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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgccacgagttcccctcccctggatccgtttccctatctcaatctcatcctccggtcttgtgctcggaggagctcagtagccaataggggtagaccgcatgtagctcgaaattgtcgagcgtcgcgctgttttgttctcgtgaaatgattttgtgcttctctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG

Basic information

species Zea mays
transcript GRMZM2G165535_T05
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G165535_T05 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os04g58580.1 (Oryza sativa), 3'ss of exon 1
gtgccacgagttcccctcccctggatccgtttccctatctcaatctcatcctccggtcttgtgctcggaggagctcagtagccaataggggtagaccgcatgtagctcgaaattgtcgagcgtcgcgctgttttgttctcgtgaaatgattttgtgcttctctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
       |  | ||| | | | ||||||| |||| |      ||||||||      ||||   | ||   ||    |||  ||  | |  | || | ||||      ||   |||| ||  |   |  |||| |  |||           ||| |  ||    ||   ||| ||||||||| ||||||||  |||||||||||||||||||| ||
-------gtttccccattctccggatccgcttccgtcatctcatctcatc------tctt---cccgagcga---tagtttcc--tcgctgcag-ctgcat------cgtcgttgttgaatgaatc-ttgttcttctctt---------cttttttttttgggggtatagGTGGTGACGCCGGAGATGCTCCAGTTCATGAAGCAGCTGCGCGAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32919710|gb|CF024522.1|CF024522
EST:     GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAG                         GGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
genomic: GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
EST: gi|71771666|gb|DR969603.1|DR969603
EST:     GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAG                         GGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
genomic: GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
EST: gi|148950412|gb|EC895979.2|EC895979
EST:     GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAG                         GGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
genomic: GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
EST: gi|148943663|gb|EC888727.2|EC888727
EST:     GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAG                         GGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
genomic: GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
EST: gi|76280415|gb|DV019983.1|DV019983
EST:     GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAG                         GGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
genomic: GCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 98

GGACTTGAGGAGCGGGTGGGTTGCGATGGCCGCTGCGGGGAGGAATGCGGGCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 172

GGACTTGAGGAGCGGGTGGGTTGCGATGGCCGCTGCGGGGAGGAATGCGGGCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 215

GGACTTGAGGAGCGGGTGGGTTGCGATGGCCGCTGCGGGGAGGAATGCGGGCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 157

GGACTTGAGGAGCGGGTGGGTTGCGATGGCCGCTGCGGGGAGGAATGCGGGCGTCCTCGCGCTCTTCGACGTCGACGGCACCCTCACAGCGCCGCGCAAGgtgccacgag ... tctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcgagcgtcgcgctgttttgttctcgtgaaatgattttgtgcttctctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG
                                  ttttgtgcttctct  CT-rich tract
                            aaatgatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgccacgagttcccctcccctggatccgtttccctatctcaatctcatcctccggtcttgtgctcggaggagctcagtagccaataggggtagaccgcatgtagctcgaaattgtcgagcgtcgcgctgttttgttctcgtgaaatgattttgtgcttctctggggcagGGGGTGACGCCAGAGATGCTAGAGTTCATGAAGCAGCTGCGCCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC