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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttaagctttttatttccttatggtcaggtaccctttatgtactgccgtgagatttaggccttaaaacttgctacatgtaaccattccctgttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA

Basic information

species Zea mays
transcript GRMZM2G052926_T02
intron # 12
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G052926_T02 (Zea mays), 3'ss of exon 12
lower sequence: LOC_Os05g36120.1 (Oryza sativa), 3'ss of exon 13
ttaagctttttatttccttatggtcaggtacccttta-tgtactgccgtgagatttaggccttaaaacttgctacat--gtaaccattccctgttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA
  |||| |  |||  |    |  | |||||  |     | |  |||     | |   ||||||||| ||||  |  |  ||    |   | | | |  | ||||||||  |||| |||||||||||||||||||| ||  | ||||| |||||||| |||||||| |||||||| |||||||||||||| || |||||||| |
--aagcctgatatgccaactta-taaggtattcaaagcttttgtgcaagatgttactggccttaaaccttggcatcttagttcttaactcatctgccttgcagAAAATAAATGAAGATAAGCTTTTCAATCTGTTCTCTCTATATGGGAACATAGTCCGAATCAAAATACTGCGCAACAAACCAGATCACGCCCTTGTTGAAA

upper sequence: GRMZM2G052926_T02 (Zea mays), 3'ss of exon 12
lower sequence: Vv18s0072g00310.t01 (Vitis vinifera), 3'ss of exon 12
------------------------------ttaagctttttatttccttatggtcaggtaccct---ttatgtactgccgtgagattta-----ggccttaaaacttgctacatgtaaccattccctgt-tctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA
                              ||   ||| || ||    | |  | |  |  |||   |||| |  ||   | | ||||      |||  |   ||    ||  | |    | |   | | ||  | |||| ||| ||||| |||||||||||||| ||||| ||| | ||||||||||| ||  |||| ||| | || || || || |||||||||||||| ||  | |
gtatgacttctctactcacccttcttcttcttcttcttcttcttcttctttatttatttttcctattttatcttttgtattaaaattttctgttggctcttctaccacttatgtttgggaactttttctgtcatttcagAGAATAGATGAAGATAAGCTTTTCAACCTGTTCTCCATCTATGGAAACATTGTCAGAATTAAGCTGCTCCGCAATAAGCCAGATCATGCACTAGTCCAAA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|31361372|gb|CD445729.1|CD445729
EST:     CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGAT                         AAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
genomic: CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
EST: gi|67014377|gb|CO443126.1|CO443126
EST:     CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGAT                         AAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
genomic: CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
EST: gi|60342999|gb|DN209972.1|DN209972
EST:     CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGAT                         AAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
genomic: CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
EST: gi|61118024|gb|DN558985.1|DN558985
EST:     CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGAT                         AAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
genomic: CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
EST: gi|61118023|gb|DN558984.1|DN558984
EST:     CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGAT                         AAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA
genomic: CTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 158

GGTTGCAATGATTGCGGCTGCATTTGGTGGAACTTTACCTCATGGAGTGACTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 53

GGTTGCAATGATTGCGGCTGCATTTGGTGGAACTTTACCTCATGGAGTGACTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 45

GGTTGCAATGATTGCGGCTGCATTTGGTGGAACTTTACCTCATGGAGTGACTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA


Block sizes: 46 (upstream exon), 48 (downstream exon)
Score: 45

GGTTGCAATGATTGCGGCTGCATTTGGTGGAACTTTACCTCATGGAGTGACTGGTACCAATGAACGGTGCACACTCATCGTCAGTAATCTGAATACTGATgtaagtcttg ... ttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cgtgagatttaggccttaaaacttgctacatgtaaccattccctgttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA
                             atgtaac  putative branch site (score: 45)
                                   ccattccctgttct  CT-rich tract
               ttaaaactt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttaagctttttatttccttatggtcaggtaccctttatgtactgccgtgagatttaggccttaaaacttgctacatgtaaccattccctgttctgtacagAAAATTGATGAGGATAAGCTTTTCAATCTGTTTTCCTTGTATGGAAACATAGTACGAATCAAGATACTGCGTAACAAACCAGATCATGCACTTGTTGAGA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGATCA
- - - - - - - - - - - - - - - - - - - -tactgcc
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - ctacatg