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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...agaatggtggtattcattgctagttacgtatattgtaactaaactattcttttctttgctaatctatgtgtatgttaaatttattgaatttctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG

Basic information

species Zea mays
transcript AC189879.3_FGT003
intron # 15
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: AC189879.3_FGT003 (Zea mays), 3'ss of exon 15
lower sequence: LOC_Os06g39640.1 (Oryza sativa), 3'ss of exon 16
---------------------------------------------------------------------------------------------------agaatggtggtattcattgctagttacgtatattgtaactaaactattcttttctttgctaatctatgtgtatgttaaatttattga----atttctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG
                                                                                                    |||   | || ||  ||| | | ||  |  | | | |  ||  |  | || |        || | | | | || |||   ||| |       |||||||| ||||| ||||| ||||||||||| ||||| ||||||||||| || ||||| ||||| ||||| |||
gtatgctactgcaaatactgtttataaactaacgtttgtagagtacagtagttagatatttgtggaatcaatttaatcctatgtatcttcaacaagtactgaacc-tagtgttt-ttgttcgctatttgaagtatga--aagttgctgttataggcttctatttgtatatctgctaatagtatcggtactgcttctcttctagGGATGGTGTCAGTGAAGGCCAATTCTACCAAGTTCTCCTCTATGAGTTGGATGCTATCCGCAAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|91877156|gb|EB407113.1|EB407113
EST:     TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAG                         GGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
genomic: TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
EST: gi|211241581|gb|FL211915.1|FL211915
EST:     TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAG                         GGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
genomic: TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
EST: gi|9566493|gb|BE476002.1|BE476002
EST:     TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAG                         GGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
genomic: TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
EST: gi|20301316|gb|BQ164259.1|BQ164259
EST:     TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAG                         GGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA
genomic: TCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 377

GGAGCTGTTAATATCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 94

GGAGCTGTTAATATCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 35

GGAGCTGTTAATATCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 67

GGAGCTGTTAATATCCTTCAGGAAGGCCACTGGGCAGAAGCCATTGAGAATAATATTCTACAGgtatgtttgt ... tctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

attcttttctttgctaatctatgtgtatgttaaatttattgaatttctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG
           tgctaat  putative branch site (score: 67)
                                           tttctcttcc  putative PPT
                   tatgtgtatgttaaat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agaatggtggtattcattgctagttacgtatattgtaactaaactattcttttctttgctaatctatgtgtatgttaaatttattgaatttctcttccagGGACGGTGTTAGTGAAGGCCAGTTCTATCAAGTTCTCCTTTACGAGTTAGATGCCATCCGTAAG

- - tggtggt
- - - - - - - - -tgctagt
- - - - - - - - - - - - - - - - - - aactaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gtgtatg