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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagttatttgcatattttgtccattattttgttgtctacttgtctcgtcttatttttgtgttgaaaacattgtatgtccaattcatgcgtgcttcattattaaactgatcattttgaactgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG

Basic information

species Zea mays
transcript GRMZM2G176506_T04
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G176506_T04 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g09120.2 (Oryza sativa), 3'ss of exon 2
gtgagttatttgcatattttgtccattattttgttgtctacttgtctcgtcttat-ttttgtgttgaaaacattgtatgtccaattcatgcgtgcttcattattaaactga----tcattttgaactgat-cacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG
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gtgagttatttgcataatctgt---ttgtattgttgt-----tgttttgacgtatatctcgtgttcaaaataa-atatatgcatttcattccctctttattaattgagagcagttttattttgaaatgattcacagGACTGACCCCCATTTGCCTGACATGCCGATCATCTATGCTAGTGATGCTTTTCTATCACTAACAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78077483|gb|DV505917.1|DV505917
EST:     AGTTCGTCGTTAAAACTCTCTCTCGGTAGAATCAAACAGAGCTTTGTATT                         GACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTT
genomic: AGTTCGTCGTTAAAACTCTCTCTCGGTAGAATCAAACAGAGCTTTGTATTgtgagttatt ... ctgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTT
EST: gi|166680836|gb|EG140230.1|EG140230
EST:     CAAACAGAGCTTTGTATT                         GACTGACTCCCGCTTGCCTGACATGCCAATTATTTATGCTAGTGATGCTTT
genomic: CAAACAGAGCTTTGTATTgtgagttatt ... ctgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 42

GTCTAGTGGTCAGCAGGAAGAGATGTGACTCGATTGGCATCCCAGCACTCAGTTCGTCGTTAAAACTCTCTCTCGGTAGAATCAAACAGAGCTTTGTATTgtgagttatt ... ctgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG


Block sizes: 46 (upstream exon), 38 (downstream exon)
Score: 50

GTCTAGTGGTCAGCAGGAAGAGATGTGACTCGATTGGCATCCCAGCACTCAGTTCGTCGTTAAAACTCTCTCTCGGTAGAATCAAACAGAGCTTTGTATTgtgagttatt ... ctgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG


Block sizes: 49 (upstream exon), 42 (downstream exon)
Score: 34

GTCTAGTGGTCAGCAGGAAGAGATGTGACTCGATTGGCATCCCAGCACTCAGTTCGTCGTTAAAACTCTCTCTCGGTAGAATCAAACAGAGCTTTGTATTgtgagttatt ... ctgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG


Block sizes: 49 (upstream exon), 43 (downstream exon)
Score: 49

GTCTAGTGGTCAGCAGGAAGAGATGTGACTCGATTGGCATCCCAGCACTCAGTTCGTCGTTAAAACTCTCTCTCGGTAGAATCAAACAGAGCTTTGTATTgtgagttatt ... ctgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atgtccaattcatgcgtgcttcattattaaactgatcattttgaactgatcacagGACTGACTCCCGCTTGCCTAACATGCCAATTATTTATGCTAGTGATGCTTTTACATCATTAACAG
                                           aactgat  putative branch site (score: 49)
                   ttcattattaaact  TA-rich tract