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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tcatcacatttcttttctgctttcctaattttctcgaatgcttctttgcaaatacaccatggattaggattatctgtgggtttcaatcttttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG

Basic information

species Zea mays
transcript GRMZM2G025366_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G025366_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os04g40310.2 (Oryza sativa), 3'ss of exon 2
tcatcacatttcttttctgctttcctaattttctcgaatgcttctttgcaaata-caccatggatta---ggattatctgtgggtttcaatctttt------ttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG
           |||| |||  | | |  ||||| | |||| ||| |   || || | || || | |    | |  || | |   |||| |   |||      ||||||||||||| || || ||||||||||||||||| |||||||||||||||||||||| ||||||||| ||  | ||||||||||
---------cactttcctgaatac-tggttttcccaaatgattcctgttaagtatccccgtgtaattcctgcagaatttctaactttctactctttggtacgttttgtagGTTACACCAAACCTTTATGGCAATTTGGTTGCTAACACGGCTGCAGGTATTGCTGGAGGGACAGGTGTTATGCCTGGAG

upper sequence: GRMZM2G025366_T02 (Zea mays), 3'ss of exon 2
lower sequence: AT4G35260.1 (Arabidopsis thaliana), 3'ss of exon 2
tcatcacatttcttttctgctttcctaattttctcgaatgcttctttgcaaatacaccatggattaggattatctgtgggtttcaatcttttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG
 | ||||  ||  |   |  |  |||    ||   | |||       |    |   |  |         ||||    |   || |     | ||||| |||| ||||| ||||| ||||| ||||| || || ||||| ||||| ||||||| |||||| || ||  ||||||||||||
acttcactgttgctaagtattaacctttcattactgtatgtagtagcgtcttttggcttttccccccttttataacagcaatttatgaaatgttttgcagGTAACGCCGAATCTCTATGGGAATTTAGTTGCAAACACTGCTGCTGGTATTGCTGGAGGCACAGGAGTCATGCCTGGAG

upper sequence: GRMZM2G025366_T02 (Zea mays), 3'ss of exon 2
lower sequence: AT2G17130.2 (Arabidopsis thaliana), 3'ss of exon 2
tcatcacatttcttttctgctttcctaattttctcgaatgcttctttgcaaatacaccatggattaggattatctgtgggtttcaatcttttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG
             |   || ||| |     || | || |||||    || |     |      || || |||| |    || ||      |  |||| |||| ||||||||||| ||||| ||| | ||||| ||||| ||||| ||||||| |||||| || ||  ||||||||||||
------------gtaaatgtttttcagcacttgttga-tgcttg--ggctagcttccttctattttggtttatttacttgtatc------tgctttgcagGTGACGCCTAATCTCTATGGTAATCTAGTGGCAAACACTGCTGCTGGTATTGCTGGAGGTACTGGAGTCATGCCTGGAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|149089013|gb|EE178164.2|EE178164
EST:     NCA-CTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATG                         GTTACGCCTAATCTTTATGGCCATTTTGGTGGCTAACACGGCTGCAGGTAT
genomic: -CAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTT-GGTGGCTAACACGGCTGCAGGTAT
EST: gi|148959186|gb|EE010640.2|EE010640
EST:     ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATG                         GTTACGCCTAATCTTTATGGCAATTTGGGGGCTAACACGGCTGCAGGTATT
genomic: ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATT
EST: gi|148959185|gb|EC905302.2|EC905302
EST:     ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATG                         GTTACGCCTAATCTTTA
genomic: ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTA
EST: gi|5901228|gb|AW042328.1|AW042328
EST:     AACTGCTCTATGCAACTTGTTTCAAAGGCCTGAACAATTTGATGGTCATG                         GTTACGCCTAATCTTTATGGCAATTTG
genomic: AACTGCTCTATGCAACTTGTTTCAAAG-CCTGAACAATTTGATG-TCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTG
EST: gi|76285692|gb|DV025260.1|DV025260
EST:     ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATG                         GTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATT
genomic: ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATT
EST: gi|76285693|gb|DV025261.1|DV025261
EST:     ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATG                         GTTACGCCTAATCTTTATGGCANTTTGGTGGCTAACACGGCTGCA
genomic: ACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 48

TGAAGTTGCTTCAAAATATCCTGGTATTCAGTACAATGAAATGATTGTTGACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 99

TGAAGTTGCTTCAAAATATCCTGGTATTCAGTACAATGAAATGATTGTTGACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 84

TGAAGTTGCTTCAAAATATCCTGGTATTCAGTACAATGAAATGATTGTTGACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 175

TGAAGTTGCTTCAAAATATCCTGGTATTCAGTACAATGAAATGATTGTTGACAACTGCTCTATGCAACTTGTTTCAAAGCCTGAACAATTTGATGTCATGgtatgtatct ... ttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttgcaaatacaccatggattaggattatctgtgggtttcaatcttttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG
                                   tttcaatctttttttt  CT-rich tract
                                   tttcaatctttttttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tcatcacatttcttttctgctttcctaattttctcgaatgcttctttgcaaatacaccatggattaggattatctgtgggtttcaatcttttttttgtagGTTACGCCTAATCTTTATGGCAATTTGGTGGCTAACACGGCTGCAGGTATTGTTGGAGGGACGGGCATCATGCCTGGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGG