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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agtgttccttttgcatgtagcatgcattgcacttttctgctgtgtgggaccttcatatctgcatatttatagctttggatgctgactgttgtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG

Basic information

species Zea mays
transcript GRMZM2G143246_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G143246_T01 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g10050.1 (Oryza sativa), 3'ss of exon 2
agtgttccttttgcatgtagcat-gcattgcacttttctgctgtgtgggaccttcatatctgcatatttatagctttggatgct-gactgttgtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG
|   |      | |||     ||    |||| ||||| |  | |  |  | |||    |||  || ||  |       ||| ||  |||    | ||  |||||||||| |||||||| || ||||||||||| ||||| |||||||| |||||||| || ||||||||||| |||||| |||| || || || ||||||||
accttctgaactacattctttattattttgctcttttttaatcttggacatcttacattctatat-ttcctgatccaagatacttaacttccattgtg-cagAATATGTTTTGCAAGAAATTACAAAAGCTGGGTTTGTAGAACCTACTCCTATCCAGTCTCAAGGTTGGCCAATGGCACTGAGGGGACGTGATCTTATTGG

upper sequence: GRMZM2G143246_T01 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA08G20670.1 (Glycine max), 3'ss of exon 2
agtgttccttttgcatg--tagcatgcattgcacttttctgctgtgtgggaccttcatatctgcatatttatagctttggatgctgactgttg--tggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG
  || |  |  |  |||  || | ||||       ||       | |||||    ||| | |    |   |  |   ||  |  | |   |||  |   | ||||||||||  ||||||| || ||||||||||| |||   |||||||| || || ||||| ||||||||||| ||||| ||||  || || || || || ||
tatgattatactatatgattatcttgcaaaatttattatcagattatgggatga-catgttt---caaacagcgtactgatttttcagccttggtttaatccagAATATGTACTGCAAGAAATTACAAAAGCTGGCTTTACTGAACCTACACCCATTCAATCTCAAGGTTGGCCCATGGCTTTGAAGGGGCGTGATCTGATAGG

upper sequence: GRMZM2G143246_T01 (Zea mays), 3'ss of exon 2
lower sequence: Vv09s0002g02100.t01 (Vitis vinifera), 3'ss of exon 2
agtgttccttttgcatgtagcatgcattgcacttttctgctgtg-----tgggaccttcatatctgcatatttatagctttggatgctgactgttgtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG
       |  |  |  | | | |   |||     || |||| ||     |||    || | |  || |  ||||    |||  |||   ||  |||||  | ||||||||||  ||||||| || ||||||||||| ||||  |||||||| || |||||| | ||||| ||||| ||||| ||||  ||||| ||  | |||||
---aaatcagtgactggcaacctttcttgagaagttatgctttggatgatggatattttaaaattgaagttttaattatttaaatgt--acctttgtgactccagAATATGTCATGCAAGAAATTACAAAAGCTGGCTTTGCTGAACCTACACCCATCCAAGCTCAAGGATGGCCAATGGCTTTGAAGGGCCGTGATGTCATTGG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32837635|gb|CD977313.1|CD977313
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|71774216|gb|DR972094.1|DR972094
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|148990097|gb|EE031861.2|EE031861
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAATTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|78081135|gb|DV509547.1|DV509547
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|211107541|gb|FL061182.1|FL061182
EST:     CGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CGCGACGTGCCCAAGCCGGTGCGTGAGTTCCG-TGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|149099803|gb|EE185855.2|EE185855
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|76287087|gb|DV026655.1|DV026655
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|94477391|gb|EB706349.1|EB706349
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|93017517|gb|EB643037.1|EB643037
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|91053059|gb|EB163477.1|EB163477
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|32837605|gb|CD977283.1|CD977283
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATTTTTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|32840396|gb|CD980077.1|CD980077
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|211107539|gb|FL061180.1|FL061180
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|78074504|gb|DV502938.1|DV502938
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|211108455|gb|FL061195.1|FL061195
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|78077833|gb|DV506262.1|DV506262
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|31359983|gb|CD444340.1|CD444340
EST:     GCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|78120999|gb|DV539383.1|DV539383
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|211108449|gb|FL061189.1|FL061189
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGGCTGGTTTTGTGGGACCTACCCCT
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAG-CTGGTTTTGTGGAACCTACCCCT
EST: gi|32838958|gb|CD978639.1|CD978639
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|211108453|gb|FL061193.1|FL061193
EST:     CCGCGACGTGCCCAAGCCGGTGCTTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|148938225|gb|EC882515.2|EC882515
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|149105515|gb|EE291040.2|EE291040
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|148952300|gb|EC898110.2|EC898110
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|149005479|gb|EE040035.2|EE040035
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|148931912|gb|EC875118.2|EC875118
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|78083647|gb|DV512040.1|DV512040
EST:     CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
EST: gi|88751705|gb|DY535846.1|DY535846
EST:     CCGCGACGTGCCCAAGCCGGTGCGAGAGTTCCGTGATGTGGGCTTCCCAG                         AATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA
genomic: CCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 127

GAGGAAGAGGTGGAGGCGTACCGCCGCCGCCGGGAGATCACCGTCGACGGCCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 43

GAGGAAGAGGTGGAGGCGTACCGCCGCCGCCGGGAGATCACCGTCGACGGCCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG


Block sizes: 40 (upstream exon), 49 (downstream exon)
Score: 70

GAGGAAGAGGTGGAGGCGTACCGCCGCCGCCGGGAGATCACCGTCGACGGCCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 88

GAGGAAGAGGTGGAGGCGTACCGCCGCCGCCGGGAGATCACCGTCGACGGCCGCGACGTGCCCAAGCCGGTGCGTGAGTTCCGTGATGTGGGCTTCCCAGgtaatgattt ... gtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gggaccttcatatctgcatatttatagctttggatgctgactgttgtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG
                                  tgctgac  putative branch site (score: 88)
                 atatttatagcttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agtgttccttttgcatgtagcatgcattgcacttttctgctgtgtgggaccttcatatctgcatatttatagctttggatgctgactgttgtggtttcagAATATGTGTTGCAAGAGATCACAAAAGCTGGTTTTGTGGAACCTACCCCTATCCAATCGCAAGGTTGGCCGATGGCATTGAGAGGCCGCGACCTTATTGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGAGG
- tgttcct
- - - - - -tgcatgt
- - - - - - - - - agcatgc
- - - - - - - - - - - - - -tgcactt
- - - - - - - - - - - - - - - - - -tctgctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctgcata
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gatgctg