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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaatcaaataatgcatgccactgtatcaggacacccgttggaacttgtgaagctctctgactgtagaatcatcttttttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG

Basic information

species Zea mays
transcript GRMZM2G339336_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G339336_T01 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os05g25310.2 (Oryza sativa), 3'ss of exon 2
gtaatca-aataatgcatgccactgtatcaggacacccgttggaacttgtgaagctctctgactgtagaatcatcttttttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG
|| || | |||||    ||   || | |    |||   ||  |||| | |||| || | ||||  ||  ||  | || ||| | | |||||||||| ||||||||| |||| |||||||| || |||||||||||||| ||||||||| ||||||||||||| ||||||
gtgattataataaatagtgtggctttctgtatacatttgtgagaacctatgaacctgtatgacaata--attgttttattt-tgtcagGGATGCTTTCGACAAAATCTAACAGTTGTAACTATCTATGCATCTCTTGGCGAGGATGCACTGGTGCATTCACTGAATGAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149094834|gb|EE180405.2|EE180405
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGGTA-CAATATATGCATCT
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTG-TAACAATATATGCATCT
EST: gi|211474586|gb|FL313884.1|FL313884
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
EST: gi|211377763|gb|FL319190.1|FL319190
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
EST: gi|211200655|gb|FL341862.1|FL341862
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
EST: gi|33771105|gb|CF272766.1|CF272766
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
EST: gi|60353341|gb|DN220314.1|DN220314
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGT
EST: gi|149111542|gb|EE190675.2|EE190675
EST:     ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAG                         GGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATG
genomic: ATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 394

ATGTAATTTTTCTTCTGGACTTATCAAGATGGGCCACGACCTAGATAGTCATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 701

ATGTAATTTTTCTTCTGGACTTATCAAGATGGGCCACGACCTAGATAGTCATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 556

ATGTAATTTTTCTTCTGGACTTATCAAGATGGGCCACGACCTAGATAGTCATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 933

ATGTAATTTTTCTTCTGGACTTATCAAGATGGGCCACGACCTAGATAGTCATGCTGCTATCTTCTCTGATACAAGAGCTGAGTGGATCATTGCTGCTCAGgtaatcaaat ... tttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cacccgttggaacttgtgaagctctctgactgtagaatcatcttttttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG
                       ctctgac  putative branch site (score: 933)
                                     tcatcttttttctttt  putative PPT
                                       atcttttttctttta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaatcaaataatgcatgccactgtatcaggacacccgttggaacttgtgaagctctctgactgtagaatcatcttttttcttttagGGATGCTTCCGACAAAATTTAACGGTTGTAACAATATATGCATCTCTTGGTGAGGATGCATTGGTGCATTCACTAAATGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGGATG