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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtatgttcaacattctgttttctctcttgatgattttgttatttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT

Basic information

species Zea mays
transcript GRMZM2G090738_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G090738_T01 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g67126.1 (Oryza sativa), 3'ss of exon 2
gtatgttcaacattctgttttctctc--ttgatgattttgttatttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT
||| |||||| ||  |  ||||       | |||  ||   |  || |  |||  |||  || ||||||   ||  || ||  ||||| ||||||||||  || ||||| ||||||||||| ||||| ||| | |  |||||||| |||||||||||||| ||||| || ||||| ||  ||         
gtaagttcaatataatcctttcatgtggctcatgcattctgtgcttgtaatata-gctaggt-tttgatgtacttaatctcatttgttattgtttgcagAGGGCAAGACTGACTACAGGGCCAGGATTAGGCTCACCAACCAAGATAAGAACAAGTACAACACACCGAAGTACCGCTTCGTT---------

upper sequence: GRMZM2G090738_T01 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g67134.1 (Oryza sativa), 3'ss of exon 2
gtatgttcaacattctgttttctctcttgatgattttgttatttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT
               |   |    |      ||    |   |||         |    |  |||||    ||  || ||  ||||  ||||||||||  || ||||| ||||||||||| ||||| ||| | |  |||||||| |||||||||||||| ||||| || ||||| ||  ||||| |||||
--------------gtaagtcaagttcaagagaccgaggcgtttccaagtcaagtcaaggttttgacgtacttaatctcatttgtcattgtttgcagAGGGCAAGACTGACTACAGGGCCAGGATTAGGCTCACCAACCAAGATAAGAACAAGTACAACACACCGAAGTACCGCTTCGTTGTGAGATTT

upper sequence: GRMZM2G090738_T01 (Zea mays), 3'ss of exon 2
lower sequence: AT3G25520.1 (Arabidopsis thaliana), 3'ss of exon 2
---gtatgttcaacattctgttttctctcttgatgat-tttgttatttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT--------
    ||  ||    ||||  |||  |||  ||||  | |||||| | | |    ||   | ||| | ||| | |       | | || || ||||  | || ||| ||||| |||||||||||||||||  |  | || |||||||||||||||||||||||||| || || || || ||| |||| || |||        
gtaatagattgttgattcgttttattctgatgatattgtttgttctctctttgtttcaatctgtttctgaaaat-------tattttatttttgt--gtagATGGAAAGACTGACTACAGGGCAAGGATCCGTCTTATCAACCAAGACAAGAACAAGTACAATACTCCTAAGTATCGTTTTGTTGTCCGGTTTACCAACAA

upper sequence: GRMZM2G090738_T01 (Zea mays), 3'ss of exon 2
lower sequence: Vv05s0094g00870.t01 (Vitis vinifera), 3'ss of exon 2
gtat-gttcaacatt-ctgttttctctcttgatgattttgttatttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT
|||| || | ||||| |   | |   |  | ||  ||||    |||| |   |||| |  |  ||| || |  |  |||||   |    |||| | |||  || ||||| ||||||||||| |||||  |  ||||||| ||||||||||||||||| || || || ||||| || ||| |||||||||||
gtattgtactacatttccaatgttcattcttatagtttttgggttttcg---tttgtt-cgctttttatggatttaatttc---taaaattgtctacagAGGGAAAGACTGACTACAGGGCTAGGATTCGCCTGATTAATCAAGACAAGAACAAGTATAACACTCCGAAATATCGCTTTGTTGTGCGATTT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211279083|gb|FL069189.1|FL069189
EST:     GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTG                         CTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
genomic: GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
EST: gi|211279085|gb|FL069191.1|FL069191
EST:     GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTG                         CTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
genomic: GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
EST: gi|211279081|gb|FL069187.1|FL069187
EST:     GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTG                         CTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
genomic: GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
EST: gi|60672209|gb|DN474977.1|DN474977
EST:     GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTG                         CTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA
genomic: GACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 130

GGGGGGTTCGTCAAGACCCAGAAGACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 56

GGGGGGTTCGTCAAGACCCAGAAGACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 63

GGGGGGTTCGTCAAGACCCAGAAGACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 33

GGGGGGTTCGTCAAGACCCAGAAGACCAATGCGTACTTCAAGCGTTTCCAAGTGAAGTTCAAGAGAAGGCGTGgtatgttcaa ... ttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT
                  gtttgat  putative branch site (score: 33)
                                 tttctcttgttcttgt  putative PPT
                   tttgata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgttcaacattctgttttctctcttgatgattttgttatttttgctatttgctgtgtgtttgatagtctacatttctcttgttcttgtttgcagCTGGTAAGACAGACTACAGGGCAAGGATAAGGTTGATTAACCAAGACAAGAACAAGTACAATACACCCAAATACCGATTTATTGTGCGATTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAC