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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tgtagatcttttggtttctttatacagatgtacatatacccttttatcttagaatgacagaattctcattatacatttgtacatatgttttatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT

Basic information

species Zea mays
transcript GRMZM2G134613_T03
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G134613_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os08g09240.2 (Oryza sativa), 3'ss of exon 1
tgtagatcttttggtttctttatacagatgtacata--tacccttttatct-tagaatgacagaat-tctcattatacatttgtacatatgttttatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT
| |  ||  || |        || |  ||  ||| |  |||    |||| | | | || |  |||  ||||| | |   ||| | |  |||| || | |||||||||||||||| |||||||| ||||||||||||||||| || || || ||||| |||
tatcaattcttagcgcataacat-cttatcaacagaattacttggttatgtgtggtataattgaagatctcactctg--tttatgc-catgtgttgtgtcgcagAAAGGAGGCAGGCTGAGGCCAACCGCATCAGGGAGAAGTATCCCGATAGAATTCCT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|116828492|gb|EC857917.1|EC857917
EST:     GAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCGCCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAGTACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|16921805|gb|BM075308.1|BM075308
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|16925527|gb|BM078595.1|BM078595
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGATAAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|67025573|gb|CO454322.1|CO454322
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|84980814|gb|DW531163.1|DW531163
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|5761920|gb|AI966893.1|AI966893
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|116815633|gb|EC858313.1|EC858313
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|211145629|gb|FL256802.1|FL256802
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|37374696|gb|CF623730.1|CF623730
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
EST: gi|67040113|gb|CO466368.1|CO466368
EST:     AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCG                         AAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA
genomic: AAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 476

GTCGCTTCTCTCTGTTCCGCGTGCGATCGAGTAGATCAAAAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 2215

GTCGCTTCTCTCTGTTCCGCGTGCGATCGAGTAGATCAAAAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1761

GTCGCTTCTCTCTGTTCCGCGTGCGATCGAGTAGATCAAAAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 1753

GTCGCTTCTCTCTGTTCCGCGTGCGATCGAGTAGATCAAAAGGAGGAGGAGGATGGCGAGGAGTTCGTTCAAGTTGGAGCACCCCTTCGgttcgtctct ... tatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atcttagaatgacagaattctcattatacatttgtacatatgttttatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT
                      attatacatttgtaca  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tgtagatcttttggtttctttatacagatgtacatatacccttttatcttagaatgacagaattctcattatacatttgtacatatgttttatatcgcagAAAGGAGGCAAGCTGAGGCTAACCGCATCAGGGAGAAATACCCTGACAGAATCCCT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAA