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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaacatccttactctttcgaaccgaccctccgcctgcctttcacatgccctaattcgcggaatcaaaaaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG

Basic information

species Zea mays
transcript GRMZM2G083346_T01
intron # 2
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|148956260|gb|EC902057.2|EC902057
EST:     TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCA                         CTTTCACTTGGACCGCGCCGCATCGCTGCCGTACTTCCTGGAGAAG
genomic: TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
EST: gi|149087996|gb|EE177212.2|EE177212
EST:     TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCA                         CTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
genomic: TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
EST: gi|148955806|gb|EC901491.2|EC901491
EST:     TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCA                         CTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
genomic: TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
EST: gi|71420449|gb|DR805097.1|DR805097
EST:     TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCA                         CTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
genomic: TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
EST: gi|211114031|gb|FL096536.1|FL096536
EST:     TACTTCGACGAAATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCA                         CTTTCACTTGGACCACGCCGCATCGCTACCGTACTTCCTGGAGAAG
genomic: TACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 43 (upstream exon), 46 (downstream exon)
Score: 22

TTCGACTGCGGCATCCACCCGGCGTACACCGGCATGGCGGCGCTGCCCTACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG


Block sizes: 45 (upstream exon), 46 (downstream exon)
Score: 8

TTCGACTGCGGCATCCACCCGGCGTACACCGGCATGGCGGCGCTGCCCTACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG


Block sizes: 48 (upstream exon), 30 (downstream exon)
Score: 6

TTCGACTGCGGCATCCACCCGGCGTACACCGGCATGGCGGCGCTGCCCTACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 8

TTCGACTGCGGCATCCACCCGGCGTACACCGGCATGGCGGCGCTGCCCTACTTCGACGAGATCGATCCGTCCGCCATAGACGTCCTCCTCATCACCCAgtaacatcct ... aaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cgaccctccgcctgcctttcacatgccctaattcgcggaatcaaaaaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG
                         ccctaat  putative branch site (score: 8)
                                      aatcaaaaaaatca  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaacatccttactctttcgaaccgaccctccgcctgcctttcacatgccctaattcgcggaatcaaaaaaatcacagCTTTCACTTGGACCACGCCGCATCGCTGCCGTACTTCCTGGAGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAA