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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgaatcttttatcctcagcatatctgaagctggaaactggaatgcttggtgtaattcattatccattcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC

Basic information

species Zea mays
transcript GRMZM2G171961_T01
intron # 17
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G171961_T01 (Zea mays), 3'ss of exon 17
lower sequence: LOC_Os06g20500.1 (Oryza sativa), 3'ss of exon 16
gtgaatcttttatcctcagcatatctgaa-gctggaaactggaatgcttggtgtaattcatta-tccattcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC
     |  | |  ||| |    || | || | |||| |||||| |  ||    || || |||| |||| |   |  |||||||||| |||||| ||||||||||| || ||||| || ||| |||| |||||||| || |||| ||||||||||||||||||||||||||| ||||| |
----gtaatattgcctaaattcatttaaaagatggagactggagtcgttttcttattttattaatccactaaaaaacagATTAGTATATTCCCCTCAAAAGAATTCTACGAAGGTGTCCTAGAAGACGGGGAGGGACTCAGCAAGAAACGTCCATGGCATTCCTACAGCTGTTTCGGCC

upper sequence: GRMZM2G171961_T01 (Zea mays), 3'ss of exon 17
lower sequence: LOC_Os03g32526.1 (Oryza sativa), 3'ss of exon 16
gtgaatcttttatcctcagcatatctgaa-gctggaaactggaatgcttggtgtaattcatta-tccattcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC
     |  | |  ||| |    || | || | |||| |||||| |  ||    || || |||| |||| |   |  |||||||||| |||||| || |||||||| || ||||| || ||| |||| |||||||| || |||| ||||||||||||||||||||||||||| ||||| |
----gtaatattgcctaaattcatttaaaagatggagactggagtcgttttcttattttattaatccactaaaaaacagATTAGTATATTCCCCTCGAAAGAATTCTACGAAGGTGTCCTAGAAGACGGGGAGGGACTCAGCAAGAAACGTCCATGGCATTCCTACAGCTGTTTCGGCC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|67021259|gb|CO450008.1|CO450008
EST:     CCGGTTTTCCAGTGCAAATGCTTAAAATCCAATATCGCATGCATCCAGAG                         ATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGG
genomic: CCGGTTTTCCAGTGCAAATGCTTAAAATCCAATATCGCATGCATCCAGAGgtgaatcttt ... tcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGG




















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 195

ATATGGAACGAGCTTGTTCAAAAGATTTCAAGCTGCCGGTTTTCCAGTGCAAATGCTTAAAATCCAATATCGCATGCATCCAGAGgtgaatcttt ... tcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 165

ATATGGAACGAGCTTGTTCAAAAGATTTCAAGCTGCCGGTTTTCCAGTGCAAATGCTTAAAATCCAATATCGCATGCATCCAGAGgtgaatcttt ... tcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 107

ATATGGAACGAGCTTGTTCAAAAGATTTCAAGCTGCCGGTTTTCCAGTGCAAATGCTTAAAATCCAATATCGCATGCATCCAGAGgtgaatcttt ... tcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 100

ATATGGAACGAGCTTGTTCAAAAGATTTCAAGCTGCCGGTTTTCCAGTGCAAATGCTTAAAATCCAATATCGCATGCATCCAGAGgtgaatcttt ... tcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atctgaagctggaaactggaatgcttggtgtaattcattatccattcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC
                                        tccattcct  CT-rich tract
                              taattcattat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgaatcttttatcctcagcatatctgaagctggaaactggaatgcttggtgtaattcattatccattcctatgcagATTAGTACATTCCCTTCAAAAGAATTTTATGAAGGGGTACTACAAGATGGGGAGGGCCTGAGCAGGAAACGTCCATGGCATTCCTACAGCTGCTTCGGAC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGCT