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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...attcaacaaaatatatgtttgagttgttttttttaaatgactagttgtttcaatgcttcagactttagagactgaacttctcatgcttcaaatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC

Basic information

species Zea mays
transcript GRMZM2G143525_T03
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G143525_T03 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os06g24594.6 (Oryza sativa), 3'ss of exon 3
at-tcaacaaaatatatgtttgagttgttttttttaaatgactagttgtttcaatgcttcagactttagagactgaacttctcatgcttc---aaatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC
|| ||  |  |||  |||  ||     ||    ||||  |  |  |  |||  || ||  | | |      | || | | ||| ||  |    |||| | ||||||| || |||||| ||||||||||||| ||||| ||||||| |||||||||||| || |||||| | || |||||||| ||||| || ||||||||||||
atatctccttaattcatggctgga--attcgagttaacag--tgatgctttagattctatatattactatcagtgtaatcctcgtgtgttcataaatattgcagTGGGAATTGGGATCTGAAGACAAAGGTACCTGAACAGTGCAGGGTTTCAAAAATTAAGTTACCTTCTTACTTTATGGAGTGGATCAATTTGAAGGATATC

upper sequence: GRMZM2G143525_T03 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os06g24594.5 (Oryza sativa), 3'ss of exon 5
------------------------------------------------attcaacaaaatatatgtttgagttgttttt----tttaaatgactagt--tgtttca-atgcttcagactttagagactgaacttctcatgcttc---aaatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC
                                                ||  |  | | ||| |  || | |  |   |    ||  | | |  |||  || || | || ||  | | |      | || | | ||| ||  |    |||| | ||||||| || |||||| ||||||||||||| ||||| ||||||| |||                                                       
gtatgtcatactttgtttcacaaattttcttggtgggttttgtgttgcatctagtaca-tatctccttaattcatggctggaattcgagttaacagtgatgctttagattctatatattactatcagtgtaatcctcgtgtgttcataaatattgcagTGGGAATTGGGATCTGAAGACAAAGGTACCTGAACAGTGCAGGGT-------------------------------------------------------

upper sequence: GRMZM2G143525_T03 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA06G35090.1 (Glycine max), 3'ss of exon 4
------------------------------------------attcaacaaaatatatgtttgagttgttttttttaaatgactag-ttgtttcaatgcttcagactttagagactgaacttctcatgcttcaaatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC
                                          |||     |  | ||    ||  || ||||  ||   ||  ||  |||    |      |||| ||| |    |  || ||  |  |||    | | |||||||||| ||||||||||| |||||||||||| |||| ||  | || ||||| ||| |  | ||| | ||||||||||| ||||||||  | || ||| ||
gtaaatatcattgcttatggtttaccatttatacctcttttcattttcttatttgtagtggtggattattttccttccctgtatatcttgaaatagaagaccagaattt-gttcttttacatcaaaatcttttgctattgcagTGGAAATTGGGATTTGAAAACAAAGGTCCCTCAGCAATGTGAAGTGTCAAAGATAGAGCTTCCTCCATATTTTATGGAGTGGATTAATCTCAAAGATGTC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211067166|gb|FL447230.1|FL447230
EST:     GAGCATTTGTTACTTG                         TGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
genomic: GAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
EST: gi|71758607|gb|DR956544.1|DR956544
EST:     TGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTG                         TGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
genomic: TGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
EST: gi|211396340|gb|FL436997.1|FL436997
EST:     GATCACTCAACCGTGGAGCATTTGTTACTTG                         TGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
genomic: GATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
EST: gi|4647496|gb|AI622571.1|AI622571
EST:     TGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTG                         TGGAAANCTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAA
genomic: TGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAA-CTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAA
EST: gi|16380290|gb|BI993413.1|BI993413
EST:     TGGAAAAAGGAGCAAGGTGGATCACTCAGCCGTGGAGCATTTGTTACTTG                         TGGAATCTGGGATTTGAAGTCAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA
genomic: TGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 73

TCAAGCAAGTGCTGCAAGAGTTTGAGGACTGGCTTGGAAATCACAACCTGTGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 81

TCAAGCAAGTGCTGCAAGAGTTTGAGGACTGGCTTGGAAATCACAACCTGTGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 45

TCAAGCAAGTGCTGCAAGAGTTTGAGGACTGGCTTGGAAATCACAACCTGTGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 49

TCAAGCAAGTGCTGCAAGAGTTTGAGGACTGGCTTGGAAATCACAACCTGTGGAAAAAGGAGCAAGGTGGATCACTCAACCGTGGAGCATTTGTTACTTGgtaacctaat ... aatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtttcaatgcttcagactttagagactgaacttctcatgcttcaaatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC
                                         ttcaaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
attcaacaaaatatatgtttgagttgttttttttaaatgactagttgtttcaatgcttcagactttagagactgaacttctcatgcttcaaatgtggcagTGGAAACTGGGATTTGAAGACAAAGGTCCCTGAGCAGTGCAAGGTTTCAAAAATAAATTTACCTACCTATTTTATGGAATGGATTAACTTGAAGGATATC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGAAG