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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtaactctatctatggtcgcattttttcatcttgatcagagaatgttacgaccttcttttttattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA

Basic information

species Zea mays
transcript GRMZM2G096389_T02
intron # 16
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211368014|gb|FL470445.1|FL470445
EST:     CCCAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGTAGCTAGAGGGATT
genomic: CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
EST: gi|211368015|gb|FL470446.1|FL470446
EST:     CCAATATAAAAAGATTATCT-GGAGGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
genomic: ACAATATAAAAAGATTATCTGGGA-GGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
EST: gi|211023732|gb|FL449221.1|FL449221
EST:     CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
genomic: CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
EST: gi|76018788|gb|DT945958.1|DT945958
EST:     CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
genomic: CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
EST: gi|78120367|gb|DV538751.1|DV538751
EST:     CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
genomic: CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
EST: gi|60396686|gb|DN229553.1|DN229553
EST:     CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
genomic: CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
EST: gi|211122767|gb|FL444236.1|FL444236
EST:     CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAG                         GAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT
genomic: CACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 261

GTTGATGGGTACCTTCACAATGAGGAAGAAACACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 182

GTTGATGGGTACCTTCACAATGAGGAAGAAACACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 199

GTTGATGGGTACCTTCACAATGAGGAAGAAACACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 159

GTTGATGGGTACCTTCACAATGAGGAAGAAACACAATATAAAAAGATTATCTGGGAGGAAATGAACAAAGAGTACCTTGAGgtaagtaact ... tattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tcgcattttttcatcttgatcagagaatgttacgaccttcttttttattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA
             tcttgat  putative branch site (score: 159)
                                   ccttcttttttatttt  putative PPT
                                     ttcttttttattttac  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtaactctatctatggtcgcattttttcatcttgatcagagaatgttacgaccttcttttttattttacagGAACAAGCAGCCAAGGAAGCTTTGGCAGCTGAATTGGCAGCTAGAGGGATTGATCCAGAAGCGGGAAAGAAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAAGAA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGAAA