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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtcaccatctcatcggaattttggttagacgtttgtttgtttttccctcatatggaatctcacttggttacctgtcttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC

Basic information

species Zea mays
transcript GRMZM2G093101_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G093101_T01 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g56880.1 (Oryza sativa), 3'ss of exon 2
gtcaccatctcatcggaattttggttagacgtttgtttgtttttccctcatatggaatctcacttggttacctgtcttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC
           ||  |   ||||   |     |  || || |||      ||   | |     |||  ||   |  || || |||||||||||||| || |||||||||||||||||||||||||| |||||||||||||||||    |||||||||||||| ||||||||||||||||||||| 
---------gtattagttctttgcaaaagtcgtctttagtgtttggactgta--aattaaagattgactattggctttgttgcagTTTGACACAAAATACTACTATGCTGTTGGGATTGGACAAACCGTGAGGAAGTTTTGGTTCAGAACCCCTCCAAAAAGTGGCCCAGATGTTCCATATACAT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|78110173|gb|DV528588.1|DV528588
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|76288888|gb|DV028456.1|DV028456
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|71438566|gb|DR819616.1|DR819616
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|211529301|gb|FL356735.1|FL356735
EST:     CAACTACACCTCAGGATACATTCATCATTGCACAATNNNNNNNGT-GGAG                         TTTGACACAAAGTATTACTATG
genomic: CAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAA-GTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATG
EST: gi|71439022|gb|DR820072.1|DR820072
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|71446702|gb|DR827752.1|DR827752
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|149085557|gb|EE175068.2|EE175068
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|148938422|gb|EC882740.2|EC882740
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|149094916|gb|EE180497.2|EE180497
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|149086647|gb|EE176188.2|EE176188
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
EST: gi|71312492|gb|DR792778.1|DR792778
EST:     ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAG                         TTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG
genomic: ACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 60

GGATAACCTCAACTATACTGCAAATGGGAAGCATACTCAGTATACTTTCTACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 43

GGATAACCTCAACTATACTGCAAATGGGAAGCATACTCAGTATACTTTCTACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 21

GGATAACCTCAACTATACTGCAAATGGGAAGCATACTCAGTATACTTTCTACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 50

GGATAACCTCAACTATACTGCAAATGGGAAGCATACTCAGTATACTTTCTACAACTACACCTCAGGATACATTCATCATTGCACAATAAAAAAGTTGGAGgtcaccatct ... cttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtttgtttgtttttccctcatatggaatctcacttggttacctgtcttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC
                          atctcac  putative branch site (score: 50)
                                     ttacctgtcttttttc  putative PPT
     tttgttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtcaccatctcatcggaattttggttagacgtttgtttgtttttccctcatatggaatctcacttggttacctgtcttttttcagTTTGACACAAAGTATTACTATGCTGTTGGGATTGGACAAACGGTGAGGAAGTTTTGGTTTTTGACCCCTCCAAAAAGCGGCCCAGATGTTCCATATACAC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGATG