ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

gtaagttgactctgaacaacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT

Basic information

species	Zea mays
transcript	GRMZM2G113202_T01
intron #	18
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: LOC_Os10g34710.1 (Oryza sativa), 3'ss of exon 18

gtaagttgact--ctgaacaacggcaaaaaaattct-gcaaccttttttt----aaaagt-taatacttaatttaatcagGTATGGTTTTCTTCT---TGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
|||| |  | |   ||||||   | |    | |||| |  ||||||| ||    || | | ||| | || |||| ||||||||||| ||||||||   ||||||||| ||||||||||| ||||| |||||||| |||||||||||||| ||||| |||||||| |||||||||||||||   
gtaaataaaatatttgaacagtagtattgcatttctagttaccttttattttgcaatactgtaacagttcattttatcagGTATGGCTTTCTTCTTCTTGAGGATGTCGCATCTCAAGACCCACTTAGTGTTTCGGTTGCAATTCTTCACCAGTTTAACATTTTGCCTGAGATGTATCAA---

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: GLYMA20G23660.1 (Glycine max), 3'ss of exon 19

--------------------------------------------------------gtaagttgactctgaacaacgg----caaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCT---TGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
                                                        ||    |  | |  | |  ||          | |||    | | || | |||     |      |  |  |||||| ||||||||||||||   |||  ||||||| ||||| |||||||| |||||||||||||| ||||||||||| ||||||||||| |||||||||||||||   
gtataacaacagagaaagaaactatggtgattgatatcctttcaaattacccccccgtcccctatcccctatccccgtgctttttgcacatttctaacctttctgttattgacttgggagtggta-aatcagATATGGTTTTCTTCTCCTTGAAAATGTTGCCTCTCAGGATCCACTTAGTGTTTCAGTTGCTATTCTTCATCAATTCAACATTTTGCCTGAGATGTATCAA---

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: GLYMA10G43230.1 (Glycine max), 3'ss of exon 17

-------------------------------------gtaagttgactctgaac--aacggcaaaaaaat-tctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCT---TGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
                                       || ||    |  | |  |  |        |  | |  ||| ||| | | |  | |          | |||||| ||||||||||||||   |||  ||||||| ||||| |||||||| |||||||||||||| ||||||||||||||||||||||| |||||||||||||||   
gtagaacaacaaagaaactatggtgattgatatcctttcaaattagtccccatcctagtgttttttgcacgtttctaacttttctgttattgatttgggagcggtgaatcagATATGGTTTTCTTCTCCTTGAAAATGTTGCCTCTCAGGATCCACTTAGTGTTTCAGTTGCTATTCTTCATCAGTTCAACATTTTGCCTGAGATGTATCAA---

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: GLYMA09G42180.1 (Glycine max), 3'ss of exon 17

---------------------------------------------------gtaagttgactctgaacaacggcaaaaaaat-tctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCT---TGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
                                                     |  ||   ||||  |    |     | || | |  | |||| || | |  |       |   || |||||| ||||||||||| ||   |||  ||||||| ||||| ||||| || ||||||||||||||||||||||||||||| || ||||| |||||||||||||||   
gtataataacagaaaactgatgatttatatcctttcaaatactatcaccttccacattccttctgccctggtgtttttacatgtgtctatccttgttcttattggcttgggtgtgttgaatcagATATGGTTTTCTCCTCCTTGATCATGTTGCTTCTCAGGATCCGCTTAGTGTTTCAGTTGCAATTCTTCATCAGTTTAATATTTTGCCTGAGATGTATCAA---

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: GLYMA20G00320.1 (Glycine max), 3'ss of exon 19

----------------------------------------------------gtaagttgactctgaacaacggcaaaaaaat-tctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
                                                      |  ||  ||| |  |    |       || | |  |||||| || | |  |       |  ||| |||||| ||||||||||| |||||  ||||||||||||| |||||||| |||||||| |||||||||||||||||||| || |||||  | |||||||||||||||
gtataacgacagaaagactgataatttatatcctttcaaatactatcaccttccacattccctccgccctggtgtttttgcatgtgtctaaccttgttcttattggcttgggtgcattgaatcagATATGGTTTTCTCCTTGATAATGTTGCATCTCAGGATCCACTTAGTGTTTCGGTTGCAATTCTTCATCAGTTTAATATTTTGTCGGAGATGTATCAAGTT

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: AT3G19960.1 (Arabidopsis thaliana), 3'ss of exon 19

--------gtaagttgactctgaacaacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatca-------gGTATGGTTTTCTTCTTG---AGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
        |  | |   |  |       |   ||   |   | ||| | ||| || | |  |  || | |  || ||||       | |||||||||||||| |   || |  ||||   |  |||||| || |||||||||||||| ||||| |||||||| || || || || ||||||||||||   
gtgacaatgccattcatcattagtagttgtgcaagggaccgtacaagcgtttcttcataagttttaatcacattgatcatattgcagATATGGTTTTCTTCTGGTGGAGAACATTGCTGATAGAGATCCTCTAAGTGTTTCAGTTGCGATTCTCCATCAGTTTAATATCTTGCCGGAGATGTATCAA---

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: AT3G19960.2 (Arabidopsis thaliana), 3'ss of exon 22

gtaagttgactctgaacaacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTTG---AGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT---------------------------------
                                            ||| ||    | |  | |  ||   ||| |||||||||||||| |   || |  ||||   |  |||||| || |||||||||||||| ||||| |||||||| || || || || |||||||||||||||                                 
-------------------------------------------gttttaatcacattgatcatattg--cagatatggttttcttctggtggagaacattgctgatagagatcctctaagtgtttcagTTGCGATTCTCCATCAGTTTAATATCTTGCCGGAGATGTATCAAGTTGGCTACACAAAGTTGTTTTTCAGAACTGGCCAG

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: AT1G50360.1 (Arabidopsis thaliana), 3'ss of exon 19

-------------------------------------------------------gtaagttgactctgaacaacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTT---GAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
                                                        || | |  |  |        |    || | | |  ||   |  |       ||  |   | ||    | ||||||||||| |||||    ||| |  ||||| |  ||||||| || |||||||| ||||||||||| |||||||| || || || || |||||||| |||   
gtaacaactccatctccagttggtgcactttcattgtattgcatgtcctcttataataggcttcccatactttttagttgcaataatgttgaatggtcatgctgtttttcttttgtcatact-ttagGTATGGTTTCCTTCTGCTGGAGAACATTGCAGCAAAAGATCCTCTAAGTGTTTCGGTTGCAATTCTCCATCAGTTTAATATCTTGCCAGAGATGTACCAA---

upper sequence: GRMZM2G113202_T01 (Zea mays), 3'ss of exon 18
lower sequence: Vv10s0071g00210.t01 (Vitis vinifera), 3'ss of exon 18

------------------------------------------------------gtaagttgactctgaacaacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTT---GAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT
                                                      |||   |     | |  | | |     |  || |   |  ||   ||     ||  |  ||||||  | ||||||||||||||||||    |||| ||||||||||||||||||||| ||||||||||||||||| |||||||||||||| ||| | |||||||||||||||   
gtacacatagatgacaaggttttattatctagttgtattgcacttataaattttgtattcttttcttaagaatctgtttttattttattttattttcccttgt---ttggttgttaatt-gaccagGTATGGTTTTCTTCTACTGGAGGGTGTTGCATCTCAAGATCCACTTAGTGTTTCAGTTGCAATCCTTCATCAGTTCAATATTCTGCCTGAGATGTATCAA---

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|76924723|gb|DV170102.1|DV170102

EST:     TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCG                         GTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC
genomic: TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCGgtaagttgac ... atttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC

EST: gi|32934067|gb|CF038879.1|CF038879

EST:     TCAAGATCTGGGTATCCAACAAGAATGGCTCACCAGAAGTTTGCTCGGCG                         GTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC
genomic: TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCGgtaagttgac ... atttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC

EST: gi|211293264|gb|FL097156.1|FL097156

EST:     TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCG                         GTATGGTTTTCTT
genomic: TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCGgtaagttgac ... atttaatcagGTATGGTTTTCTT

EST: gi|32935299|gb|CF040111.1|CF040111

EST:     TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCG                         GTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC
genomic: TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCGgtaagttgac ... atttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC

EST: gi|74243522|gb|DT651436.1|DT651436

EST:     TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCG                         GTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC
genomic: TCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCGgtaagttgac ... atttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 28 (downstream exon)
Score: 206

TTGTGCTTCAACAACTTAAATGTTGTGGGGTTCTTGAGGTTGTCCGGATTTCAAGATCTGGGTATCCAACAAGAATGACTCACCAGAAGTTTGCTCGGCGgtaagttgac ... atttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT

Block sizes: 49 (upstream exon), 28 (downstream exon)
Score: 128

Block sizes: 49 (upstream exon), 28 (downstream exon)
Score: 113

Block sizes: 49 (upstream exon), 28 (downstream exon)
Score: 194

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

aacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT

                                        acttaat  putative branch site (score: 194)
                   aacctttttttaaaag  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagttgactctgaacaacggcaaaaaaattctgcaacctttttttaaaagttaatacttaatttaatcagGTATGGTTTTCTTCTTGAGGATGTTGCATCTCAAGATCCACTCAGTGTTTCAGTTGCAATTCTTCATCAGTTCAACATTTTACCTGAGATGTATCAAGTT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GAGATG