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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...aaatgttactgatgttgataagctgtgatgcttgctggattcactgtagcatttatgctataatcttctgatgtttccttcccctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA

Basic information

species Zea mays
transcript GRMZM2G032267_T02
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G032267_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os07g08170.1 (Oryza sativa), 3'ss of exon 2
aaatgttactgatgttgataagctgtgatgcttgctgga---ttcactgtagcatttatgctat----aatcttctgatgtttccttcccctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
     | | ||   |  ||  ||| | ||  ||| |  |   | || |||  |||| ||| |       |   ||| |||| |  |     | |   | | || |||||||| ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| ||||| ||||||||||||||||||||||||||||
-----tcaatgtcttacatctgct-tcatttttggtaaaacttccaatgtttcattcatgttgatggagaatatcttatgtgttatcttattatt-ttactatacagGTCCCTTTCTGTCCTATGGTAGGATCAGAAGTGTATTCCTCAGAGGTCAAGAAAACTGAGGTGCTAATGGAGAATTTCCGTAGAGCTATAGGTTTGCGTA

upper sequence: GRMZM2G032267_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g62040.3 (Oryza sativa), 3'ss of exon 2
--aaatgttactgatgttgataagctgtgatgcttgctggattcactgtagcatttatgctataatcttctgatgtttccttcccctgtcactgc--aatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
  |   |||  |  | |||||   ||   |    | ||  | |    |||  | ||    | ||       | || |   ||  | ||| | | |  ||  |||||||| |||||||||||||| |||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||| |||||||||| |||||||
ttatgagttttttgt-ttgatgccct---acatctacttcagttttggtaaaagttccaatgtatctaatggttgatatgttgtcttgttatttcataacacagGTCCCCTTCTGTCCTATGGTGGGATCAGAAGTGTATTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTCGAGCTATAGGCTTGCGTA

upper sequence: GRMZM2G032267_T02 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA07G02170.1 (Glycine max), 3'ss of exon 2
-aaatgttactgatgttgataagct-gtgatgcttgctggat-tcactgtagcatttatgctataatcttctgatgtttccttcccctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
       |    |   |   |  || ||| || ||  |   | ||  |||     || ||        | ||| || |         | |  |  |  ||||||| || ||||| || ||||| || || ||||| ||||||||||| || ||||| || ||||| || |||||||||||  |  | || || ||| ||||||
gtttccctctcaagactctcattctcgtggtgttttttttttctctgtgttttgattgtgacccttcttgctgctgataataatgtttat-gccactctgcagGTTCCTTTCTGCCCAATGGTTGGTTCTGAAGTTTACTCCTCAGAAGTAAAGAAGACCGAGGTTCTCATGGAAAATTTTAGACGGGCCATTGGTCTGCGTA

upper sequence: GRMZM2G032267_T02 (Zea mays), 3'ss of exon 2
lower sequence: GLYMA08G21830.1 (Glycine max), 3'ss of exon 2
aaatgttactgatgttgataagctgtgatgcttgctggattcactgtagcatttatgctataatcttctgatgtttccttcccctgtcactgcaat--gcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
             |||  |       ||  ||      ||||  |||    |   || ||     |  || || ||          | | | || |  |||||| || ||||| |||||||| || || ||||| |||||||| || || ||||| |||||||| || ||||||||||| ||  | || || ||| ||||||
-------------gtttctttctcaagactctc-----attctttgtgttgtgattgttaccccttcttgctgcttataattaagtttattccactctgcagGTACCGTTCTGCCCTATGGTTGGCTCCGAAGTTTACTCCTCGGAAGTAAAGAAGACTGAGGTTCTCATGGAAAATTTTCGACGGGCCATTGGTCTGCGTA

upper sequence: GRMZM2G032267_T02 (Zea mays), 3'ss of exon 2
lower sequence: AT5G22330.1 (Arabidopsis thaliana), 3'ss of exon 2
--------aaatgttac----tgatgttgataagctgtgatgcttgc---tggattcactgtagca---tttatgctataatcttctgatgtttccttccc--ctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
          ||||  |    ||    |  |  | |    || || |   ||| |||| ||||  |   ||| | || | |  ||  | |  ||   ||     |||  ||    ||||||| ||||||||||| ||||| ||||| || || ||||| |||||||| |||||||| ||||| || ||||| |||||  |  | || || ||| | ||||
gtaattttggatgtctcattttgtatcttctttgttacattgtttccacatggcttcattgtatgaagcttttttcttttacttttagcttattttatctggattgtttctttcttgcagGTTCCATTCTGTCCAATGGTTGGATCTGAGGTTTACTCATCAGAGGTTAAGAAAACAGAGGTTCTCATGGAGAATTTTAGACGTGCCATTGGTCTACGTA

upper sequence: GRMZM2G032267_T02 (Zea mays), 3'ss of exon 2
lower sequence: Vv06s0004g02910.t01 (Vitis vinifera), 3'ss of exon 2
--------------------------------------------------------------------aaatgttactgatgttgataagctgtgatgcttgctggattcactgtagcattt---atgctataatcttctgatgttt-----ccttcccctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
                                                                    | || |   |  ||  | |       | || ||    |  |  || | | || |   ||||  || ||||| |  || |     |       |    |||   | ||||| || ||||| || ||||| |||||||| || ||||| |||||||| |||||||||||| |  | ||||||||||||||  | ||||| ||| | ||||
gtacaacttaaatgtactcttaccattttttgttcagtagtgtcttgaattctctttatcagagctgtagatttagattctggggttctctccagttgattttcagtctagctctgggatgttgaatgccttactcttcaggggtgtgagatctgctttttacttctgatttacagGTTCCTTTCTGCCCAATGGTGGGATCAGAGGTATACTCATCAGAGGTAAAGAAAACTGAGATTTTAATGGAAAATTTCCGACGGGCTATTGGTCTACGTA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|8102879|gb|AW927557.1|AW927557
EST:     CGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAG                         GTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAG
genomic: CGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAG
EST: gi|8102878|gb|AW927556.1|AW927556
EST:     CGGGCAAAACGGCGCTAGCGCTCGGCATATTGCAGGAGCTCGGCAGCAAG                         GTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAG
genomic: CGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAG
EST: gi|78103432|gb|DV521860.1|DV521860
EST:     CGCAGGAGCTCGGCAGCAAG                         GTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAG
genomic: CGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 214

CCAGAAGAAGATGGCCGGTCGCGCGCTGCTCCTTGCGGGCCCGCCCGCCACGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 74

CCAGAAGAAGATGGCCGGTCGCGCGCTGCTCCTTGCGGGCCCGCCCGCCACGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 97

CCAGAAGAAGATGGCCGGTCGCGCGCTGCTCCTTGCGGGCCCGCCCGCCACGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 93

CCAGAAGAAGATGGCCGGTCGCGCGCTGCTCCTTGCGGGCCCGCCCGCCACGGGCAAAACGGCGCTAGCGCTCGGCATAGCGCAGGAGCTCGGCAGCAAGgtccgtgcgc ... tgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtagcatttatgctataatcttctgatgtttccttcccctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA
                    ttctgat  putative branch site (score: 93)
                            tttccttcccctgtca  CT-rich tract
     atttatgctataat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
aaatgttactgatgttgataagctgtgatgcttgctggattcactgtagcatttatgctataatcttctgatgtttccttcccctgtcactgcaatgcagGTCCCATTCTGTCCTATGGTAGGATCAGAAGTGTACTCCTCAGAGGTCAAGAAAACTGAGGTGCTGATGGAAAATTTCCGTAGAGCTATAGGTTTGCGTA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GATGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCT