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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...cgtggttcacctcagtgtcttttattggcagcagaacaaaccgtctgctttctgattttaactgtcagtttgctaacatatgtcaagtttacacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA

Basic information

species Zea mays
transcript GRMZM2G007260_T03
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G007260_T03 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os03g57790.1 (Oryza sativa), 3'ss of exon 2
-------cgtggttcacctcagtgtcttttattggcagcagaacaaaccgtctgctttctgattttaactgtcagtttgctaacatatgtcaagtttacacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA
       | | | |         || | || |  |  |    |  |    |   | ||   |||| |  | | |||| |     ||| || |  ||  |||   ||||||||||||||||||| || ||||||||||||||||| ||||||||||| ||   |||||||||||| || ||||||||||||||||| |
aaaatcacatcgattgaaatgaagtatgtttt--gtggtgttattacttatgaacatt---atttcatgtttaagttagtactaataagtgattttgtcac--atagGCACGTTCAAGCTGACACTACAATTTACAGAAGATTATCCCAACAAACCACCAGTTGTTCGGTTTGTCTCAAGGATGTTTCACCCAAATA

upper sequence: GRMZM2G007260_T03 (Zea mays), 3'ss of exon 2
lower sequence: PP1S219_106V6.1 (Physcomitrella patens), 3'ss of exon 2
cgtggttcacctcagtgtctt-ttattggcagcagaac----aaaccgtctgctttctgattttaactgtcagtttgctaacatatgtcaagtttacacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA
           || || | |   |  |||     ||||    |   |  |||  |||||    | | |||  |  || ||  ||  |    |||| |   |  ||| ||||||||| ||||  |||||||||| || ||||| || ||||| ||||| || || || ||||| |||| |||||||||||| ||||
nnnnnnnnnngtccgtttttgacttctggggattgaacgtttagggctactgtgttctgtgcctca-tgtgtgcatgttactatgcg----gttttcttcttcagGGACGTTCAAGTTGACGTTGCAATTTACCGAGGATTATCCGAACAAGCCTCCTACAGTGCGTTTTGTCTCGAAGATGTTTCACCCTAACA

upper sequence: GRMZM2G007260_T03 (Zea mays), 3'ss of exon 2
lower sequence: EFJ17802 (Selaginella moellendorffii), 3'ss of exon 2
cgtggttcacctcagtgtcttttattggcagcagaacaaaccgtctgctttctgattttaactgtcagtttgctaacatatgtcaagttt------acact-tgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA
                                 |            |||||  ||||||  |      ||||||  |  |  |  || ||       |||| |  ||| ||||| ||||||||  ||||||| ||||| || || |||||||||||||| || ||  |||||||||| | |||||| || || || |
---------------------------------gtgaggttttagagcttttggatttttccgtgtgttttgcttgctaagcttgagcttttggtggcactgtacagGGACGTTTAAGCTGACATTGCAATTCACAGAGGACTATCCCAACAAACCTCCCACCGTGAGGTTTGTTTCCAAGATGTTCCATCCCAATA

upper sequence: GRMZM2G007260_T03 (Zea mays), 3'ss of exon 2
lower sequence: EFJ16328 (Selaginella moellendorffii), 3'ss of exon 2
cgtggttcacctcagtgtcttttattggcagcagaacaaaccgtctgctttctgattttaactgtcagtttgctaacatatg-tcaagtttacact-tgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA
                           |         | | | | ||  || |     |   | |  |||    | | || | |   | |  |||| |  ||| ||||| ||||||||  ||||||| ||||| || || |||||||||||||| || ||  |||||||||| | |||||| || || || |
---------------------------gtgaggttttagagcttttggattttt-ccgtgtgttttggttgctaagcttaagcttttggtggcactgtacagGGACGTTTAAGCTGACATTGCAATTCACAGAGGACTATCCCAACAAACCTCCCACCGTGAGGTTTGTTTCCAAGATGTTCCATCCCAATA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71448180|gb|DR829230.1|DR829230
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|22521069|gb|BU079880.1|BU079880
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|20803162|gb|BQ294212.1|BQ294212
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211160852|gb|FL355935.1|FL355935
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|37377294|gb|CF625238.1|CF625238
EST:     CGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: CGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209638|gb|FL170006.1|FL170006
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211158663|gb|FL318093.1|FL318093
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|94474209|gb|EB703167.1|EB703167
EST:     CGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: CGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|89759914|gb|DY688576.1|DY688576
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|74241480|gb|DT649394.1|DT649394
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|50332498|gb|CO527624.1|CO527624
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|74241479|gb|DT649393.1|DT649393
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211212202|gb|FL170018.1|FL170018
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|44900351|gb|CK826896.1|CK826896
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|4624364|gb|AI615197.1|AI615197
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|71299516|gb|DR785767.1|DR785767
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|7031256|gb|AW461144.1|AW461144
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209646|gb|FL170014.1|FL170014
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211416709|gb|FL320472.1|FL320472
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|71299514|gb|DR785766.1|DR785766
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTTTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209634|gb|FL170002.1|FL170002
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|149112233|gb|EE191289.2|EE191289
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|60401065|gb|DN233872.1|DN233872
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209637|gb|FL170005.1|FL170005
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|12972475|gb|BG268000.1|BG268000
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|148966066|gb|EE017005.2|EE017005
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211212200|gb|FL170016.1|FL170016
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|18168064|gb|BM337904.1|BM337904
EST:     CGGGCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: CGGGCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209639|gb|FL170007.1|FL170007
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209642|gb|FL170010.1|FL170010
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|94474208|gb|EB703166.1|EB703166
EST:     CGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: CGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|13149803|gb|BG320125.1|BG320125
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|40337033|gb|CK371103.1|CK371103
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACATAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|149112232|gb|EE191288.2|EE191288
EST:     GATGACACGCCGTGGG-CGGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCT
genomic: GATGACACGCCGTGGGAC-GGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCT
EST: gi|37397476|gb|CF636037.1|CF636037
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACATAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211212201|gb|FL170017.1|FL170017
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|18168754|gb|BM338594.1|BM338594
EST:     CGGGCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: CGGGCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211209644|gb|FL170012.1|FL170012
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|14701443|gb|BI233861.1|BI233861
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|50332499|gb|CO527625.1|CO527625
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
EST: gi|211500859|gb|FL344778.1|FL344778
EST:     GCCGGATGACACGCCGTGGGACGGAG                         GCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC
genomic: GCCGGATGACACGCCGTGGGACGGAGgtgagaccca ... acacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTC


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgctttctgattttaactgtcagtttgctaacatatgtcaagtttacacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA
                         tgctaac  putative branch site (score: 1)
       tgattttaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cgtggttcacctcagtgtcttttattggcagcagaacaaaccgtctgctttctgattttaactgtcagtttgctaacatatgtcaagtttacacttgtagGCACGTTCAAGCTGACTCTGCAATTTACAGAAGATTACCCCAACAAACCTCCGACTGTTCGGTTTGTTTCGAGGATGTTTCACCCAAACA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGGATG