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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgcaacgttttcacttcataataatactgctggctacagatttttacatcttctcttgaacagttgttttgcattcttccatgcacattatcattttcccctgtaaacgctcttgtatgaaaaactacagtaaatgtgtagtgtaccatatttttgggttttgcgtgtgcgctaacctgggttttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG

Basic information

species Zea mays
transcript GRMZM2G143725_T02
intron # 12
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G143725_T02 (Zea mays), 3'ss of exon 12
lower sequence: LOC_Os04g04020.1 (Oryza sativa), 3'ss of exon 12
gtgcaacgttttcacttcataataatactgctggctacagatttttacatcttctcttgaacagttgttttgcattcttccatgcacattatcattttcccctgtaaacgctcttgtatgaaaaactacagtaaatgtgtagtgtacca---tatttttgggttttgcgtgtgcgctaacctgggttttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG
                                                                                   | || | | || |||   | |||||     ||| ||  |        || ||||||     | |||   || ||||| |||   |   |  |||||| |||| | | |||||| ||| ||||||||| ||||| || ||||  || ||||||||||||||||||||| |   |||  |||||| |||||||||||| |||||||| | ||  |
----------------------------------------------------------------------------------aggac-tgaacactttgttccgtaaa-----ttgaatctag-----ttgttaatgtgggtaattccaaactagttttgagtt--acaactatgctaac-tgggatatcttgcagGTTCAATTGAGAGAATGTGATAACGAGTACTCCAGAAATTTCATGAAAATACTAGGGACCCTAAGGGAGAGGGATCCTTCTTACCACCAGCTCTGCCGCG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60394551|gb|DN227421.1|DN227421
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|211056537|gb|FL371329.1|FL371329
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|83278191|gb|DV942199.1|DV942199
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|71445514|gb|DR826564.1|DR826564
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|211478592|gb|FL369719.1|FL369719
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|5915463|gb|AW053104.1|AW053104
EST:     ACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: ACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|211424168|gb|FL418879.1|FL418879
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|211169647|gb|FL398212.1|FL398212
EST:     GAACAACATTCTTGGAGTGAGCTTGGCAAACTTNCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: GAACAACATTCTTGGAGTGAGCTTGGCAAACTT-CCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
EST: gi|211504702|gb|FL413778.1|FL413778
EST:     TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAG                         ATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA
genomic: TGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 74

TGGCTTCACTTTCTTAGTGTGGTTAGGAAGGATGCTCCCACCTGAACTTGTGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 47

TGGCTTCACTTTCTTAGTGTGGTTAGGAAGGATGCTCCCACCTGAACTTGTGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 38

TGGCTTCACTTTCTTAGTGTGGTTAGGAAGGATGCTCCCACCTGAACTTGTGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 54

TGGCTTCACTTTCTTAGTGTGGTTAGGAAGGATGCTCCCACCTGAACTTGTGAACAACATTCTTGGAGTGAGCTTGGCAAACTTCCCTGACCTATCTAAGgtgcaacgtt ... tttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtagtgtaccatatttttgggttttgcgtgtgcgctaacctgggttttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG
                                cgctaac  putative branch site (score: 54)
                                            ttttgtt  putative PPT
          atattttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgcaacgttttcacttcataataatactgctggctacagatttttacatcttctcttgaacagttgttttgcattcttccatgcacattatcattttcccctgtaaacgctcttgtatgaaaaactacagtaaatgtgtagtgtaccatatttttgggttttgcgtgtgcgctaacctgggttttgttgcagATTCTATTGAGAGAGTGTGACAATGAGTTATCAAGAAATTTCATGAAAATACTAAGATACCTGCGGGAGAAGGATCCTTCTTATCACCAGCTATCCCTTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGAA