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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG

Basic information

species Zea mays
transcript GRMZM2G114793_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os02g02410.1 (Oryza sativa), 3'ss of exon 2
gtaacacctctct--cctacagttacttctgttgaatttcgtctcgttgtgggttatttctggc-gtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
||||   | || |  ||||   |  ||  | ||        ||  ||||| | | | | ||| | |   | || |   ||||     |||  |  ||||| |  ||||||||||| |||||||||||||||||||| |||||||||||| | |||||||| || |||||||||||||||||||||||||||||||| 
gtaatggcactttaccctatgctcgctcttatt--------tcctgttgttgatcactgctgcctgatctgacctgtataat----tattcttgcagGTTTGAAGACAAGGAAGTTCAGAGGGACATGAAACTTGTCCCCTACAAGATTGTGAACAAGGACGGTAAGCCCTACATTCAGGTCAAGATCAAGGATGGA

upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA05G36600.1 (Glycine max), 3'ss of exon 2
gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
|||| || |  |||     |||  || |    |||||  | |  |   |||  ||   ||| | || |  | ||        || |||    ||||| |  || |||||||| || || || ||||| |||||||| || |||||| ||||||||||||| || || ||||| ||||| || || |||||||| 
gtaagactta-ctcaagttgttgatttttgacaatttgatttgat---ggg--atcaatggtgactgacatgta-------tccttttgc--agGTTTGAAGATAAGGAAGTTCAAAGAGATATGAAGCTTGTTCCTTATAAGATTGTTAACAAGGATGGAAAACCTTACATACAGGTGAAAATTAAGGATGGT

upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA08G02940.1 (Glycine max), 3'ss of exon 2
gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
|||| || |  |||     |||  || |    |||||  | | ||   || | | || |  |    | ||||       ||  | ||| |  |||||||  || |||||||| || |  |||||||| |||||||| || |||||| | ||||||||||| || || ||||||||||| || || |||||||| 
gtaagacttg-ctcaagttgttgatttttgacaatttgatttggtg--ggatcaattgttac----tgactt-------gcatc-tttttgcagGTTCGAAGATAAGGAAGTTCAAAAAGACATGAAGCTTGTTCCTTATAAGATTGTCAACAAGGATGGAAAACCTTACATTCAGGTGAAAATTAAGGATGGT

upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA08G02960.1 (Glycine max), 3'ss of exon 2
gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
|||| || || |||      ||  ||  |   |||||  | | | ||||  | |     || |||| |  | ||        || |||    ||||| |  || |||||||| ||  | |||||||| |||||||| || |||||| | ||||||||||| || || ||||| ||||| || || |||||||| 
gtaagacatc-ctcaagtttttgatttaggacaatttgattt-gatgtgaataaa----ggtgtctgacatgta-------tccttttgc--agGTTTGAAGATAAGGAAGTTCAACGAGACATGAAGCTTGTTCCTTATAAGATTGTCAACAAGGATGGAAAACCTTACATACAGGTGAAAATTAAGGATGGT

upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: AT5G42020.1 (Arabidopsis thaliana), 3'ss of exon 2
gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatt-tattt---agGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
                  | |||||| |  |   |||  |||   | |  |||  |  ||   |  |   |||| |  |  |  ||| |||||   || || | ||||||||| || || | ||||| ||||||||| || ||| ||||| | ||||||||||| ||||| |||||||| ||||||||||||||||| 
----------------gtaattacttattctcttttttttctt-ctatatgtttgtgatgatattgttggtttaattctttcttattgtatttggcagATTTGAGGACAAGGAGGTTCAAAAGGACAGGAAACTTGTACCTTACCAGATTGTGAACAAGGATGGTAAGCCATACATTCAAGTCAAGATCAAGGATGGT

upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: AT5G28540.1 (Arabidopsis thaliana), 3'ss of exon 2
gtaacacctctctcctacagttacttctgttgaat-ttcgtctcgttgtgg-gttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
                  | |||||| |  |   | || || ||    ||| | |  |  ||  |  |||| | |  |  |     ||||    || || | ||| ||||| || || | ||| | ||||||||| || ||| ||||| | ||||||||||| ||||| |||||||| ||||||||||||||||| 
----------------gtaattacttattctctgtgtttgtttcaccttggagatgatattgttggtttaagtctt-tgttattgtatttggc-agATTTGAGGATAAGGAGGTTCAAAAGGATAGGAAACTTGTACCTTACCAGATTGTGAACAAGGATGGTAAGCCATACATTCAAGTCAAGATCAAGGATGGT

upper sequence: GRMZM2G114793_T01 (Zea mays), 3'ss of exon 2
lower sequence: Vv02s0025g02140.t01 (Vitis vinifera), 3'ss of exon 2
gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatt-tagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
|||| || ||| ||    |  ||   || |||   |  |  | ||  |  |||      |   | ||  ||   |   |    || || |   ||||| |  || ||||| || |||| ||||||||| |||||||| ||||| ||| ||||||||||||| ||||| |||||||| ||||||||||||||||| 
gtaatacgtctatct---atctaaagcttttggtctatgcattgt-attagttggcaaggaactttttgctgaggcttttaatcacttttgacagGTTTGAAGATAAGGAGGTTCAGAAGGACATGAAGCTTGTTCCGTACAACATTGTTAACAAGGATGGGAAGCCTTACATTCAAGTCAAGATCAAGGATGGT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148979002|gb|EE026432.2|EE026432
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGGACATGAAACTTGTTCCCCTACA-G
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGG-ACATGAAACTTGTTCCC-TACAAG
EST: gi|211043418|gb|FL322281.1|FL322281
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211399188|gb|FL334000.1|FL334000
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211287645|gb|FL366221.1|FL366221
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|149079422|gb|EE168400.2|EE168400
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|32843508|gb|CD983189.1|CD983189
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|148947395|gb|EC892803.2|EC892803
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211153937|gb|FL327665.1|FL327665
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211437934|gb|FL338200.1|FL338200
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAA
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAA
EST: gi|211537351|gb|FL321049.1|FL321049
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211272691|gb|FL347693.1|FL347693
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|32929450|gb|CF034262.1|CF034262
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211465040|gb|FL331928.1|FL331928
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAWA                         GTTCGCGGACAAGGAAGTC
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTC
EST: gi|211335770|gb|FL352931.1|FL352931
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTANCA-GA
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTA-CAAGA
EST: gi|148990654|gb|EE032048.2|EE032048
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211362047|gb|FL326991.1|FL326991
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|148957724|gb|EC903848.2|EC903848
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211100817|gb|FL317192.1|FL317192
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211198013|gb|FL335635.1|FL335635
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|211220897|gb|FL350387.1|FL350387
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
EST: gi|101399980|gb|EB821837.1|EB821837
EST:     GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAA                         GTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT
genomic: GTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 316

TCACCGATAGCGAGAGGCTCATCGGTGAGGCTGCCAAGAACCAGGCAGCCGTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 392

TCACCGATAGCGAGAGGCTCATCGGTGAGGCTGCCAAGAACCAGGCAGCCGTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 299

TCACCGATAGCGAGAGGCTCATCGGTGAGGCTGCCAAGAACCAGGCAGCCGTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 1401

TCACCGATAGCGAGAGGCTCATCGGTGAGGCTGCCAAGAACCAGGCAGCCGTCAACCCTGAGAGGACCATCTTTGACGTCAAGCGTCTCATCGGAAGAAAgtaacacctc ... atttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG
                        tcttaac  putative branch site (score: 1401)
                                         ccccattt  putative PPT
                          ttaactttagtaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaacacctctctcctacagttacttctgttgaatttcgtctcgttgtgggttatttctggcgtcttaactttagtaatgccccatttatttagGTTCGCGGACAAGGAAGTCCAGAGGGACATGAAACTTGTTCCCTACAAGATTATTAACAAGGATGGCAAGCCCTACATTCAGGTCAAGATCAAGGATGGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGATCA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGATG