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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tacaattatgtctgccatataattccttggtggttttgatttccattcggaaagaaaaaagaatcagaaagtgacatgaaagaaactcttttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT

Basic information

species Zea mays
transcript GRMZM2G179429_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G179429_T03 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os07g01320.1 (Oryza sativa), 3'ss of exon 3
tacaattatgtctgccatataattccttggtggt--tttgatttccattcg--gaaagaaaaaagaatcagaaagtgacatgaaagaaactcttttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT
 | ||  |||    ||| |   ||| |  |   |  |||| | |   |||    ||||    |||  |   | | | |   | |  ||||| | | ||||| ||||||||||| | || ||||||||  |||||| ||||||||| || || |  ||||||||||||||||| || || |||||||||||||||||||| |   
-ataacaatgg--accaaaactttcatatgcattcctttgctgtttgttcactaaaagttccaagtttttcacattcatgcgcac-aaactgtctacttgccagGCCGAAGAAGCAGCCCGGGATTCGAGGAGGACTAAACAAGATCGCTATGCAGAAATGAGGAGGAAGAAAGACGAAGAGCGTGAGGCACAAGAGAGACTTA

upper sequence: GRMZM2G179429_T03 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA13G36410.1 (Glycine max), 3'ss of exon 3
----tacaattatgtctgccatataattccttggtggttttgatttccattcggaaagaaaaaagaatcagaaagtgacatgaaagaaactcttttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT
    | |    || ||||   | ||||   ||| |  ||  |   |   |       ||  ||||  | | | |||   ||    |   || | |  ||| ||||| || |||  |||||| || ||| ||      |||||||| || ||    |||||| |||||  |||||||||||||||||||||||| ||| |  | |
gtatttcttgaatttctgtgct-taatgttttgctttttggggggtttctctatgttgattaaagt-ttaaatagttgtattttgg--tctatctctttggaagGCTGAGGAAGTTGCACGAGAGTCAAGGCTTGCAAAACAAGATCGTTATTCAGAAATGCGGAGGTTGAAGGATGAGGAGCGTGAGGCACAGGAGCGTAAGT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|31364620|gb|CD448975.1|CD448975
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAT
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|71757661|gb|DR955598.1|DR955598
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211266946|gb|FK962386.1|FK962386
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCAC
EST: gi|50329493|gb|CO524619.1|CO524619
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211271834|gb|FK962420.1|FK962420
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGA
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGA
EST: gi|89248029|gb|DY619815.1|DY619815
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|31909721|gb|CD651061.1|CD651061
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|91057069|gb|EB167487.1|EB167487
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|91048409|gb|EB158827.1|EB158827
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|76014913|gb|DT942083.1|DT942083
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211269390|gb|FK962402.1|FK962402
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAAC-TGCACGGGAT-CATGGAGGAGTAA
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAA
EST: gi|211269397|gb|FK962409.1|FK962409
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|93283894|gb|EB676158.1|EB676158
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|18383735|gb|BM416935.1|BM416935
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAT
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211266949|gb|FK962389.1|FK962389
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAAC
EST: gi|78112542|gb|DV530936.1|DV530936
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|18384245|gb|BM417444.1|BM417444
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAT
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|148958245|gb|EC904458.2|EC904458
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|71307153|gb|DR789747.1|DR789747
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|71773571|gb|DR971469.1|DR971469
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211269392|gb|FK962404.1|FK962404
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGT
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGT
EST: gi|211266944|gb|FK962384.1|FK962384
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAAACTGCACGGGATTCATGGAGGAGTAAA
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAA
EST: gi|60357983|gb|DN224956.1|DN224956
EST:     CAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: CAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|87153892|gb|DY398681.1|DY398681
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAT
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|148956262|gb|EC902060.2|EC902060
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211266948|gb|FK962388.1|FK962388
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGG
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGG
EST: gi|148959747|gb|EC905644.2|EC905644
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|148932548|gb|EC876363.2|EC876363
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAT
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGAC
EST: gi|211271831|gb|FK962417.1|FK962417
EST:     GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAG                         GCCGAAGAACCTGCACGGGA
genomic: GATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 153

CGACGCGGAGAGTGACGATGAGGAGGTCCCCGAAGGTCGCAACGTACCCAGATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 100

CGACGCGGAGAGTGACGATGAGGAGGTCCCCGAAGGTCGCAACGTACCCAGATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 87

CGACGCGGAGAGTGACGATGAGGAGGTCCCCGAAGGTCGCAACGTACCCAGATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 111

CGACGCGGAGAGTGACGATGAGGAGGTCCCCGAAGGTCGCAACGTACCCAGATCCTCCAAAAAGGAGAAGAAAAGGCAAGAGCGAGAGATGCAGCGTCAGgttggttact ... ttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttcggaaagaaaaaagaatcagaaagtgacatgaaagaaactcttttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT
                                        ctcttttcttgc  CT-rich tract
     aaagaaaaaagaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tacaattatgtctgccatataattccttggtggttttgatttccattcggaaagaaaaaagaatcagaaagtgacatgaaagaaactcttttcttgcaagGCCGAAGAACCTGCACGGGATTCATGGAGGAGTAAACAAGACCGATACGACGAAATGAGGAGGAAGAAGGATGAGGAGCGTGAGGCACAAGAGAGGCAAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGAGG
- - - - tgtctgc
- - - - - - - - - - - - - -tggtggt
- - - - - - - - - - - - - - - - - - - - - - - - - aaagaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - -gaaaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgacatg