 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA04G02530.3 (Glycine max), 3'ss of exon 2

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttcctttcctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT
                   |  | || |     | ||    |   |  ||||   | || ||| |   ||||  ||| | | ||         |||||| | | |     ||        ||| |||||| ||||||||||| ||| | |||||  |||||||||| ||||||||||| ||  | || || || || || || || || || |||||||| ||| |
------------------gtaacatggcaaaacaattcaattatatagttttcagtatcattttctgcatattgtttgtttctg---------atggtttaacatattgtttgat-----taacagGTCAGGAGGATTACAATAGATTAAGACCCTTGAGCTATCGAGGAGCTGATGTCTTCATACTTGCCTTTTCTCTCATAAGCAAGGCTAGCTATGAAAATAT

upper sequence: GRMZM5G803949_T02 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA11G11510.1 (Glycine max), 3'ss of exon 2

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttcctttcctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT
                                   | |  ||  |||||  |   | || ||    |    | ||| |  |||||       | || ||   | |   | |   |   ||  | ||| ||||| |||||||| ||| |||||||  | |||||||||||||| ||||| || || || |||||||| || || || |  ||||||||||| |||||
----------------------------------gtaagtatagtttttcctggttatctttcatcc----ttcttgtgttttgt------gagtgttttgtcattgttttcaacttatggcacagGCCAAGAAGATTACAATAGATTGAGACCCTTAAGCTATCGTGGAGCAGATGTATTCCTGCTTGCTTTCTCTCTCATAAGCAGGGCCAGCTATGAAAATGT

upper sequence: GRMZM5G803949_T02 (Zea mays), 3'ss of exon 2
lower sequence: Vv18s0001g14670.t01 (Vitis vinifera), 3'ss of exon 2

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttcctttcctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT
                        |   ||  |    ||  | ||   ||| |   ||| |||   |  | ||||  ||  |||||  |   ||| |   |  ||| || ||         || ||| || ||||||||||| ||| | |||||  |||||||||| || ||||| |||||| |||| || ||||| || || || || ||||||||||| |||||
-----------------------gtaatcttatgaacatccttctggatttcttgattcttttat-cagtttcctcatttcttgttttt---aattgactatattcgattttg--------aacagGCCAGGAGGATTACAATAGATTAAGACCCTTGAGCTATCGAGGGGCTGACGTTTTTATTCTTGCATTCTCTCTCATTAGCAAGGCCAGCTATGAAAATGT

upper sequence: GRMZM5G803949_T02 (Zea mays), 3'ss of exon 2
lower sequence: Vv04s0023g03860.t01 (Vitis vinifera), 3'ss of exon 3

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttcctttcctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT
                        |  | |   | | |   || ||  |||||  ||| |  |     | |  | ||||  || ||       |||| |  |  |   |  |      || || ||| || ||||||||||||||| |||||||  |||| ||||| || || ||||||||  |  |||| ||||| || ||||| || ||||||||||| |||||
-----------------------gtagcttcacagtcact-tgtttccttttttgttttcaattactaacaaacattttgattatttt-----atggctctgaatttgttctc---tttggaacagGGCAGGAGGATTACAACAGATTGAGACCTTTGAGTTATCGCGGGGCAGATGTTTTCATATTGGCATTCTCTCTCATCAGCAAGGCCAGCTATGAAAATGT

upper sequence: GRMZM5G803949_T02 (Zea mays), 3'ss of exon 2
lower sequence: PP1S126_126V6.2 (Physcomitrella patens), 3'ss of exon 3

gtgactagccgtgctgttctgtcctgtcctgctacttatgatgctttttttttccttt-cctttgcgcgatatcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT
                ||  ||| ||       ||  |  |   |   ||     |   ||  |  |  || || | || | ||  |||   |||   || | || ||  || ||||         |||||||| ||||||||||||||  |||| ||||| ||  | || ||||||||||||||||| || || || || ||||| || || || ||||| ||| |
---------------ttttggtcttgctgattcacacacaaccttccattggaagtcgaccccttagttatttcttattccatttgtcactgaaaacattta-ctacc--ctatgca---------gGTCAAGAAGATTACAACAGACTTCGACCTCTGAGTTACAGAGGCGCTGATGTTTTTCTTCTAGCATTTTCTCTTATCAGCAAGGCTAGTTATGAAAATAT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211356952|gb|FL222516.1|FL222516

EST:     CGTTGTGGTTGATGGTAACACTGTCAACCTCGGCCTCTGGGACACTGCAG                         GTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATG
genomic: CGTTGTGGTTGATGGTAACACTGTCAACCTCGGCCTCTGGGACACTGCAGgtgactagcc ... actgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 198

GACTATGTTCCGACGGTGTTTGACAACTTCAGTGCTAACGTTGTGGTTGATGGTAACACTGTCAACCTCGGCCTCTGGGACACTGCAGgtgactagcc ... actgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 68

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 148

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 507

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tcctttttctttgttcatcaaaatggttgagcttccatctttgcaactgtaatagGTCAAGAGGATTACAACAGACTGAGACCACTGAGCTATCGTGGAGCTGATGTTTTTCTTCTGGCTTTCTCACTGATCAGTAAAGCCAGCTATGAGAATGT