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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatttggacgccagaacaatgtgctaacaacttcctgtttgccattaaaatgttcctccacagtaacagttattaattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT

Basic information

species Zea mays
transcript GRMZM2G104294_T02
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G104294_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os04g55960.1 (Oryza sativa), 3'ss of exon 8
gtatttggacgccagaacaatgtgcta---acaacttcctgtt-tgccattaaaatgt--------tcctccacagtaacagttattaattgct-agGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT
|||||   | | ||  | ||||   ||   ||| ||||||  | | |  ||| |||             || |    | || ||  | |||||  ||| |||||||||||||||||||||| || || || ||||||||||| |||||||| |||||||| ||||| ||||| || |||||| |||||||
gtattcatatgtcaagaaaatgatttagctacaccttccttctgttcagttagaatacagtatatgcattctagttcatcatttgattattgcccagGTCTAATGTTGCTGTTCGAAAGGAACTCCATAAGCCAGCTTCAGATCGCTCTTGCATTCATCTGGAGTTCGATATCTCAGGCACTGGTCTTGT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32850577|gb|CD990258.1|CD990258
EST:     ACTGGTGTGATTGATATTCACCACCCTTGCAG                         GGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTC
genomic: ACTGGTGTGATTGATATTCACCACCCTTGCAGgtatttggac ... taattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTC




















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 145

ACAGAGTTGTTTTCATTGATAAATCTGACCTGTCATTCCAAGATAGATCATGGTCACTAGCCAACGGTACTGGTGTGATTGATATTCACCACCCTTGCAGgtatttggac ... taattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 191

ACAGAGTTGTTTTCATTGATAAATCTGACCTGTCATTCCAAGATAGATCATGGTCACTAGCCAACGGTACTGGTGTGATTGATATTCACCACCCTTGCAGgtatttggac ... taattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 185

ACAGAGTTGTTTTCATTGATAAATCTGACCTGTCATTCCAAGATAGATCATGGTCACTAGCCAACGGTACTGGTGTGATTGATATTCACCACCCTTGCAGgtatttggac ... taattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 328

ACAGAGTTGTTTTCATTGATAAATCTGACCTGTCATTCCAAGATAGATCATGGTCACTAGCCAACGGTACTGGTGTGATTGATATTCACCACCCTTGCAGgtatttggac ... taattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aacttcctgtttgccattaaaatgttcctccacagtaacagttattaattgctagGGCTAATGTTGCTGTTCGAAAGGAGCTGCACAAACCAGCTTCAGACCGCTCTTGTATTCATCTTGAGTTTGATATTTCTGGCACTAGTCTTGT
                                          tattaat  putative branch site (score: 328)
                                   taacagttattaatt  TA-rich tract