Skip to another splice site:
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 5'  3'   
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 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...acagtgtctgcaacgctgtccgtgcttaacaatttgttagattttggatgatgacgaacgatcatgtgcttaacacgacagcaaaaacccgtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG

Basic information

species Zea mays
transcript GRMZM2G004161_T03
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G004161_T03 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os01g68020.1 (Oryza sativa), 3'ss of exon 2
acagtgtctgcaacgctgtccgtgcttaacaatttgttagattttggatgatgacgaacg-atcatgtgcttaacacgacagcaaaaacccgtctgggcag-
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---taacttggaccacaaaacgtgtagtagaaat------atttgagaaaaaaacatttgcatattagacgtgctaatactgtgtaaattttgttaactaattgtgcagTGGTGATGAGGAGGAGATTGACGAGAAGAGCGCGGCGCAGATGCTGGTGCTGGCGCACGCGTACCGGGTGCCGTGGCTGAAGCGGCGGTGCGAGGGGGCG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76284805|gb|DV024373.1|DV024373
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|91875248|gb|EB405205.1|EB405205
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|76292352|gb|DV031920.1|DV031920
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|50327672|gb|CO522798.1|CO522798
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|94477387|gb|EB706345.1|EB706345
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|101389281|gb|EB816595.1|EB816595
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|148992171|gb|EE033547.2|EE033547
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|89250799|gb|DY622585.1|DY622585
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
EST: gi|76918629|gb|DV167383.1|DV167383
EST:     GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAG                         GTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT
genomic: GTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 37 (downstream exon)
Score: 18

AGGACAGGGAGAGCGGCAAGCCCGGGCGGTCCGTCGTCCGGATCCGCGGCGTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 80

AGGACAGGGAGAGCGGCAAGCCCGGGCGGTCCGTCGTCCGGATCCGCGGCGTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 72

AGGACAGGGAGAGCGGCAAGCCCGGGCGGTCCGTCGTCCGGATCCGCGGCGTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG


Block sizes: 24 (upstream exon), 28 (downstream exon)
Score: 2

AGGACAGGGAGAGCGGCAAGCCCGGGCGGTCCGTCGTCCGGATCCGCGGCGTCACCGACGACGCCGCCGCGGCCTTCGTCCGCCTCCTCTACGCCGGCAGgtaacgcccg ... gtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ggatgatgacgaacgatcatgtgcttaacacgacagcaaaaacccgtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG
                      gcttaac  putative branch site (score: 2)
 cccgtct  putative PPT
 aaaaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
acagtgtctgcaacgctgtccgtgcttaacaatttgttagattttggatgatgacgaacgatcatgtgcttaacacgacagcaaaaacccgtctgggcagGTGCGGCGAGGATGAGGATGACGACATGGAGGAGCACGCGGTGCAGGTGCTGGTGCTGGCGCACGCGTACCAGGTGCCGTGGCTGAAGCGGGCGTGCGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC